Genetic manipulation of γ‐linolenic acid (GLA) synthesis in a commercial variety of evening primrose (<i>Oenothera</i> sp.)

Gyves, Emilio Mendoza de; Sparks, Caroline A.; Sayanova, Olga; Lazzeri, P. A.; Napier, Johnathan A.; Jones, Huw

doi:10.1111/j.1467-7652.2004.00079.x

Cited by 23 publications

(7 citation statements)

References 32 publications

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“…Consistent with this, the transcript levels of both the CSD8 and CSD6 are very low from the T to U stages of embryo development and are not detected at the FND stage (Figure a). Up‐regulation of CSD6 may therefore be the best strategy to increase amounts of GLA as has been demonstrated by genetic engineering of tobacco (Reddy and Thomas, ; Sayanova et al ., ), Brassica juncea (Hong et al ., ), Brassica napus (Liu et al ., ) and evening primrose (de Gyves et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Targeted mutation of Δ12 and Δ15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil

Bielecka

Kaminski

Adams

et al. 2014

Plant Biotechnology Journal

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SummaryWe used expressed sequence tag library and whole genome sequence mining to identify a suite of putative desaturase genes representing the four main activities required for production of polyunsaturated fatty acids in hemp seed oil. Phylogenetic-based classification and developing seed transcriptome analysis informed selection for further analysis of one of seven D12 desaturases and one of three D15 desaturases that we designate CSFAD2A and CSFAD3A, respectively. Heterologous expression of corresponding cDNAs in Saccharomyces cerevisiae showed CSFAD2A to have Dx+3 activity, while CSFAD3A activity was exclusively at the D15 position. TILLING of an ethyl methane sulphonate mutagenized population identified multiple alleles including non-sense mutations in both genes and fatty acid composition of seed oil confirmed these to be the major D12 and D15 desaturases in developing hemp seed. Following four backcrosses and sibling crosses to achieve homozygosity, csfad2a-1 was grown in the field and found to produce a 70 molar per cent high oleic acid (18:1 D9 ) oil at yields similar to wild type. Cold-pressed high oleic oil produced fewer volatiles and had a sevenfold increase in shelf life compared to wild type. Two low abundance octadecadienoic acids, 18:2 D6,9 and 18:2 D9,15 , were identified in the high oleic oil, and their presence suggests remaining endogenous desaturase activities utilize the increased levels of oleic acid as substrate. Consistent with this, CSFAD3A produces 18:2 D9,15 from endogenous 18:1 D9 when expressed in S. cerevisiae. This work lays the foundation for the development of additional novel oil varieties in this multipurpose low input crop.

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Section: Discussionmentioning

confidence: 97%

Targeted mutation of Δ12 and Δ15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil

Bielecka

Kaminski

Adams

et al. 2014

Plant Biotechnology Journal

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“…Furthermore, Oenothera tissue culture has been applied for industrial production of pharmaceutically active secondary metabolites (Taniguchiet al 2002). Consequently, the genus is of interest for commercial breeding and crop improvement by genetic manipulation (De Gyves et al 2004;Fieldsend 2007).…”

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confidence: 99%

Molecular Marker Systems for Oenothera Genetics

et al. 2008

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The genus Oenothera has an outstanding scientific tradition. It has been a model for studying aspects of chromosome evolution and speciation, including the impact of plastid nuclear co-evolution. A large collection of strains analyzed during a century of experimental work and unique genetic possibilities allow the exchange of genetically definable plastids, individual or multiple chromosomes, and/or entire haploid genomes (Renner complexes) between species. However, molecular genetic approaches for the genus are largely lacking. In this study, we describe the development of efficient PCR-based marker systems for both the nuclear genome and the plastome. They allow distinguishing individual chromosomes, Renner complexes, plastomes, and subplastomes. We demonstrate their application by monitoring interspecific exchanges of genomes, chromosome pairs, and/or plastids during crossing programs, e.g., to produce plastome-genome incompatible hybrids. Using an appropriate partial permanent translocation heterozygous hybrid, linkage group 7 of the molecular map could be assigned to chromosome 9Á8 of the classical Oenothera map. Finally, we provide the first direct molecular evidence that homologous recombination and free segregation of chromosomes in permanent translocation heterozygous strains is suppressed.

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“…GLA is a PUFA in the human body with many medicinal and health‐promoting effects; it can be used in cosmetics and medicine . The main sources of GLA are plant seeds and fungi from fermentation, with the type of fermentation (solid‐ versus submerged‐state fermentation) affecting the production of metabolites . However, it is obvious that GLA production from the current sources is inadequate for supplying the expanding market, due to such significant problems as low productivity, insecurity, expensive downstream processing, etc.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional characterization of a Δ6‐fatty acid desaturase gene from Rhizopus oryzae

Zhu

Zhang

2012

J. Basic Microbiol.

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The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology to fungal Δ6-fatty acid desaturase genes was isolated from R. oryzae by using the rapid amplification of cDNA ends method. It had an open reading frame of 1176 bp encoding a deduced polypeptide of 391 amino acids. Bioinformatics analysis characterized the putative RoD6D protein as a typical membrane-bound desaturase, including three conserved histidine-rich motifs, a hydropathy profile, and a cytochrome b5 -like domain in the N terminus. When the coding sequence was expressed in the Saccharomyces cerevisiae strain INVScl, the encoded product of RoD6D exhibited Δ6-fatty acid desaturase activity that led to the accumulation of γ-linolenic acid. The corresponding genomic sequence of RoD6D was 1565 bp in length, with five introns. This is the first report on the characterization and gene cloning of a Δ6-fatty acid desaturase of R. oryzae from Douchi.

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Genetic manipulation of γ‐linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

Cited by 23 publications

References 32 publications

Targeted mutation of Δ12 and Δ15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil

Targeted mutation of Δ12 and Δ15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil

Molecular Marker Systems for Oenothera Genetics

Molecular cloning and functional characterization of a Δ6‐fatty acid desaturase gene from Rhizopus oryzae

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