Genetic Manipulation of the Mouse Developing Hypothalamus through &lt;em&gt;In utero&lt;/em&gt; Electroporation

Haddad-Tóvolli, Roberta; Szabó, Nora-Emöke; Zhou, Xunlei; Álvarez-Bolado, Gonzalo

doi:10.3791/50412-v

Cited by 3 publications

(2 citation statements)

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“…STAligner can also detect small (proportion<5%) structures, such as hypothalamus and olfactory bulb, at later developmental stages (after E11.5), which are supported by known histological evidence. The hypothalamic neurogenesis starts at E11.5 and reaches the peak at E12.5 27 . The development of olfactory bulb initiated at E10.5 and cannot be detected macroscopically until E12.5 28,29 .…”

Section: Staligner Reveals Detailed Developmental Dynamics Of Anatomi...mentioning

confidence: 99%

Integrating spatial transcriptomics data across different conditions, technologies and developmental stages

Zhou,

Dong,

Zhang

2023

Nat Comput Sci

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With the rapid generation of spatial transcriptomics (ST) data, integrative analysis of multiple ST datasets from different conditions, technologies, and developmental stages is becoming increasingly important. However, identifying shared and specific spatial domains across ST datasets of multiple slices remains challenging. To this end, we develop a graph attention neural network STAligner for integrating and aligning ST datasets, enabling spatially-aware data integration, simultaneous spatial domain identification, and downstream comparative analysis. We apply STAligner to the integrative analysis of ST datasets of the human cortex slices from different samples, the mouse olfactory bulb slices generated by two profiling technologies, the mouse hippocampus tissue slices under normal and Alzheimer's disease conditions, and the spatiotemporal atlases of mouse organogenesis. STAligner efficiently captures the shared tissue structures across different slices, the disease-related substructures, and the dynamical changes during mouse embryonic development. Additionally, the shared spatial domain and nearest neighbor pairs identified by STAligner can be further considered as corresponding pairs to guide the threedimensional reconstruction of consecutive slices, achieving more accurate local structure-guided registration results than the existing method.

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Section: Staligner Reveals Detailed Developmental Dynamics Of Anatomi...mentioning

confidence: 99%

Integrating spatial transcriptomics data across different conditions, technologies and developmental stages

Zhou,

Dong,

Zhang

2023

Nat Comput Sci

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“…Here, we briefly review how to perform IUE and the underlying mechanism. Though IUE has also been successfully applied to ferrets (Kawasaki et al., 2012) and has been used to target brain regions other than the cerebral cortex (Bonnin et al., 2007; Haddad‐Tovolli et al., 2013), we will focus this review on the applications of IUE on rodents (viz., mice and rats) to study the development of the cerebral cortex. We discuss both classical and novel approaches to IUE, including transfection of plasmid DNA, RNA interference (RNAi), CRISPR/CAS9 genome editing, transposase‐mediated recombination, and other novel approaches.…”

Section: Introductionmentioning

confidence: 99%

Advances in inutero electroporation

Kittock

Pilaz

2023

Developmental Neurobiology

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In utero electroporation (IUE) is a technique developed in the early 2000s to transfect the neurons and neural progenitors of embryonic brains, thus enabling continued development in utero and subsequent analyses of neural development. Early IUE experiments focused on ectopic expression of plasmid DNA to analyze parameters such as neuron morphology and migration. Recent advances made in other fields, such as CRISPR/CAS9 genome editing, have been incorporated into IUE techniques as they were developed. Here, we provide a general review of the mechanics and techniques involved in IUE and explore the breadth of approaches that can be used in conjunction with IUE to study cortical development in a rodent model, with a focus on the novel advances in IUE techniques. We also highlight a few cases that exemplify the potential of IUE to study a broad range of questions in neural development.

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Temporal-spatial Generation of Astrocytes in the Developing Diencephalon

Hong,

Gong,

Pan

et al. 2023

Neurosci. Bull.

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Astrocytes are the largest glial population in the mammalian brain. However, we have a minimal understanding of astrocyte development, especially fate specification in different regions of the brain. Through lineage tracing of the progenitors of the third ventricle (3V) wall via in-utero electroporation in the embryonic mouse brain, we show the fate specification and migration pattern of astrocytes derived from radial glia along the 3V wall. Unexpectedly, radial glia located in different regions along the 3V wall of the diencephalon produce distinct cell types: radial glia in the upper region produce astrocytes and those in the lower region produce neurons in the diencephalon. With genetic fate mapping analysis, we reveal that the first population of astrocytes appears along the zona incerta in the diencephalon. Astrogenesis occurs at an early time point in the dorsal region relative to that in the ventral region of the developing diencephalon. With transcriptomic analysis of the region-specific 3V wall and lateral ventricle (LV) wall, we identified cohorts of differentially-expressed genes in the dorsal 3V wall compared to the ventral 3V wall and LV wall that may regulate astrogenesis in the dorsal diencephalon. Together, these results demonstrate that the generation of astrocytes shows a spatiotemporal pattern in the developing mouse diencephalon.

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Genetic Manipulation of the Mouse Developing Hypothalamus through <em>In utero</em> Electroporation

Cited by 3 publications

References 0 publications

Integrating spatial transcriptomics data across different conditions, technologies and developmental stages

Integrating spatial transcriptomics data across different conditions, technologies and developmental stages

Advances in inutero electroporation

Temporal-spatial Generation of Astrocytes in the Developing Diencephalon

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