Genetic information of three pure lines of Porphyra yezoensis (Bangiales, Rhodophyta) obtained by AFLP analysis

Iitsuka, Osamu; Nakamura, Kayo; Okamoto, Nobuaki; Saga, Naotsune

doi:10.1046/j.1444-2906.2002.00540.x

Cited by 26 publications

(16 citation statements)

References 16 publications

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“…AFLP has already been employed for marine and freshwater macroalgae (e.g. Donaldson et al, 1998Donaldson et al, , 2000Kusumo & Druehl, 2000;Iitsuka et al, 2002;Mannschreck et al, 2002;Schaeffer et al, 2002;Murphy & Schaffelke, 2003). Finally we used AFLP data to estimate nucleotide diversity (p) within and between species using two recently developed methods (Innan et al, 1999;Mougel et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Nucleotide diversity within and between four species ofLaminaria(Phaeophyceae) analysed using partial LSU and ITS rDNA sequences and AFLP

Erting¹,

Daugbjerg²,

Pedersen³

2004

European Journal of Phycology

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In order to estimate the nucleotide diversity of the four kelp species Laminaria digitata, L. hyperborea, L. saccharina and L. faeroensis, three approaches were used. First we sequenced 1320 base pairs (bp) of nuclear-encoded large subunit (LSU) rDNA, which included some of the most variable domains. These sequences discriminated between L. digitata, L. hyperborea and L. saccharina, but L. faeroensis was identical to L. saccharina. A phylogeny inferred based on 672 bp of the nuclearencoded non-coding internal transcribed spacer regions (ITS1 and ITS2) rDNA, which are more variable than LSU rDNA, likewise failed to resolve a monophyletic L. saccharina with respect to L. faeroensis. Thirdly, we tested the usefulness of the amplified fragment length polymorphism (AFLP) technique to provide a resolved phylogeny for 43 Laminaria specimens. Laminaria digitata and L. hyperborea were resolved as strongly supported monophyletic groups. A third robust clade consisted of L. saccharina plus L. faeroensis. Within this clade, L. faeroensis samples were grouped with bootstrap support of 80%, but the L. saccharina clade was less well supported. The AFLP data in combination with rDNA sequences indicated subspecies status for L. faeroensis. AFLP analyses of different morphotypes of L. digitata and L. saccharina from Danish coastal waters did not reveal any differences. However, L. digitata from the Faroe Islands was genetically distinct from other samples.

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Section: Introductionmentioning

confidence: 99%

Nucleotide diversity within and between four species ofLaminaria(Phaeophyceae) analysed using partial LSU and ITS rDNA sequences and AFLP

Erting¹,

Daugbjerg²,

Pedersen³

2004

European Journal of Phycology

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“…Molecular analysis has been used in the genetics and breeding of Porphyra since the end of the twentieth century (Jia et al 2000). Most previous reports were focused on the identification, protection, construction, and classification of different strains (Iitsuka et al 2002;Kunimoto et al 2003;Liu et al 2004;Sun et al 2005;Weng et al 2005;Qiao et al 2007). No molecular analysis focused on the reason why interspecific hybridization strains can bear offspring.…”

Section: Introductionmentioning

confidence: 97%

Analysis of hybridization strains of Porphyra based on rbcL gene sequences

Yang

Zhu

et al. 2010

J Appl Phycol

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Two strains, which are the free-living conchocelis of Porphyra yezoensis Ueda and Porphyra haitanensis T. J. Chang et B. F. Zheng, and four "interspecific hybridization" strains of these two species are investigated. The rbcL genes of 11 samples were amplified and sequenced, each of which were about 1,400 bp with a good specificity. The results of pair-wise distance matrix and multi-alignment showed that pair-wise distance were small while homologous index was large between strains Y-2066, Y-k2001, and Y-H001. These two indexes showed the same level as the above between strains H-2001, Y-hyC 1 , and Y-hyC 2 ; however, the distances were larger between three former strains and three latter strains. Cluster trees showed the same trend. Fertile strains appeared after 2 years of culture of unfertile interspecific hybridization conchocelis and were separated from that based on different colors. Our finding that these fertile strains can bear offspring is important for understanding the interspecies hybridization of Porphyra. Molecular analysis of the fertile strains based on rbcL gene showed maternal inheritance. We inferred that somatic recombination was one of the reasons making interspecific hybridization strains fertile.

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“…In this genus, cultivated P. yezoensis Ueda is one of the most economically important marine crops in East Asia (Niwa and Aruga 2003;Niwa et al 2008). Since the 1960s with the development of synthetic fiber nets, strain-preservation and artificial seeding in Porphyra mariculture, nearly 60 strains of P. yezoensis have been isolated and used for cultivation in Japan, China and South Korea Iitsuka et al 2002;Hwang et al 2005;Weng et al 2005;Hu et al 2007;Niwa et al 2008Niwa et al , 2009. Although these cultivars have considerably contributed to increasing yields, some subsequent problems occurred in nori farms.…”

Section: Introductionmentioning

confidence: 99%

NrDNA internal transcribed spacer revealed molecular diversity in strains of red seaweed Porphyra yezoensis and genetic insights for commercial breeding

Liu

Shao

et al. 2010

Genet Resour Crop Evol

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Unraveling the cryptic genetic diversity and selective breeding network in various Porphyra strains is of significance for conservation and utilization of economically important nori crops, for both current and future needs. Here, we used nuclear ribosomal spacer (ITS1) region to investigate the genetic variation and intra-specific relatedness of 59 Porphyra yezoensis Ueda specimens worldwide using phylogenetic and parsimony genealogical approaches. 23 nrDNA ITS1 genotypes were revealed and clustering analysis grouped them into two distinct clades. High genetic diversity was detected in wild P. yezoensis strains from Miyagi and Hokkaido Prefectures in Japan, while the cultivated strains from China and South Korea exhibited relatively higher genetic diversity likewise, which provided crucial genetic insights for future commercial breeding of P. yezoensis on a global scale. In addition, phylogenetic study has revealed the genetic relationship of strains with unknown parentage to those with known parentage, and also ITS1 sequence pattern could be correlated with the geographic origin of P. yezoensis specimens. All these pedigree information generated from this research can be used to select parents for inter-specific or intra-specific selective breeding and cross breeding to maximize the preservation of stock resource and sustainable development of nori industry.

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Genetic information of three pure lines of Porphyra yezoensis (Bangiales, Rhodophyta) obtained by AFLP analysis

Cited by 26 publications

References 16 publications

Nucleotide diversity within and between four species ofLaminaria(Phaeophyceae) analysed using partial LSU and ITS rDNA sequences and AFLP

Nucleotide diversity within and between four species ofLaminaria(Phaeophyceae) analysed using partial LSU and ITS rDNA sequences and AFLP

Analysis of hybridization strains of Porphyra based on rbcL gene sequences

NrDNA internal transcribed spacer revealed molecular diversity in strains of red seaweed Porphyra yezoensis and genetic insights for commercial breeding

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