Genetic identification of tocopherol mutations in sunflower

Demurin, Ya.N.; Efimenko, S.G.; Peretyagina, T.M.

doi:10.2298/hel0440113d

Cited by 10 publications

(9 citation statements)

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“…These results were confirmed in the analysis of F 2 seeds, which also showed high gamma‐tocopherol contents of 960 ± 25 g kg −1 (T2100 × LG‐17), 970 ± 34 g kg −1 (T2100 × IAST‐1), and 980 ± 16 g kg −1 (T2100 × IAST‐540). Demurin et al (2004) evaluated the F 1 from a cross between the line VK 175, a line with tph 2 tph 2 genotype derived from LG‐17, and T2100, concluding that T2100 was allelic to tph 2. The results of the present research suggested that tph 2 alleles are present in the four lines.…”

Section: Resultsmentioning

confidence: 99%

“…Other genes controlling tocopherol biosynthesis have been mapped in sunflower. The Tph1 gene determining increased beta‐tocopherol content in sunflower lines LG‐15 and T589 (Demurin et al, 2004) was mapped to LG 1 of the sunflower genetic map (Vera‐Ruiz et al, 2006). The results of the present research demonstrated that both the Tph1 and the Tph2 genes will segregate independently, since they are located in different linkage groups, and the identification of molecular markers linked to them provides an efficient system to select the tph1tph1tph2tph2 genotype.…”

Section: Resultsmentioning

confidence: 99%

“…Also through the evaluation of the natural variability existing in germplasm collections, Velasco et al (2004a) developed the line T589, with a beta‐tocopherol content above 300 g kg −1 total tocopherols, and the line T2100, with a gamma‐tocopherol content above 850 g kg −1 Velasco and Fernández‐Martínez (2003) reported the presence of recessive alleles at a single locus underlying each of the modified tocopherol profiles, i.e., the increased beta‐tocopherol concentration in seeds of T589 and the high gamma‐tocopherol content in seeds of T2100. Comparative genetic studies concluded that tph1 alleles were present in both LG‐15 and T589 lines (Demurin et al, 2004; Vera‐Ruiz et al, 2005), and tph2 alleles were present in both LG‐17 and T2100 lines (Demurin et al, 2004).…”

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Genetic and Molecular Analysis of High Gamma‐Tocopherol Content in Sunflower

et al. 2006

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Sunflower (Helianthus annuus L.) seeds contain alpha-tocopherol as the major tocopherol derivative, which accounts for more than 900 g kg 21 total tocopherols. However, four sources of high gammatocopherol content (.850 g kg 21 ) have been developed. First studies on the lines LG-17 and T2100 concluded that the trait in both lines was determined by recessive alleles at the Tph2 locus. The objectives of the present research were (i) to conduct an allelic study on the other two lines, IAST-1 and IAST-540, (ii) to identify markers linked to the

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Genetic and Molecular Analysis of High Gamma‐Tocopherol Content in Sunflower

et al. 2006

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“…The genetic identification by the allelic test showed the new medium β-tocopherol mutation to be allelic to tph1 and the new high γ-tocopherol mutation to be allelic to tph2 (Demurin et al, 2004;Vera-Ruiz et al, 2005). The δ-tocopherol content in the phenotype of a recombinant double mutation achieved in segregants up to 68% (Velasco et al, 2004).…”

