Genetic Improvement of Rice for Bacterial Blight Resistance: Present Status and Future Prospects

Fiyaz, R. Abdul; Shivani, D.; Chaithanya, K.; Mounika, K.; Chiranjeevi, M.R.; Laha, G. S.; Viraktamath, B. C.; Rao, L. V. Subba; Sundaram, R. M.

doi:10.1016/j.rsci.2021.08.002

Cited by 47 publications

(38 citation statements)

References 115 publications

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“…The current study tested five sets of molecular markers that had previously shown functional polymorphisms for each of the target traits, including Wx -SSR, fgr -SNP, pTA248, RM8225, and RM202 [ 19 , 20 , 21 , 22 , 23 , 24 ]. All of the selected markers showed functional polymorphisms with expected sizes of amplified products ( Table 5 ), indicating that they can be employed for multiplexing.…”

Section: Resultsmentioning

confidence: 99%

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Hanafiah

Cheng

Lim

et al. 2022

Life

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While previous research has demonstrated that multiplex polymerase chain reaction (PCR) can be a cost-effective approach to detect various genes in crops, the availability of multiplex assays to simultaneously screen both grain quality and biotic stress resistance traits in rice (Oryza sativa) is limited. In this work, we report six novel multiplex assays that use a universal protocol to detect major rice grain quality (amylose content and fragrance) and biotic stress (blast, sheath blight, and bacterial leaf blight) traits with amplified products consisting of up to four primer pairs that can be analyzed using a standard agarose-based gel electrophoresis system. Recent studies have suggested that weedy rice has novel sources of disease resistance. However, an intensive screening of weedy biotypes has not been reported in Malaysia. Accordingly, we employed one of the developed multiplex assays to screen reported genes or quantitative trait loci (QTLs) associated with blast, sheath blight, and bacterial leaf blight diseases in 100 weedy rice biotypes collected from five local fields, with phenotyping performed to validate the genotyping results. In conclusion, our universal multiplex protocol is effective for the large-scale genotyping of rice genetic resources, and it can be employed in routine molecular laboratories with limited resources.

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Section: Resultsmentioning

confidence: 99%

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Hanafiah

Cheng

Lim

et al. 2022

Life

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“…qBBR11, which was determined to be a major resistance gene, has an LOD score of 5.47, and 24.5% of the phenotypic variation can be explained. BB disease resistance genes have been reported in various populations of rice cultivars through previous studies [ 58 ], and 26 dominant genes and 14 recessive genes were studied [ 7 ]. Among the 12 rice chromosomes, the genes are distributed on 10 chromosomes except for chromosomes 9 and 10, and genes for BB resistance are distributed intensively on chromosomes 4 and 11 [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

“…oryzae ( Xoo ), is a major cause of severe losses in rice yields, mainly in Asia, and is among the most devastating diseases [ 6 ]. In particular, Asia is a region in which more than 90% of the world’s rice production is cultivated and exported, so the development of BB disease-resistant varieties and the discovery of resistance genes is an essential goal [ 7 , 8 ]. BB diseases interfere with the growth of rice through the proliferation of bacteria, which invade through the hands and wounds of individuals.…”

Section: Introductionmentioning

confidence: 99%

Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice

Park

Lee

et al. 2022

Plants

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Bacterial blight (BB) disease, caused by Xanthomonas oryzae pv. oryzae (Xoo), is among the major factors that can cause rice yields to decrease. To address BB disease, researchers have been looking for ways to change pesticides and cultivation methods, but developing resistant cultivars is the most effective method. However, the resistance and genetic factors of cultivars may be destroyed due to the emergence of new Xoo species caused by recent and rapid climate changes. Therefore, breeders need to identify resistance genes that can be sustained during unpredictable climate changes and utilized for breeding. Here, qBBR11, a quantitative trait locus (QTL) for resistance to BB disease, was detected in KJ (Korea Japonica varieties) 11_067 to KJ11_068 on chromosome 11 in a population derived by crossing JJ (Jeonju) 623 and HR(High resistant)27,195, which possess similar genetic backgrounds but different degrees of resistance to BB disease. qBBR11 was reduced from 18.49–18.69 Mbp of chromosome 11 to 200 kbp segment franked. In this region, 16 candidate genes were detected, and we identified 24 moderate-impact variations and four high-impact variations. In particular, high-impact variations were detected in Os11g0517800 which encode the domain region of GCN2 which is the eIF-2-alpha kinase associated with the resistance of abiotic/biotic stress in rice. In JJ623, which is moderately resistant to BB disease, a stop codon was created due to single nucleotide polymorphism (SNP). Therefore, compared with HR27195, JJ623 has weaker resistance to BB disease, though the two have similar genetic backgrounds. The results suggest that variation in the qBBR11 region regulates an important role in improving resistance to BB diseases, and qBBR11 is useful in providing an important resource for marker-assisted selection to improve mechanisms of resistance to BB disease.

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“…Rice is an important food crop and is the staple food of more than 50% of the world's population. [1][2][3] However, RFS, one of the main diseases of rice, can cause a serious decline in the quality and yield of rice. 4,5 In addition, in the early stage of infection, a large number of secondary conidia and hyphae are rapidly produced on the surface of rice spikelets after the conidia of Ustilaginoidea virens germinate.…”

Section: Introductionmentioning

confidence: 99%

Collection, nucleic acid release, amplification, and visualization platform for rapid field detection of rice false smut

Sun

Shen

et al. 2023

Lab Chip

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Genetic Improvement of Rice for Bacterial Blight Resistance: Present Status and Future Prospects

Cited by 47 publications

References 115 publications

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice

Collection, nucleic acid release, amplification, and visualization platform for rapid field detection of rice false smut

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