Genetic interpretation of polytene chromosomes banding pattern

Жимулев, И. Ф.; Pokholkova, Galina V.; Bgatov, A. V.; Semeshin, V. F.; Umbetova, G. H.; Belyaeva, E. S.

doi:10.1007/bf00777469

Cited by 15 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in the hsp60 F409 flies, hsp60 G93 homozygous mutant animals die early in embryogenesis [15], embryos are amorphous and their nuclei are distributed randomly (data not shown). The hsp60 G93 sequence did not show any change in the ORF.…”

Section: Resultsmentioning

confidence: 72%

“…The wild‐type stock was OreR. The hsp60 F409 and hsp60 G93 alleles were maintained in the presence of the FM6 balancer [15,16]. Flies were reared on standard yeast dextrose medium at 25°C.…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, we do not find functional binding sites for heat shock factor, confirming our previous data [13] that neither the transcript nor the protein are heat inducible. We also present the molecular characterization of two ethyl methane sulfonate embryonic lethal alleles of the hsp60 gene that have been described previously [14,15]. The molecular analysis of the hsp60 F409 allele shows a C to T transition that produces an evolutionarily conserved serine change to leucine in amino acid 154.…”

Section: Introductionmentioning

confidence: 92%

See 2 more Smart Citations

Molecular characterization of the 5′ control region and of two lethal alleles affecting the hsp60 gene in Drosophila melanogaster

Perezgasga

Segovia²,

Zurita

1999

FEBS Letters

View full text Add to dashboard Cite

The chaperonins are evolutionarily conserved essential cellular proteins that help folding newly synthesized or translocated proteins, spending ATP. We present here the molecular analysis of the hsp60 gene promoter region and of two Drosophila hsp60 ethyl methane sulfonate embryonic lethal alleles that have an identical phenotype. No heat shock element sequences were found in the 5P P region, supporting previous data (Kozlova, T. et al., 1997) which suggests that mitochondrial Drosophila melanogaster HSP60.1 is not heat inducible. By sequencing the lethal allele's entire open reading frame (ORF), we found a C-T transition in the hsp60 F409 allele that produces a serine to leucine change, apparently distorting the protein equatorial domain structure. No changes were found in the hsp60 G93 ORF. However, an analysis of the heterogeneous nuclear RNA levels showed a reduction of the hsp60 transcript in hsp60 G93 flies as compared to the wild-type. These data suggest that although the defects in the hsp60 gene produced by these alleles are at different levels, both behave as null mutations.z 1999 Federation of European Biochemical Societies.

show abstract

Section: Resultsmentioning

confidence: 72%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Molecular characterization of the 5′ control region and of two lethal alleles affecting the hsp60 gene in Drosophila melanogaster

Perezgasga

Segovia²,

Zurita

1999

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…The chromosomes of multicellular animals have a regular and inheritable physical organization. This was first recognized in studies on the lampbrush chromosomes in amphibian oocytes and the polytene chromosomes of insects (Callan, 1963;Nora et al, 2020;Zhimulev and Belyaeva, 1991;Zhimulev et al, 1983). Subsequent research has shown that the architectural features inferred from analysis of lampbrush and polytene chromosomes are present in chromosomes throughout much of the animal kingdom.…”

Section: Introductionmentioning

confidence: 93%

Chromosome Structure I: Loop extrusion or boundary:boundary pairing?

Bing,

Ke,

Fujioka

et al. 2023

Preprint

View full text Add to dashboard Cite

Two different models have been proposed to explain how the endpoints of chromatin looped domains (“TADs”) in eukaryotic chromosomes are determined. In the first, a cohesin complex extrudes a loop until it encounters a boundary element roadblock, generating a stem-loop (and an unanchored loop). In this model, boundaries are functionally autonomous: they have an intrinsic ability to halt the movement of incoming cohesin complexes that is independent of the properties of neighboring boundaries. In the second, loops are generated by boundary:boundary pairing. In this model, boundaries are functionally non-autonomous, and their ability to form a loop depends upon how well they match with their neighbors. Moreover, unlike the loop-extrusion model, pairing interactions can generate both stem-loops and circle-loops. We have used a combination of MicroC to analyze how TADs are organized and experimental manipulations of theeven skippedTAD boundary,homie, to test the predictions of the “loop-extrusion” and the “boundary-pairing” models. Our findings are incompatible with the loop-extrusion model and instead suggest that endpoints of TADs in flies are determined by a mechanism in which boundary elements physically pair with their partners, either head-to-head, or head-to-tail, with varying degrees of specificity. How the partners find each other is not clear but is unlikely to require a loop extrusion mechanism.

show abstract

“…Results from in situ hybridization to polytene chromosomes of a clone spanning the breakpoint site suggested that the break lies more proximally, most probably in the very thin bands around 9E6, which is more in line with the original localization in 9 E 7.8 (ref. 38). This observation is further confirmed by the fact that the above clone was obtained from a chromosomal walk extending proximally from an entry point at 9E3.4.…”

mentioning

confidence: 93%

Molecular analysis of a candidate gene for the reproductive isolation between sibling species ofDrosophila

Hutter

Karch

1994

Experientia

View full text Add to dashboard Cite

The X-linked gene Hmr in Drosophila melanogaster, when mutated, rescues otherwise inviable interspecific hybrids from crosses between D. melanogaster and any of its three most closely related species D. simulans, D. mauritiana and D. sechellia. DNA from the site of a breakpoint at the putative locus of the gene has been cloned, and results of transcription and sequence analyses are presented. Three distinct mRNAs are transcribed from this locus, two of which are abundantly expressed throughout life. A third transcript, which is larger but rarer, appears to be disrupted by at least one of the two known mutations of Hmr. The gene encodes a mitochondrial ADP/ATP translocator protein, which plays an essential role in maintaining metabolic energy. Analysis of several cDNAs suggested that the rescue of hybrids may be dependent on mutations in the variable 3' end region of this gene, affecting the level and/or the stability of the largest messenger RNA.

show abstract

Genetic interpretation of polytene chromosomes banding pattern

Cited by 15 publications

References 11 publications

Molecular characterization of the 5′ control region and of two lethal alleles affecting the hsp60 gene in Drosophila melanogaster

Molecular characterization of the 5′ control region and of two lethal alleles affecting the hsp60 gene in Drosophila melanogaster

Chromosome Structure I: Loop extrusion or boundary:boundary pairing?

Molecular analysis of a candidate gene for the reproductive isolation between sibling species ofDrosophila

Contact Info

Product

Resources

About