Genetic linkage mapping for molecular dissection of chilling requirement and budbreak in apricot (<i>Prunus armeniaca</i>L.)

Olukolu, Bode A.; Trainin, Taly; Fan, Shenghua; Kole, Chittaranjan; Bielenberg, Douglas G.; Reighard, G.L.; Abbott, Albert G.; Holland, Doron

doi:10.1139/g09-050

Cited by 85 publications

(65 citation statements)

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“…Surprisingly, on the BC 2 , these QTLs were not detected in 2001, 2005 and 2006 but were detected with the highest effect in 2007 and 2008 and also with the multi-year analysis, suggesting a higher interaction with climatic conditions for this progeny. In the apricot 'Perfection' , two QTLs controlling chilling requirements and bud break (Olukolu et al, 2009) were also detected on LG6 (Supplementary Figure S1). These results suggest that some of the genes involved in the control of the flowering date may be the same in different species and that chilling requirements and flowering date may be determined by the same or tightly linked genes.…”

Section: Qtl Analysesmentioning

confidence: 99%

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Comparison of the genetic determinism of two key phenological traits, flowering and maturity dates, in three Prunus species: peach, apricot and sweet cherry

et al. 2012

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The present study investigates the genetic determinism of flowering and maturity dates, two traits highly affected by global climate change. Flowering and maturity dates were evaluated on five progenies from three Prunus species, peach, apricot and sweet cherry, during 3-8 years. Quantitative trait locus (QTL) detection was performed separately for each year and also by integrating data from all years together. High heritability estimates were obtained for flowering and maturity dates. Several QTLs for flowering and maturity dates were highly stable, detected each year of evaluation, suggesting that they were not affected by climatic variations. For flowering date, major QTLs were detected on linkage groups (LG) 4 for apricot and sweet cherry and on LG6 for peach. QTLs were identified on LG2, LG3, LG4 and LG7 for the three species. For maturity date, a major QTL was detected on LG4 in the three species. Using the peach genome sequence data, candidate genes underlying the major QTLs on LG4 and LG6 were investigated and key genes were identified. Our results provide a basis for the identification of genes involved in flowering and maturity dates that could be used to develop cultivar ideotypes adapted to future climatic conditions. Heredity (2012) 109, 280-292; doi:10.1038/hdy.2012.38; published online 25 July 2012Keywords: Prunus; phenology; flowering date; maturity date; QTL analyses; candidate gene INTRODUCTIONIn the context of global climate change, flowering phenology of deciduous tree species is crucial as it may affect their productivity. In fruit tree orchards, flowering phenology has an indirect influence on spring frost damage, pollination, dormancy and maturity. Even though in a warming scenario, the current risk of frost damage might remain a preoccupation for growers subsequently to advanced flowering time and more irregularities of temperature conditions. Moreover, new risks are emerging as disruptions in floral phenology synchronization, which may disturb pollination for varieties that necessitate cross pollination. In addition, marked changes in the order of flowering time within a varietal range or between adjacent cropping areas may modify the orders of fruit maturity time and consequently disturb commercial specificities.The Prunus genus, within the Rosaceae family, is characterized by species that produce drupes as fruit, and can be divided into three major subgenera: Amygdalus (peach (Prunus persica (L.) Batsch) and almond (Prunus dulcis Mill.)), Prunophora (apricot (Prunus armeniaca L.)), Cerasus (sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.)). All these species are grown in climates with well-differentiated seasons where they have adapted to survive to low winter temperatures and summer drought. In Prunus, as in most woody perennials, the physiology and biochemistry of the flowering

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Section: Qtl Analysesmentioning

confidence: 99%

“…These colocalizations suggested that a unique temperature sensor regulates both traits. In apricot, chilling requirements and bud break were analyzed in an F 1 population 'A.1740' Â 'Perfection' and QTLs were detected in all LGs except LG3 and LG4 (Olukolu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the genetic determinism of two key phenological traits, flowering and maturity dates, in three Prunus species: peach, apricot and sweet cherry

et al. 2012

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“…Bud outgrowth did not resume during the period of observation (Oct. to the following Aug.), and most QTL (LOD > 18) in G1 was also detected as a QTL for heat requirement and blooming date, suggesting that there may be one unified temperature sensing system in this region that regulates CR and leads to blooming. In apricot, the use of F 1 pseudo-testcross progenies identified QTLs associated with the CR of vegetative buds, in G1, G2, G3, G5, and G8 (Olukolu et al, 2009). In almond, a major QTL for CR of flower buds was located in G4, and minor QTLs were located in G1, G3, and G7 (Sánchez-Pérez et al, 2012).…”

Section: Seasonal Growth-dormancy Phase Transition Ofmentioning

confidence: 99%

Regulation of Bud Dormancy and Bud Break in Japanese Apricot (Prunus mume Siebold ^|^amp; Zucc.) and Peach [Prunus persica (L.) Batsch]: A Summary of Recent Studies

