2014
DOI: 10.1039/c3sc51617b
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Genetic incorporation of unnatural amino acids biosynthesized from α-keto acids by an aminotransferase

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Cited by 29 publications
(22 citation statements)
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“…Further development of chemical and enzymatic routes for the synthesis of UAAs in an easy and cheap way will be a milestone in enzyme engineering. Alternatively, host cells can be engineered for the biosynthesis of UAA such as phenylalanine and pyrrolysine analogs and their subsequent incorporation into target proteins [89][90][91]. Ultimately, evolving host cells for the metabolic production of UAAs from cheap sources and concurrently incorporating them into target enzymes will lead to a boom in the industrial applications of engineered enzymes.…”
Section: Concluding Remarks and Future Perspectivesmentioning
confidence: 98%
“…Further development of chemical and enzymatic routes for the synthesis of UAAs in an easy and cheap way will be a milestone in enzyme engineering. Alternatively, host cells can be engineered for the biosynthesis of UAA such as phenylalanine and pyrrolysine analogs and their subsequent incorporation into target proteins [89][90][91]. Ultimately, evolving host cells for the metabolic production of UAAs from cheap sources and concurrently incorporating them into target enzymes will lead to a boom in the industrial applications of engineered enzymes.…”
Section: Concluding Remarks and Future Perspectivesmentioning
confidence: 98%
“…Another pioneering result in intracellular ncAA synthesis and simultaneous SCS was reported by Jung et al . The authors were able to biosynthesize the aromatic amino acids p ‐acetyl‐phenylalanine ( p ‐AcF), p ‐cyano‐phenylalanine ( p ‐CNF), p ‐phenyl‐phenylalanine ( p ‐PhF), and p ‐azido‐phenylalanine ( p ‐AzF) from easily accessible α‐keto acids and a biocatalyst (Table , entry 2).…”
Section: Pairing Intracellular Synthesis Of Ncaas Genetic‐code Expanmentioning
confidence: 99%
“…To ensure sufficient intracellular concentrations,t he ncAA analogues are supplied in excess in the culture and this may become expensive,inparticular for scale-up fermentations.However, this issue was recently addressed by engineering the expression host with metabolic pathways for the intracellular production of ncAAs. [44,75,88] Similarly, E. coli strains have been engineered for the SCS production of proteins containing Tyr, [177] Phe, [178] and Pyl [179] analogues.W ithout doubt, an expanded genetic code in combination with metabolic engineering would further diversify the chemical arsenal for the large-scale production of useful xenoproteins,w hich are today still the exclusive domain of proof-of-principle experiments.…”
Section: Angewandte Chemiementioning
confidence: 99%