Genetic immunization is a simple method for eliciting an immune response

Tang, Dale D.; DeVit, Michael J.; Johnston, Stephen Albert

doi:10.1038/356152a0

Cited by 1,359 publications

(604 citation statements)

References 6 publications

Supporting

Mentioning

580

Contrasting

Unclassified

Order By: Relevance

“…10 Soon, however, it turned out that other organs showed a similar ability for expression of a reporter gene. 11 The skin as a possible target tissue for genetic immunization was successfully tested by Tang et al, 12 by bombardment with gold microprojectiles coated with plasmid DNA. But gene expression in the skin was also observed when using needle injection of plasmid DNA, 13,14 especially when applying high-frequency puncturing of the skin.…”

Section: Introductionmentioning

confidence: 99%

Superiority of the ear pinna over muscle tissue as site for DNA vaccination

Förg

Hoegen

Dalemans³

et al. 1998

Gene Ther

View full text Add to dashboard Cite

Three different vaccination sites were compared for expression vector used. The cytomegalus virus promoter efficiency of immunization with naked DNA. Using the bacdriven pCMV␤ vector was superior to the Moloney murine terial lacZ gene as a model, all three sites of the mouse leukemia virus LTR driven BAG vector. LacZ DNA immuni-(skeletal muscle, dermis of abdominal skin or of the ear zation was also compared with cell-based vaccination with pinna) could express the gene product ␤-gal but varied in lacZ-transfected tumor cells, in which case again the pinna expression time with muscle tissue showing the longest was the best site for inducing strong immune responses. expression. Expression time, however, did not correlateTumor-specific T cell responses could also be well induced with immune response intensity. The ear pinna was by far in the pinna, leading to cytotoxic T lymphocyte induction the most effective and muscle the least effective priming and protective antitumor immunity. Thus, the pinna was site for specific humoral and cytotoxic T cell-mediated found to be a privileged site for induction of antitumor immune responses. Following intra-pinna DNA inoculation, responses and for genetic immunization, an important find-␤-gal expressing cells were detectable around the injection ing of immediate practical and potential future clinical site and in the major draining lymph node. Efficiency of implications. immunization was also dependent on the promoter and

show abstract

Section: Introductionmentioning

confidence: 99%

Superiority of the ear pinna over muscle tissue as site for DNA vaccination

Förg

Hoegen

Dalemans³

et al. 1998

Gene Ther

View full text Add to dashboard Cite

show abstract

“…We and others have developed a new strategy for the production of immunotherapy against infectious diseases as well as the treatment of speci®c types of cancers Davis et al, 1993;Kim et al, 1997b;Tang et al, 1992;Ulmer et al, 1993;Wang et al, 1993). This strategy is fundamentally di erent from previous vaccine approaches.…”

Section: Discussionmentioning

confidence: 99%

“…Nucleic acid immunization induces antigen-speci®c immune responses following injection of non-replicating plasmids directly into a host target tissue (Boyer et al, 1997;Davis et al, 1993;Fynan et al, 1993;Kim et al, 1997a,b;Kim and Weiner, 1998;Lu et al, 1995;Tang et al, 1992;Ulmer et al, 1993;Wang et al, 1993). Once injected, these non-replicating transcription units drive the synthesis of speci®c foreign proteins within the inoculated host.…”

Section: Introductionmentioning

confidence: 99%

Molecular and immunological analysis of genetic prostate specific antigen (PSA) vaccine

et al. 1998

View full text Add to dashboard Cite

Nucleic acid immunization has been investigated as immunotherapy for infectious diseases as well as for treating speci®c types of cancers. In this approach, nucleic acid expression cassettes are directly inoculated into the host, whose transfected cells become the production source of novel and possibly immunologically foreign protein. We have developed a DNA vaccine construct which encodes for PSA by cloning a cDNA for PSA into a mammalian expression vector under control of a CMV promoter. We investigated and characterized the immunogenicity of PSA DNA expression cassettes in mice. PSA-speci®c immune responses induced in vivo by immunization were characterized by enzyme-linked immunosorbent assay (ELISA), T helper proliferation cytotoxic T lymphocyte (CTL), and¯ow cytometry assays. We observed a strong and persistent antibody response against PSA for at least 180 days following immunization. In addition, a signi®cant T helper cell proliferation was observed against PSA protein. Using synthetic peptides spanning the PSA open frame, we identi®ed four dominant T helper epitopes of PSA. Furthermore, immunization with PSA plasmid induced MHC Class I CD8+ T cell-restricted cytotoxic T lymphocyte response against tumor cell targets expressing PSA. The prostate represents a very speci®c functional organ critical for reproduction but not for the health and survival of the individual. Understanding the immunogenicity of PSA DNA immunization cassettes o ers insight into the possible use of this tumorassociated antigen as a target for immunotherapy. These results demonstrate the ability of the genetic PSA to serve as a speci®c immune target capable of generating both humoral and cellular immune responses in vivo.

show abstract

“…It was started from studies of the early 90s, 44,45 and after a short time became a quickly developing field of gene transfer-mediated immunostimulation with special emphasis on infections and malignances. The main advantages of DNA vaccination are relative safety (absence of attenuated or killed pathogens), low cost (missing step of protein preparation), and long storage without loss of activity.…”

Section: Vaccination With Dna Encoding Cancer Antigensmentioning

confidence: 99%