2018
DOI: 10.1016/j.biortech.2018.08.024
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Genetic manipulation of Escherichia coli central carbon metabolism for efficient production of fumaric acid

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Cited by 28 publications
(19 citation statements)
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“…Metabolic engineering is conducted to rewire the complete noncyclic glyoxylate pathway for fumarate production. Recently, five metabolic engineering strategies have been developed to enhance production of fumarate: reconstructing synthetic pathway, such as the reductive TCA cycle [ 2 ], the oxidative TCA cycle [ 3 ], the noncyclic glyoxylate cycle [ 4 ], the urea cycle and the purine nucleotide cycle [ 27 , 28 ]; eliminating byproducts formation [ 27 ], such as lactate, acetate, formate, malate, and succinate; optimizing oxidation and reduction levels [ 5 ]; modifying glucose transport system [ 8 ]; regulating C 4 -dicarboxylate transporter [ 26 ]. These results indicated that fumarate production has been improved by metabolic engineering strategies.…”
Section: Discussionmentioning
confidence: 99%
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“…Metabolic engineering is conducted to rewire the complete noncyclic glyoxylate pathway for fumarate production. Recently, five metabolic engineering strategies have been developed to enhance production of fumarate: reconstructing synthetic pathway, such as the reductive TCA cycle [ 2 ], the oxidative TCA cycle [ 3 ], the noncyclic glyoxylate cycle [ 4 ], the urea cycle and the purine nucleotide cycle [ 27 , 28 ]; eliminating byproducts formation [ 27 ], such as lactate, acetate, formate, malate, and succinate; optimizing oxidation and reduction levels [ 5 ]; modifying glucose transport system [ 8 ]; regulating C 4 -dicarboxylate transporter [ 26 ]. These results indicated that fumarate production has been improved by metabolic engineering strategies.…”
Section: Discussionmentioning
confidence: 99%
“…Pathway optimization represents one significant step in identifying and removing the potential bottlenecks to improve the transmission efficiency of biosynthetic pathway. To improve the transmission efficiency of intermediate metabolites, the partial noncyclic glyoxylate pathway was constructed by replacing the native PEP-dependent PTSG system with the PEP-independent galactose translocation system, overexpressing phosphoenolpyruvate carboxylase (PPC) and acetyl-CoA synthase, and deleting malate dehydrogenase, fumarate reductase, and fumarase [ 8 ]. The final concentration of fumarate (1.53 g/g dry cell weight) was increased by 50% compared with the parental strain.…”
Section: Discussionmentioning
confidence: 99%
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“…A high-performance liquid chromatograph (HPLC; Shimadzu Corporation, Kyoto, Japan) equipped with a refractive index detector and UV detector (210 nm) was utilized to detect the titer of fumaric acid, by-product ethanol, and glucose consumption. The column used was a Bio-Rad Aminex HPX-87 H ion exclusion column (Richmond, CA, USA), and 0.005 M H2SO4 was used as eluent at 50 ℃ with a flow rate of 0.6 mL/min (Liu et al 2018a). Due to the low solubility of fumaric acid and calcium fumarate, both accumulated as precipitated in the broth.…”
Section: Methodsmentioning
confidence: 99%
“…This strategy helped to achieve the best yield (1.53 g/g dcw) is 50% higher compared to the parental strain. For the modifications in E. Coli , PEP‐dependent glucose phosphotransferase enzyme groups were replaced by a galactic translocation system that reduced consumption of PEP as intermediates [76].…”
Section: Primary Metabolitesmentioning
confidence: 99%