Genetic Logic Gates Enable Patterning of Amyloid Nanofibers

Kalyoncu, Ebuzer; Ahan, Recep Erdem; Özçelik, Cemile Elif; Şeker, Urartu Özgür Şafak

doi:10.1002/adma.201902888

Cited by 24 publications

(24 citation statements)

References 37 publications

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“…Not surprisingly, the majority of ELMs rely on materials natively produced by the host organism. For example, several amyloid-based materials have been synthesized by genetically tractable bacteria, such as aggregates of CsgA in Escherichia coli (11,12) and TasA in Bacillus subtilis (13). Genetic fusions have allowed these fibrous matrices to bind specific molecules, conduct electricity, perform catalytic reactions, and adhere to complex surfaces (14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Genetic Control of Radical Cross-linking in a Semisynthetic Hydrogel

Graham

Dundas

Hillsley

et al. 2020

ACS Biomater. Sci. Eng.

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Enhancing materials with the qualities of living systems, including sensing, computation, and adaptation, is an important challenge in designing next-generation technologies. Living materials seek to address this challenge by incorporating live cells as actuating components that control material function. For abiotic materials, this requires new methods that couple genetic and metabolic processes to material properties. Toward this goal, we demonstrate that extracellular electron transfer (EET) from Shewanella oneidensis can be leveraged to control radical crosslinking of a methacrylate-functionalized hyaluronic acid hydrogel. Crosslinking rates and hydrogel mechanics, specifically storage modulus, were dependent on a variety of chemical and biological factors, including S. oneidensis genotype. Bacteria remained viable and metabolically active in the crosslinked network for a least one week, while cell tracking revealed that EET genes also encode control over hydrogel microstructure. Moreover, construction of an inducible gene circuit allowed transcriptional control of storage modulus and crosslinking rate via the tailored expression of a key electron transfer protein, MtrC. Finally, we quantitatively modeled dependence of hydrogel stiffness on steady-state gene expression, and generalized this result by demonstrating the strong relationship between relative gene expression and material properties. This general mechanism for radical crosslinking provides a foundation for programming the form and function of synthetic materials through genetic control over extracellular electron transfer.

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Section: Introductionmentioning

confidence: 99%

Genetic Control of Radical Cross-linking in a Semisynthetic Hydrogel

Graham

Dundas

Hillsley

et al. 2020

ACS Biomater. Sci. Eng.

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“…To generate biofilms with adjustable properties, the expression rates of different CsgA variants can be tuned. For example, the Seker group engineered an E. coli strain with two expression cassettes, one for wildtype CsgA and one for His-tagged CsgA [ 206 ]. The corresponding promoters were placed between recombination sites so that the orientation of each promoter could be inverted by inducing the expression of a corresponding recombinase.…”

Section: Elmsmentioning

confidence: 99%

Synthetic biology as driver for the biologization of materials sciences

Burgos-Morales

Gueye

Lacombe

et al. 2021

Materials Today Bio

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Materials in nature have fascinating properties that serve as a continuous source of inspiration for materials scientists. Accordingly, bio-mimetic and bio-inspired approaches have yielded remarkable structural and functional materials for a plethora of applications. Despite these advances, many properties of natural materials remain challenging or yet impossible to incorporate into synthetic materials. Natural materials are produced by living cells, which sense and process environmental cues and conditions by means of signaling and genetic programs, thereby controlling the biosynthesis, remodeling, functionalization, or degradation of the natural material. In this context, synthetic biology offers unique opportunities in materials sciences by providing direct access to the rational engineering of how a cell senses and processes environmental information and translates them into the properties and functions of materials. Here, we identify and review two main directions by which synthetic biology can be harnessed to provide new impulses for the biologization of the materials sciences: first, the engineering of cells to produce precursors for the subsequent synthesis of materials. This includes materials that are otherwise produced from petrochemical resources, but also materials where the bio-produced substances contribute unique properties and functions not existing in traditional materials. Second, engineered living materials that are formed or assembled by cells or in which cells contribute specific functions while remaining an integral part of the living composite material. We finally provide a perspective of future scientific directions of this promising area of research and discuss science policy that would be required to support research and development in this field.

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“…The resulting fluorescent silks exhibit similar strain and Young's modulus to those of ordinary silk. The excellent mechanical properties of protein‐based fibers showed their versatile potential applications in the areas of biophysics, biomedical engineering, bioelectronics, and materials science …”

Section: Introductionmentioning

confidence: 99%

Fabrication and Mechanical Properties of Engineered Protein‐Based Adhesives and Fibers

Sun

et al. 2019

Advanced Materials

124

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Protein-based structural biomaterials are of great interest for various applications because the sequence flexibility within the proteins may result in their improved mechanical and structural integrity and tunability. As the two representative examples, protein-based adhesives and fibers have attracted tremendous attention. The typical protein adhesives, which are secreted by mussels, sandcastle worms, barnacles, and caddisfly larvae, exhibit robust underwater adhesion performance. In order to mimic the adhesion performance of these marine organisms, two main biological adhesives are presented, including genetically engineered protein-based adhesives and biomimetic chemically synthetized adhesives. Moreover, various protein-based fibers inspired by spider and silkworm proteins, collagen, elastin, and resilin are studied extensively. The achievements in synthesis and fabrication of structural biomaterials by DNA recombinant technology and chemical regeneration certainly will accelerate the explorations and applications of protein-based adhesives and fibers in wound healing, tissue regeneration, drug delivery, biosensors, and other high-tech applications. However, the mechanical properties of the biological structural materials still do not match those of natural systems. More efforts need to be devoted to the study of the interplay of the protein structure, cohesion and adhesion effects, fiber processing, and mechanical performance.

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Genetic Logic Gates Enable Patterning of Amyloid Nanofibers

Cited by 24 publications

References 37 publications

Genetic Control of Radical Cross-linking in a Semisynthetic Hydrogel

Genetic Control of Radical Cross-linking in a Semisynthetic Hydrogel

Synthetic biology as driver for the biologization of materials sciences

Fabrication and Mechanical Properties of Engineered Protein‐Based Adhesives and Fibers

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