Genetic Individualization of Domestic Cats Using Feline STR Loci for Forensic Applications

Menotti‐Raymond, Marilyn; Va, David; Jc, Stephens; La, Lyons; O’Brien, SJ

doi:10.1520/jfs14258j

Cited by 67 publications

(44 citation statements)

References 41 publications

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“…Of the 17 loci, 14 were unlinked on a genetic recombination map (Menotti- Raymond et al 1999), one was not mapped (FCA166), and one pair was less than 20 cM apart (FCA43 and FCA117). My PCR amplifications followed conditions described by Menotti-Raymond et al (1997).…”

Section: Microsatellitesmentioning

confidence: 99%

Fine scale population genetic structure of pumas in the Intermountain West

Loxterman

2011

Conserv Genet

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In this study, I examined the population genetic structure of subpopulations of pumas (Puma concolor) in Idaho and surrounding states. Patterns of genetic diversity, population structure, levels of inbreeding, and the relationship between genetic differentiation and dispersal distance within and between 15 subpopulations of pumas were compared. Spatial analyses revealed that the Snake River plain was an important barrier to movement between northern and southern regions of Idaho. In addition, subpopulations south of the Snake River plain exhibited lower levels of genetic diversity, higher levels of inbreeding, and a stronger pattern of isolation by distance relative to subpopulations north of the Snake River plain. Lower levels of diversity and restricted gene flow are likely the result of historically lower population sizes in conjunction with more recent changes in habitat use and available dispersal corridors for movement. The subdivision of puma populations north and south of the Snake River plain, along with the patterns of genetic diversity within regions, indicate that landscape features are affecting the population genetic structure of pumas in Idaho. These results indicate that information about the effects of landscape features on the distribution of genetic diversity should be considered when designing plans for the management and conservation of pumas.

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Section: Microsatellitesmentioning

confidence: 99%

Fine scale population genetic structure of pumas in the Intermountain West

Loxterman

2011

Conserv Genet

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“…The majority of transferred cat hairs have been naturally shed, and lack the large root bulb required for reliable high-discrimination STR profiling [3]. However, even hair shafts typically contain enough mtDNA for sequencing, which is still capable of excluding many potential sources despite lower discriminating power than nuclear DNA [4].…”

Section: Introductionmentioning

confidence: 99%

Application of a mitochondrial DNA control region frequency database for UK domestic cats

Ottolini

Lall

Sacchini³

et al. 2017

Forensic Science International: Genetics

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DNA variation in 402 bp of the mitochondrial control region flanked by repeat sequences RS2 and RS3 was evaluated by Sanger sequencing in 152 English domestic cats, in order to determine the significance of matching DNA sequences between hairs found with a victim's body and the suspect's pet cat. Whilst 95% of English cats possessed one of the twelve globally widespread mitotypes, four new variants were observed, the most common of which (2% frequency) was shared with the evidential samples. No significant difference in mitotype frequency was seen between 32 individuals from the locality of the crime and 120 additional cats from the rest of England, suggesting a lack of local population structure. However, significant differences were observed in comparison with frequencies in other countries, including the closely neighbouring Netherlands, highlighting the importance of appropriate genetic databases when determining the evidential significance of mitochondrial DNA evidence.

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“…Their main advantages are that they can be easily assayed by PCR, they are expressed co-dominantly in phenotype, and they are highly polymorphic in most organisms. Recently, there has been an increase in the forensic application of STRs to cases involving non-human DNA, for example, wild animals hunted down by poachers, domestic canines, felines, and even plants [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%