Genetic map of Saccharomyces cerevisiae.

Mortimer, Robert; Schild, David

doi:10.1128/mmbr.44.4.519-571.1980

Cited by 205 publications

(125 citation statements)

References 13 publications

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“…Comparison of the genetic and physical maps of yeast chromosome X. The genetic map is re-drawn from Mortimer (Mortimer et al, 1995). The unmapped genes or markers are listed on the right.…”

Section: Discussionmentioning

confidence: 99%

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Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.

et al. 1996

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The complete nucleotide sequence of Saccharomyces cerevisiae chromosome X (745 442 bp) reveals a total of 379 open reading frames (ORFs), the coding region covering approximately 75% of the entire sequence. One hundred and eighteen ORFs (31%) correspond to genes previously identified in S. cerevisiae. All other ORFs represent novel putative yeast genes, whose function will have to be determined experimentally. However, 57 of the latter subset (another 15% of the total) encode proteins that show significant analogy to proteins of known function from yeast or other organisms. The remaining ORFs, exhibiting no significant similarity to any known sequence, amount to 54% of the total. General features of chromosome X are also reported, with emphasis on the nucleotide frequency distribution in the environment of the ATG and stop codons, the possible coding capacity of at least some of the small ORFs (<100 codons) and the significance of 46 non‐canonical or unpaired nucleotides in the stems of some of the 24 tRNA genes recognized on this chromosome.

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“…Comparison of the genetic and physical maps of yeast chromosome X. The genetic map is re-drawn from Mortimer (Mortimer et al, 1995). The unmapped genes or markers are listed on the right.…”

Section: Discussionmentioning

confidence: 99%

“…Comparison of the physical and genetic maps of the chromosome X The genetic map of chromosome X includes 60 genes or markers, of which 48 were mapped in a linear array and 12 remained unmapped (Mortimer et al, 1995). Figure 7 shows a comparison of this map with the physical map deduced from the complete nucleotide sequence.…”

Section: Sequence Duplicationsmentioning

confidence: 99%

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.

et al. 1996

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“…Analysis of the URA3 and MAT segregation in the 2 TRP+:2 trptetrads showed whether the plasmid sister chromatids had separated from each other during the first or the second meiotic division. In a correctly segregating CEN plasmid (co-segregation of plasmid sister chromatids to one pole in meiosis I) the half frequency of TRPI-URA3 and TRPI-MATtetratypes must correspond to the distance of URA3 and MAT from their centromeres, which has been calculated to be 8.3 and 25.3 centimorgans, respectively (Mortimer and Schild, 1980). The frequency of URA3-MATtetratypes represents an internal control for the segregation of these genes.…”

Section: Meiotic Segregation Analysismentioning

confidence: 99%

Role of conserved sequence elements in yeast centromere DNA.

Panzeri¹,

Landonio²,

Stotz³

et al. 1985

The EMBO Journal

104

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ther outside of the 120-bp region. One mutant which just lacks the left conserved DNA element PuTCACPuTG can still assemble into a partially functional mitotic centromere and it assembles into a well functioning meiotic centromere. The sequences between the two conserved terminal DNA elements can be increased in length (+50%) or in GC content (from 6% to 12%) without measurable changes in mitotic and meiotic segregations of plasmids carrying such CEN mutations. The naturally occurring length and GC content of this centromere DNA sequence element is, therefore, not essential for centromere function. We discuss the possibility that it partly acts as a hinge region between two domains. Finally, we tested integrations of CEN DNA into the genome and found a toleration of wild-type CEN6 DNA when present 3' of the LYS2 gene.

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“…The resulting cdc9' ura3' integrant was then crossed with a cdc36 ura3 strain and tetrads dissected after sporulation. In this way it was possible to examine the linkage between the integrated ura3+ and cdc36+, a locus which is very tightly linked to cdc9 [26]. The tetrad analysis showed that the integrated uru3' was now tightly linked to cdc36+ on the left arm of chromosome IV (22 parental ditypes ; 0 non-parental ditypes ; 1 tetratype) and not at its usual location on chromosome V. Thus the common sequence in pR12Scligl and pRl2Sclig2 must derive from the cdc9 region of the genome, and since the two plasmids were isolated on the basis of functional complementation of cdc9 this result provides direct evidence that we have cloned the gene itself.…”

Section: Assa-v For Dna Ligase Activity In Yeast Transformantsmentioning

confidence: 99%