Genetic mapping and expression analysis of the murine DNA ligase I gene

Gariboldi, Manuela; Montecucco, Alessandra; Columbano, Amedeo; Ledda-Columbano, Giovanna M.; Savini, Elena; Manenti, Giacomo; Dragani, Tommaso A.

doi:10.1002/mc.2940140202

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…This is very consistent with the results of our FISH analysis using human PEG3 cDNA as a probe (data not shown). Several genes, such as Lig1, Ercc1 and Apoc2, are located between D7Mit56 and D7Mit20 in the mouse (Gariboldi et al 1995;Hoffer et al 1993). These genes are all located on human 19q13.3, whereas PEG3 is located in the telomeric region of 19q13.4.…”

Section: Discussionmentioning

confidence: 99%

Tumour suppressor activity of human imprinted gene PEG3 in a glioma cell line

et al. 2001

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Background: Mouse imprinted gene Peg3 encodes a large C2H2 type zinc finger protein with unique characteristics. Peg3 knockout mice were found to show an impairment in maternal behaviour of the adult female. Mouse Peg3 is located on the proximal region of chromosome 7 which is syntenic to the long arm of human chromosome 19. It has been reported that a loss of heterozygosity (LOH) of chromosome 19q occurs frequently in several glioma types.

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Section: Discussionmentioning

confidence: 99%

Tumour suppressor activity of human imprinted gene PEG3 in a glioma cell line

et al. 2001

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“…On the other hand a strong decrease of DNA ligase I gene expression is produced by confluence, serum starvation and cell differentiation (Camarda et al, 2004;Montecucco et al, 1992). In addition DNA ligase I expression is up-regulated during mouse liver-cell regeneration and in response to mitogenic stimuli (Gariboldi et al, 1995). The distribution of DNA ligase I is highly regulated during the cell cycle and the protein is recruited to the subnuclear sites of ongoing DNA replication (replication factories) during S-phase .…”

confidence: 99%

A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples

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Mr²,

Montecucco

et al. 2009

Eur J Histochem

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The extensive characterization of the replicative human DNA ligase I (LigI) undertaken in the last decade demonstrated that the level of this protein strongly correlates with the rate of cell proliferation. This may allow to expand the repertoire of clinical biomarkers for the analysis of cell proliferation. We have produced a new monoclonal antibody (5H5) against LigI and exploited it as cell proliferation marker in Western blotting and immunofluorescence as well as in immunohistochemistry on paraffin tissue sections. The Western blot analysis showed that the LigI level detected by 5H5 antibody is high in all proliferating cells. On the contrary the protein is down regulated in resting human fibroblast and peripheral blood lymphocytes. Immunofluorescence analysis on cultured HeLa cells showed that 5H5 antibody labels all proliferating cells and displays the same staining pattern of BrdU in S-phase nuclei. Finally the analysis of serial sections of inflamed tonsils and NHL lymph nodes (either frozen or paraffin embedded) demonstrated that 5H5 marks the same population of cells as the Ki-67 antibody. Our results demonstrate that 5H5 antibody is a valuable tool for labeling proliferating cells that can be conveniently used in Western blotting, immunocytochemistry and immunohistochemistry.

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“…The marker locus Ligl was identified by segregation analysis of the three M. spretus polymorphic bands, detected by the hybridization of HSB Taql-digested genomic DNAs with the Ligl cDNA [23]. A genetic linkage map was constructed by multipoint analysis of the data, with the MAPMARKER/EXP program [24,25].…”

Section: Genetic Mapping Of Zfp60mentioning

confidence: 99%

Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation

et al. 1996

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t. IntroductionThe formation of mature muscle fibers requires the coordin-,tted expression of many different genes coding for the myo~ube-specific proteins. These functions are governed by a set .)f regulatory genes [1]. Myogenic cell lines and myoblast 0rimary cultures can mimic many of the steps characteristic )f myogenic differentiation. The possibility to follow and to • ~tudy the in vitro transition of myoblast cells to myotube !~rovides a useful system to study gene regulation during myo-!genesis. In the last few years, several muscle regulatory genes !lave been identified and characterized such as many members • )f the MyoD gene family. This family of regulatory genes ~MyoD, myogenin, Myf5 and MRF4) share the same evoluionarily conserved domains, a basic region involved in DNA ~inding to the specific target sequence (E box), and a helix oop helix (HLH) implicated in homodimerization and/or leterodimerization with other regulatory protein [2]. The badc idea of this work is the isolation and characterization of )ther regulatory genes, involved in myogenesis, coding for ~roteins with the 'zinc finger' motif as a DNA binding donain. A zinc finger motif was first identified in the Xenopus aevis RNA polymerase III transcription factor TFIIIA [3,4]. qince then, several zinc finger genes involved in gene regulaion have been identified in a variety of animals and plants 5,6]. ~Corresponding author. Fax: (39) (6) 446 2891. E-mail: claudio@dbu.uniroma 1 .itIn a previous work we reported on the characterization of three zinc finger genes, isolated from an adult mouse skeletal muscle cDNA library [7]. In this work we describe one of those genes, termed Mfg3 and presently renamed Zfp60 according to the international convention. We determine the complete cDNA coding sequence and the predicted amino acid sequence of the ZFP60 protein. This protein is characterized by a cluster of 19 zinc finger motifs at the C-terminus and by the two Kruppel associated boxes (KRAB) A and B at the N-terminus. These modules have been shown to define a subfamily of multifinger proteins [8,9] and to be associated with transcriptional repression activity [10 13]. The expression patterns of Zfp60, MyoD and myosin heavy chain (MHC) mRNAs have been followed and compared during myoblast C2 cell line differentiation. We also show that the bacterial expressed Zfp60 protein is able to bind DNA only in presence of zinc ions. Using restriction length polymorphism (RFLP) we assigned the Zfp60 locus to the murine chromosome 7, at about 10 cM from the centromere, where a cluster of Zfp loci maps. This region shows homology with human chromosome 19q13.

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Genetic mapping and expression analysis of the murine DNA ligase I gene

Cited by 8 publications

References 22 publications

Tumour suppressor activity of human imprinted gene PEG3 in a glioma cell line

Tumour suppressor activity of human imprinted gene PEG3 in a glioma cell line

A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples

Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation

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