Genetic Linkage Mapping of RAPD Markers Segregating in Korean Ogol Chicken - White Leghorn Backcross Population

Hwang, Kyu Choon; Song, Kihwan; Kim, T.H.; Jeong, Dalsang; Sohn, Saejung; Lillehoj, H. S.; Han, Jae Yong

doi:10.5713/ajas.2001.302

Cited by 5 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The essence for the study was for stock analysis, taxonomy/systematic as well as selective breeding programmes (Waldman and Wirgin, 1993;Ward and Grewe, 1994;Carvalho and Hauser, 1994;Ferguson and Dangamam, 1998). Of the molecular markers used, Random Amplified Polymorphic DNA (RAPD) has been applied for the study of genetic diversity in several organisms including fish (Cagigas et al, 1999;Bartish et al, 2000;Lumaret et al, 2000;Mohd-Azmi et al, 2000;Hwang et al, 2001). This marker is a dominant marker and according to Theodorakis and Bickham (2004), it can sample a large number of loci and does not require the prior DNA sequence information to perform the assay.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the Genetic Diversity in Clarias gariepinus (Burchell, 1822) Using Random Amplified Polymorphic DNA (RAPD) Fingerprinting Technique

Ikpeme¹,

Udensi²,

Ekaluo³

et al. 2015

Asian J. of Animal Sciences

View full text Add to dashboard Cite

The utilization of molecular markers-assisted genetic diversity study is critical and imperative for a more robust selection and breeding programmes in aquaculture. Twenty C. gariepinus species were randomly selected, 5 each from four Rivers/locations-Okurikan (OKU), Lemna (LMN), UNICAL Fish Farm (UFF) and MCC cultured fish site, in Cross River State, Nigeria for genetic diversity study using RAPD technique. Genomic DNA extraction and purification from caudal fin tissue sample was performed using ZR Genomic DNA tissue extraction Kit, primed using OPA09, OPA11 and OPA13 RAPD primers and amplified using PCR. The amplified fragments were ran on gel electrophoresis and visualized under ethidium bromide dye. Thirty four bands were generated by the three RAPD primers from the four C. gariepinus populations of which 28 were polymorphic, accounting for 82.35%. Polymorphism revealed by each fish population ranged from 92.86-100% while, gene diversity estimates ranged from 0.246±0.16-0.385±0.09. Intra-population similarity index ranged from 42.49-78.4% while, the inter-population similarity index across all population combinations ranged from 52.32-71.69%. These result also revealed that as genetic distances increases, genetic identities narrows between the wild and the cultured species. UPGMA dendrogram generated two major clusters with C. gariepinus species from OKU, UFF and MCC in cluster-1 and C. gariepinus species from LMN in cluster-2. Implicitly though, our results suggest that there were several genetic diversity in the populations studied, especially between the cultured and the wild, which could encourage selection of C. gariepinus from OKU and LMN populations for the purpose of breeding and improvement.

show abstract

Section: Introductionmentioning

confidence: 99%

Unveiling the Genetic Diversity in Clarias gariepinus (Burchell, 1822) Using Random Amplified Polymorphic DNA (RAPD) Fingerprinting Technique

Ikpeme¹,

Udensi²,

Ekaluo³

et al. 2015

Asian J. of Animal Sciences

View full text Add to dashboard Cite

show abstract

“…have been so far used to analyze genetic similarity and diversity in genetics and breeding research of fish/invertebrates. Also, molecular markers from random amplified polymorphic DNA (RAPD) have recently been used to evaluate genetic diversity and/or similarity in several organisms (Cagigas et al, 1999;Bartish et al, 2000;Huang et al, 2000;Lumaret al, 2000;Mohd-Azmi et al, 2000;Hwang et al, 2001). Particularly, markers from 1,357 individuals from 47 populations collected at three sampling dates for 16 sites were used to measure genetic diversity within and among populations (Mengistu et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic Similarity and Variation in the Cultured and Wild Crucian Carp (Carassius carassius) Estimated with Random Amplified Polymorphic DNA

