Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.) compared with the almond Texas × peach Earlygold reference map for Prunus

Abstract

Several genetic linkage maps have been published in recent years on different Prunus species suggesting a high level of resemblance among the genomes of these species. One of these maps (Joobeur et al., Theor Appl Genet 97:1034-1041 [(1998); Aranzana et al., Theor Appl Genet 106:819-825 (2002b)] constructed from interspecific almond Texas x peach Earlygold F(2) progeny (TxE) was considered to be saturated. We selected 142 F(1) apricot hybrids obtained from a cross between P. armeniaca cvs. Polonais and Stark Early Orange for mapping. Eighty-eight RFLP probes and 20 peach SSR primer pairs used for the 'reference map' were selected to cover the eight linkage groups. One P. davidiana and an additional 14 apricot simple sequence repeats (SSRs) were mapped for the F(1) progeny. Eighty-three amplified fragment length polymorphisms were added in order to increase the density of the maps. Separate maps were made for each parent according to the 'double pseudo-testcross' model of analysis. A total of 141 markers were placed on the map of Stark Early Orange, defining a total length of 699 cM, and 110 markers were placed on the map of Polonais, defining a total length of 538 cM. Twenty-one SSRs and 18 restriction placed in the TxE map were heterozygous in both parents (anchor loci), thereby enabling the alignment of the eight homologous linkage groups of each map. Except for 15 markers, most markers present in each linkage group in apricot were aligned with those in TxE map, indicating a high degree of colinearity between the apricot genome and the peach and almond genomes. These results suggest a strong homology of the genomes between these species and probably between Prunophora and Amygdalus sub-genera.

Genetic diversity in fruiting and flower-ornamental Japanese apricot (Prunus mume) germplasms assessed by SSR markers

“…Gao et al (2004) reported 1 to 18 alleles per locus for 24 Japanese apricot cultivars in China by SSR analysis, with an average value of 9.1. One to 9 alleles were obtained for 14 peach cultivars (Yamamoto et al 2002) and 3 to 12 alleles were identified for SSR analysis of 25 apricot cultivars (Lambert et al 2004). In flowering cherry, the number of alleles per locus ranged from 4 to 36 by SSR analysis of 144 cultivars (Ohta et al 2005).…”

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

“…Genomic co-linearity and marker transferability has been demonstrated within members of the Rosaceae (Joobeur et al, 1998;Dirlewanger et al, 2004aDirlewanger et al, , 2004bLambert et al, 2004;Dondini et al, 2007;Olmstead et al, 2008;Clarke et al, 2009;Cabrera et al, 2009). In 2009, a set of onserved Rosaceae genebased sequences corresponding to single copy Arabidopsis genes was developed (Cabrera et al, 2009).…”

Genetic variability among local apricots (Prunus armeniaca L.) from the Southeast of Spain

“…Many of these genetic markers have been developed along the years, and from an early phase many of them have been applied to the genus Prunus, starting with the construction of a genetic map for improving breeding selection in peach (Chaparro et al, 1994). At least three genetic linkage maps of apricot have been published already (Hurtado et al, 2002;Vilanova et al, 2003;Lambert et al, 2004). Apricot diversity and genetic relationships have been studied using isozymes (Byrne and Littleton, 1989;Badenes et al, 1996), restriction fragment length polymorphisms (RFLPs) (De Vicente et al, 1998), random amplified polymorphic DNA (RAPDs) (Badenes et al, 2000), amplified fragment length polymorphisms (AFLPs) (Hagen et al, 2002;Hurtado et al, 2002) and sequence characterised amplified regions (SCARs) (Mariniello et al, 2002).…”