Genetic Manipulation of Quinone Biosynthesis in Cyanobacteria

Sakuragi, Yumiko; Bryant, Donald A.

doi:10.1007/978-1-4020-4256-0_15

Cited by 17 publications

(17 citation statements)

References 102 publications

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“…On the basis of sequence analysis, which has shown that homologues of genes encoding five enzymes participating in ubiquinone-8 (UQ-8) biosynthesis in E. coli (UbiA, UbiD, UbiE, UbiH, UbiX) are present in 14 cyanobacterial genomes, Sakuragi and Bryant proposed that PQ biosynthetic pathway in cyanobacteria resembles that of UQ in a-, band c-proteobacteria (Fig. 2) (Sakuragi and Bryant 2006).…”

Section: Introductionmentioning

confidence: 99%

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Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Nowicka

Kruk

2016

Acta Physiol Plant

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Section: Introductionmentioning

confidence: 99%

“…PCC 6803, there is a need to explain these differences. The other interesting question is (Sakuragi and Bryant 2006 …”

Section: Introductionmentioning

confidence: 99%

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Nowicka

Kruk

2016

Acta Physiol Plant

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“…a-Tocopherol (vitamin E) is a lipid-soluble, organic molecule that is only synthesized by oxygen-evolving phototrophs, including some cyanobacteria and all green algae and plants (Threlfall and Whistance, 1971;Collins and Jones, 1981;Sakuragi and Bryant, 2006). The conservation of a-tocopherol synthesis during the evolution of oxygenic photosynthetic organisms suggests that this molecule performs one or more critical functions.…”

mentioning

confidence: 99%

α-Tocopherol Plays a Role in Photosynthesis and Macronutrient Homeostasis of the Cyanobacterium Synechocystis sp. PCC 6803 That Is Independent of Its Antioxidant Function

et al. 2006

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a-Tocopherol is synthesized exclusively in oxygenic phototrophs and is known to function as a lipid-soluble antioxidant. Here, we report that a-tocopherol also has a novel function independent of its antioxidant properties in the cyanobacterium Synechocystis sp. PCC 6803. The photoautotrophic growth rates of wild type and mutants impaired in a-tocopherol biosynthesis are identical, but the mutants exhibit elevated photosynthetic activities and glycogen levels. When grown photomixotrophically with glucose (Glc), however, these mutants cease growth within 24 h and exhibit a global macronutrient starvation response associated with nitrogen, sulfur, and carbon, as shown by decreased phycobiliprotein content (35% of the wild-type level) and accumulation of the nblA1-nblA2, sbpA, sigB, sigE, and sigH transcripts. Photosystem II activity and carboxysome synthesis are lost in the tocopherol mutants within 24 h of photomixotrophic growth, and the abundance of carboxysome gene (rbcL, ccmK1, ccmL) and ndhF4 transcripts decreases to undetectable levels. These results suggest that a-tocopherol plays an important role in optimizing photosynthetic activity and macronutrient homeostasis in Synechocystis sp. PCC 6803. Several lines of evidence indicate that increased oxidative stress in the tocopherol mutants is unlikely to be the underlying cause of photosystem II inactivation and Glcinduced lethality. Interestingly, insertional inactivation of the pmgA gene, which encodes a putative serine-threonine kinase similar to RsbW and RsbT in Bacillus subtilis, results in a similar increase in glycogen and Glc-induced lethality. Based on these results, we propose that a-tocopherol plays a nonantioxidant regulatory role in photosynthesis and macronutrient homeostasis through a signal transduction pathway that also involves PmgA.

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“…1). Phylloquinone is synthesized by plants and most cyanobacteria (1), whereas menaquinone is synthesized by facultative anaerobic bacteria, archaea, and some cyanobacteria (1,2). Vitamin K plays very different roles in the organisms that synthesize it compared to those that do not.…”

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confidence: 99%

“…The subsequent removal of CoA is of special interest, for it frees the carboxyl group of DHNA and allows its conjugation to the polyisoprenyl chain for anchoring into biological membranes. The hydrolysis of DHNA-CoA has long been a puzzling mystery of vitamin K biosynthesis, and it is actually not clear whether this step is enzyme-driven or a purely chemical process (1). It was first thought to occur concomitantly to the cyclization step catalyzed by DHNA synthase, hence the name of this enzyme (17).…”

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confidence: 99%

A dedicated thioesterase of the Hotdog-fold family is required for the biosynthesis of the naphthoquinone ring of vitamin K ₁

Widhalm

Oostende

Furt

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Phylloquinone (vitamin K1) is a bipartite molecule that consists of a naphthoquinone ring attached to a phytyl side chain. The coupling of these 2 moieties depends on the hydrolysis of the CoA thioester of 1,4-dihydroxy-2-naphthoate (DHNA), which forms the naphthalenoid backbone. It is not known whether such a hydrolysis is enzymatic or chemical. In this study, comparative genomic analyses identified orthologous genes of unknown function that in most species of cyanobacteria cluster with predicted phylloquinone biosynthetic genes. The encoded approximately 16-kDa proteins display homology with some Hotdog domain-containing CoA thioesterases that are involved in the catabolism of 4-hydroxybenzoyl-CoA and gentisyl-CoA (2,5-dihydroxybenzoyl-CoA) in certain soil-dwelling bacteria. The Synechocystis ortholog, encoded by gene slr0204, was expressed as a recombinant protein and was found to form DHNA as reaction product. Unlike its homologs in the Hotdog domain family, Slr0204 showed strict substrate specificity. The Synechocystis slr0204 knockout was devoid of DHNA-CoA thioesterease activity and accumulated DHNA-CoA. As a result, knockout cells contained 13-fold less phylloquinone than their wild-type counterparts and displayed the typical photosensitivity to high light associated to phylloquinone deficiency in cyanobacteria.cyanobacteria ͉ isoprenoids ͉ photosynthesis ͉ phylloquinone

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Genetic Manipulation of Quinone Biosynthesis in Cyanobacteria

Cited by 17 publications

References 102 publications

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

α-Tocopherol Plays a Role in Photosynthesis and Macronutrient Homeostasis of the Cyanobacterium Synechocystis sp. PCC 6803 That Is Independent of Its Antioxidant Function

A dedicated thioesterase of the Hotdog-fold family is required for the biosynthesis of the naphthoquinone ring of vitamin K ₁

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Genetic Manipulation of Quinone Biosynthesis in Cyanobacteria

Cited by 17 publications

References 102 publications

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

α-Tocopherol Plays a Role in Photosynthesis and Macronutrient Homeostasis of the Cyanobacterium Synechocystis sp. PCC 6803 That Is Independent of Its Antioxidant Function

A dedicated thioesterase of the Hotdog-fold family is required for the biosynthesis of the naphthoquinone ring of vitamin K 1

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A dedicated thioesterase of the Hotdog-fold family is required for the biosynthesis of the naphthoquinone ring of vitamin K ₁