Yoon¹,

Park²

2002

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

Random amplified polymorphic DNA (RAPD) analysis based on numerous polymorphic bands have been used to investigate genetic similarity and diversity among and within two cultured and wild populations represented by the species crucian carp (Carassius carassius). From RAPD analysis using five primers, a total of 442 polymorphic bands were obtained in the two populations and 273 were found to be specific to a wild population. 169 polymorphic bands were also produced in wild and cultured population. According to RAPD-based estimates, the average number of polymorphic bands in the wild population was approximately 1.5 times as diverse as that in cultured. The average number of polymorphic bands in each population was found to be different and was higher in the wild than in the cultured population. Comparison of banding patterns in the cultured and wild populations revealed substantial differences supporting a previous assessment that the populations may have been subjected to a long period of geographical isolation from each other. The values in wild population altered from 0.21 to 0.51 as calculated by bandsharing analysis. Also, the average level of bandsharing values was 0.40±0.05 in the wild population, compared to 0.69±0.08 in the cultured. With reference to bandsharing values and banding patterns, the wild population was considerably more diverse than the cultured. Knowledge of the genetic diversity of crucian carp could help in formulating more effective strategies for managing this aquacultural fish species and also in evaluating the potential genetic effects induced by hatchery operations.

show abstract

“…Several chemical and biochemical techniques such as Iso-zyme electrophoresis (Smith et al, 1997;Cagigas et al, 1999), restriction fragment length polymorphism (RFLP) (Hallerman and Beckmann, 1988) and microsatellites (Huang et al, 2000) have been used in analyzing genetic similarity and diversity in genetics and breeding research involving fish and other vertebrates. Random amplified polymorphic DNA (RAPD) has been used to evaluate genetic diversity and similarity in several organisms (Cagigas et al, 1999;Bartish et al, 2000;Huang et al, 2000;Lumaret et al, 2000;Mohd-Azmi et al, 2000;Hwang et al, 2001). Application of RAPD in the study of genetic similarity and diversity was reported in fish (Coughlan et al, 1998;Cagigas et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Popoola

Fasakin

Awopetu

2014

CJF

View full text Add to dashboard Cite

Three wild populations of Clarias gariepinus from Esaodo (River Osun), Owena (River Owena), and Agbabu (River Oluwa), and three farmed populations, viz: Akure, Ilesa and Ado-Ekiti, in Southwest Nigeria, were analysed for their genetic differences using Random amplified polymorphic DNA (RAPD) analysis. Live specimens comprising 40 individuals (680 ± 3.28 g) from each location were collected and kept in six concrete tanks (2x1x1m). Altogether 435 reproducible bands were obtained from six populations for the nine RAPD primers used. The Analysis of Molecular Variance (AMOVA) indicated that the sampled populations are significantly different from each other, and that 99% of the total variation resided within the population. The percentage of genetic identity (GI) of RAPD-PCR profile among six populations ranged from 74.6% to 83.5%, while Genetic distance of the six populations based on RAPD-PCR profile ranged from 0.180 to 0.293. Estimates of genetic variation in wild and cultured populations of C. gariepinus were made, and total and mean number of segregating fragments were 71 (89.9%), 34.5 and 59 (74.7%), 35.4, respectively. Total gene diversity within wild and cultured populations (Ht) was 0.3419 and 0.3010, respectively. The study established that there is genetic variability in both wild and cultured C. gariepinus. RAPD showed that samples within the wild and cultured populations under study were closer to each other than between the two habitats. With reference to total gene diversity values and total number of segregating fragments, the wild population was considerably more diverse than the cultured population.

show abstract

Genetic Linkage Mapping of RAPD Markers Segregating in Korean Ogol Chicken - White Leghorn Backcross Population

Cited by 5 publications

References 0 publications

Unveiling the Genetic Diversity in Clarias gariepinus (Burchell, 1822) Using Random Amplified Polymorphic DNA (RAPD) Fingerprinting Technique

Unveiling the Genetic Diversity in Clarias gariepinus (Burchell, 1822) Using Random Amplified Polymorphic DNA (RAPD) Fingerprinting Technique

Genetic Similarity and Variation in the Cultured and Wild Crucian Carp (Carassius carassius) Estimated with Random Amplified Polymorphic DNA

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Contact Info

Product

Resources

About