Genetic mapping of a mutant gene producing three pistils per floret in common wheat

Peng, Zhengsong; Martinek, Petr; Kosuge, K.; Kuboyama, Tetsuji; Watanabe, Naoharu

doi:10.1007/bf03195606

Cited by 25 publications

(18 citation statements)

References 16 publications

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“…Our result suggest a single gene, presumably, Mg, located on chromosome 2DL. The Mg gene locus may be same to the Pis1 locus reported by Peng et al (2008). However, since we cannot obtain the Trigrain line used by Peng et al (2008), it is impossible to perform the allelic test between these two trigrain lines.…”

Section: Molecular Markers For the Mg Genementioning

confidence: 88%

“…The Mg gene locus may be same to the Pis1 locus reported by Peng et al (2008). However, since we cannot obtain the Trigrain line used by Peng et al (2008), it is impossible to perform the allelic test between these two trigrain lines. The six SSR markers were physically mapped to the distal part of 2DL, with Xgwm539 and Xcfd233 the most proximally located.…”

Section: Molecular Markers For the Mg Genementioning

confidence: 88%

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Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Wang¹,

Xu²,

Ji³

et al. 2009

Can. J. Plant Sci.

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Trigrain wheat normally produces up to three gynoecia in a single floret and forms three close-set grains. The gene conferring the multi grain phenotype was earlier designated Mg, the multiple gynoecia gene. Different genetic patterns controlling this trait have been reported. In the present work we studied the inheritance of the three grains trait and identified simple sequence repeats (SSR) markers linked to the Mg gene. Segregation analysis in the cross IGDB-TW (trigrain wheat)/Chinese Spring confirmed that a single dominant gene controlled the three grains trait. An allelism test showed that the same gene controlled the trigrain trait in line Trigrain-Yin 1. A total of 339 microsatellite markers were tested for polymorphism by bulked segregation analysis (BSA) in an F2 population. Six microsatellite markers, Xcfd233, Xgdm6, Xgdm87, Xgwm311, Xgwm349 and Xgwm539, on chromosome 2DL, were linked to Mg. Using the CS 2D deletion lines, Mg gene was localized to the distal region of chromosome 2DL. The microsatellite markers identified in this study have the potential for further mapping and map-based cloning of the gene.Key words: Simple sequence repeats, physical mapping, trigrain wheat

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Section: Molecular Markers For the Mg Genementioning

confidence: 88%

Section: Molecular Markers For the Mg Genementioning

confidence: 88%

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Wang¹,

Xu²,

Ji³

et al. 2009

Can. J. Plant Sci.

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“…1a, b). Genetic analysis indicated that the three pistils trait is controlled by a single dominant gene Pis1 (Peng 2003;Peng et al 2004) located on chromosome 2DL (Peng et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat

et al. 2010

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To understand the molecular mechanism of the three pistils mutation in wheat, two forward subtractive cDNA libraries from two pairs of near-isogenic wheat lines, Chuanmai 28 three pistils (CMTP) and Chinese Spring three pistils (CSTP), were constructed using SSH. A total of 68 clones in CMTP lines and 197 clones in CSTP lines were identified as potentially over-expressed clones. Thirtytwo out of 68 clones in CMTP lines belonged to unknown proteins; while, the remaining 30 clones shared homology to diverse classes of genes involved in protein modulation and protein synthesis, signal transduction, and ion transporters. Approximately 67% of genes in CSTP lines were either unclassified or had no matches ("no hits") in the database and about 33% of identified genes encoded polypeptides with known functions. Sequence comparisons of cDNA clones between the two forward cDNA libraries revealed that four genes, encoding thioredoxin H, ubiquitin protein ligases, MCM2, and ubiquinol-cytochrome C reductase complex 14 kDa proteins, were over-expressed in both libraries. These genes would likely play an important role in determining the three pistils trait in the mutant wheat line.

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“…Genetic analysis indicated that the three-pistil trait is controlled by a single dominant gene (Peng, 2003;Peng et al, 2004). Peng et al (2008) located the gene for the TP mutation (Pis1) on chromosome 2DL by using polymorphic microsatellite markers.…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed genes in three-pistil mutation in wheat using annealing control primer system

Yang

Peng

Wei

et al. 2011

Gene

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Genetic mapping of a mutant gene producing three pistils per floret in common wheat

Cited by 25 publications

References 16 publications

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat

Identification of differentially expressed genes in three-pistil mutation in wheat using annealing control primer system

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