Section: Introductionmentioning

confidence: 98%

Expressivity of tocopherol mutations in sunflower

Demurin

Efimenko

Peretyagina

2006

Helia

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Influences of genetic background, ontogenesis and environment on tocopherol mutations in sunflower were estimated in this study. The content of α/β-tocopherols varied from 40/60 to 60/40% in seeds of different inbred lines containing the tph1 gene. The lines containing the tph2 gene ranged widely in α/γ-tocopherols, from 0/100 to 80/20%. A double mutation showed variability in different inbred lines in α/β/γ/δ-tocopherol contents from maximum expressivity of 0/0/60/40 to minimum 40/25/25/10%, due to incomplete expressivity of tph2. Seed maturation from 10 to 38 DAF influenced tocopherol composition in both normal and mutant genotypes by increasing the α-tocopherol content. The content varied from 81 to 97% in a normal genotype, from 33 to 50% in tph1 mutation and from 0 to 6% in tph2 mutation. Tocopherol mutations were shown to express their phenotype in different parts of a plant. All roots, hypocotyls, leaves, pollen and callus from the seeds, hypocotyls and leaves had normal, tph1, tph2 and double mutation tocopherol profiles depending on the genotype. The only exception was the absence of tph1 expressivity in the green tissue of the leaves. The experiment with day/night temperatures varying during seed development from 20/18 to 30/26°C showed an increased α-tocopherol content from 39 to 48% in tph1 mutation. Both a normal genotype (about 97% of α-tocopherol) and tph2 mutation (about 98% of γ-tocopherol) were constant in these two temperature regimes. Genetic background was the main factor that influenced the expressivity of tph2 in sunflower.

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“…Si l'on se réfère aux voies de synthèse, cette fonction concernerait probablement l'activité MPBQ méthyl transférase (figure 3). Le gène Tph2 contrôlerait le niveau d'α-et de γtocophérols, c'est-à-dire l'activité VTE3, tandis que Thp1 contrôlerait la méthylation du MBPQ, donc la balance entre les deux branches de la synthèse (VTE4) (Demurin et al, 2004). Ces deux locus seraient indépendants (Vera- Ruiz et al, 2006), car ils sont localisés dans des groupes de liaison différents.…”

Section: Variation De Profilunclassified

Que sait-on du déterminisme de la qualité des huiles du tournesol face aux nouvelles attentes ?

Gotor

Sarrafi

Maury

et al. 2010

OCL

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Sunflower oil is widely consumed in France and Spain. This oil has a good reputation among consumers, due to its high polyunsaturated fatty acid content. Thirty years after its discovery, the oleic mutation (high C18:1 contents) has now reached agricultural production, with fast increasing surfaces for the last three years. The challenge is now the production of very high oleic (> 90%) sunflowers and new fatty acid profiles, such as high linoleic, palmitic, stearic or stearic + oleic hybrids, which are of high interest for chemistry or food industry. The problem is now the stability of the expression in varying genetic backgrounds, and the understanding of environmental effects. Minor components are also a main strength of sunflower oil: its high tocopherols (Vitamin E) content (0,5 to 1%) is all the more interesting for nutritionists that they be almost exclusively α-tocopherol (> 95%), which is the most vitaminic active component. Nowadays, the unraveling of tocopherols biosynthesis and its key points of regulation is in progress. New tocopherol profiles have been recently obtained. Higher protective effects are attributed to γor δ-tocopherols. They could increase the stability of oils or margarines. Genetic progress for total content is possible, as in sunflower like in other oilseed crops, heritability is rather high (> 0.7). Sunflower oil also contains twice as much phystosterols as olive oil (3 vs 1,5%), and mainly β-sitosterol. Major mutations of this pathway can induce penalizing phenotypic modifications: 1) campesterol is the precursor of plant hormones brassinosteroids, and 2) stigmasterol/campesterol ratio is also implied in plant growth regulation. However, hybrids can significantly differ in their total content. Either for fatty acids or minor components, breeding programs for new profiles, or higher contents, need high throughout oil extraction and analysis. Chemometric analysis offer interesting solutions despite their need of large sets of reference analysis.

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Genetic identification of tocopherol mutations in sunflower

Cited by 10 publications

References 1 publication

Genetic and Molecular Analysis of High Gamma‐Tocopherol Content in Sunflower

Genetic and Molecular Analysis of High Gamma‐Tocopherol Content in Sunflower

Expressivity of tocopherol mutations in sunflower

Que sait-on du déterminisme de la qualité des huiles du tournesol face aux nouvelles attentes ?

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