Yamane

2014

J. Japan. Soc. Hort. Sci.

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Bud dormancy allows most deciduous fruit tree species to avoid injury in unsuitable environments, synchronize their annual growth, and adapt to a temperate zone climate. Because bud dormancy affects next season's fruit production and vegetative growth, it is considered one of the most important physiological factors that control fruit production. Recent global climate changes require us to better understand the genetic factors regulating bud dormancy, especially those that induce dormancy release and subsequent bud break. In this review, environmental factors that affect the seasonal dormancy depth of Japanese apricot (P. mume Siebold & Zucc.) and peach [P. persica (L.) Batsch] are first outlined. Next, recent progress of genetic, biochemical, and molecular biological studies of Prunus dormancy regulation is described. Recent advances in functional genomics have promoted the discovery of gene function and gene networks associated with bud dormancy regulation. A group of candidate genes for bud dormancy regulation, the DORMANCY-ASSOCIATED MADS-box (DAM) genes in Prunus, are focused. Recently reported expressional analysis suggests a significant role for DAMs in dormancy release and bud break of Japanese apricot and peach vegetative buds. Transformation studies of PmDAM6 have demonstrated that it has an inhibitory effect on the apical growth of poplar (Populus spp.). As bud dormancy is a quantitative polygenic trait, not only DAMs, but also other genes and gene networks appear to regulate bud dormancy. Ongoing and future studies will undoubtedly facilitate the unveiling of the molecular aspects of bud dormancy regulation in temperate fruit tree species of Prunus.

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“…In apricot, QTLs analysis of a cross between "Perfection" (high chilling variety) and "A1740" (low chilling variety) showed the most significant QTLs on LG1, LG5 and LG7 (Olukolu et al, 2009);…”

Section: Genetic Studies and Mapping Of Dormancy Associated Locimentioning

confidence: 99%

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Leida¹

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En estos cuatros años en los que he estado trabajando, he conocido a mucha gente que me ha ayudado en distintas formas para la realización de esta tesis y por eso he pensado en agradecerlos.En primer lugar quería dar las gracias a mis directores por la dedicación que me han prestado. A Gerardo y Marisa por darme la oportunidad de trabajar en el IVIA y de desarrollar esta tesis. A Gabino por haber estado ahí en todo momento, para enseñar y contestar a mis preguntas, sin su apoyo no habría podido llegar hasta aquí. ABSTRACTDormancy is one of the most important adaptive mechanisms developed by perennial plants, in order to survive the low temperatures of autumn and winter in temperate climates. The study of the genes regulated during dormancy release is crucial to understand the process, with the final objective of the development of new varieties with a better adaptation to certain environments; and this is particularly important considering the increasing economical weight of fruit crops in low and medium chilling regions as the Mediterranean area. We focused on the molecular and physiological mechanisms underlying the maintenance and release of seasonal dormancy in peach. In order to achieve this we first used suppression subtractive hybridization (SSH) to identify genes expressed in dormant and dormancy-released buds in two cultivars with different chilling requirements, 'Zincal-5' and 'Springlady', and subsequently validated their differential expression utilizing a peach cDNA microarray platform containing transcripts enriched in flower buds. Additionally, we carried out a genome-wide search of peach genes related to dormancy release by hybridizing the previous cDNA microarray with mRNA samples from 10 cultivars showing different dormancy behaviour, followed by an expression correlation analysis.Among the most relevant genes identified in these two first works, we found the DORMANCY ASSOCIATED MADS-box genes DAM4, DAM5 and DAM6, described independently by other groups working in peach and other species. The central role of DAM genes in dormancy regulation has also been confirmed by additional functional approaches as the analysis of the non-dormant evg mutant, QTL analysis, and transgenic approaches.In our second work we focused on the molecular mechanisms of DAM6 down-regulation concomitant with dormancy release in flower buds. A ChIP analysis of DAM6 promoter and structural gene revealed chromatin modification events similar to those observed in vernalization of Arabidopsis and cereals. We showed that DAM6 is transcriptionally active in dormant buds collected in October, when a short chromatin region around its ATG was trimethylated in histone H3 at K4 (H3K4) and acetylated at the N-terminal tail of H3. Concomitantly with DAM6 repression, H3K4 became demethylated and H3 deacetylated. Later H3K27 was found trimethylated along a genomic region larger than 4kb, including promoter, coding sequence and intron. Due to their relevance in dormancy regulation, DAM genes could be used as expression markers to assess...

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Genetic linkage mapping for molecular dissection of chilling requirement and budbreak in apricot (Prunus armeniacaL.)

Cited by 85 publications

References 50 publications

Comparison of the genetic determinism of two key phenological traits, flowering and maturity dates, in three Prunus species: peach, apricot and sweet cherry

Comparison of the genetic determinism of two key phenological traits, flowering and maturity dates, in three Prunus species: peach, apricot and sweet cherry

Regulation of Bud Dormancy and Bud Break in Japanese Apricot (Prunus mume Siebold ^|^amp; Zucc.) and Peach [Prunus persica (L.) Batsch]: A Summary of Recent Studies

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

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