Genetic mapping of a mutation conferring sensitivity to bacteriophage Mu in Salmonella typhimurium LT2

Abstract

Two strains of Salmonella typhimurum LT2, SA1475 and MA411, were fortuitously found to be sensitive to bacteriophage Mu. The Mu-sensitivity allele of SA1475 was called musAl and shown to be linked to the histidine operon both in conjugation and transduction experiments. The Mu8 allele of MA411 was unlinked to the his region and was tentatively designated musB2. Strains carrying large deletions of the his operon were also tested for Mu sensitivity; those in which the his-rfb region is deleted were also sensitive to Mu. Transduction data led to the order zee-2 hisOGDCBAHFIE gnd musA. An Hfr injecting the his operon early (HfrK9) and carrying hisG9424::TnlO A4 All and musAl was isolated; this Hfr made it possible to introduce the Mu' character into most derivatives of S. typhimurium LT2. Since strain SA1475 is resistant to bacteriophage P1, it could be used to select a new Pl-Mu hybrid which has the host range of Mu and the transduction properties of P1.

Transduction of Escherichia coli trp Genes in Salmonella typhimurium and Effect of N-Methyl-N'-Nitro-N-Nitrosoguanidine on Transduction with Heterogenotic DNA

“…None of the MNNG-induced transductants exhibited increased transduction by the heterogenotic DNA. (Mergeay et al, 1981). This antimutator phenotype was previously reported in E. coli by Geiger & Speyer (1977).…”

“…It is possible that, in order to survive MNNG treatment and give rise to a recombinant, cells must mutate as they do. Perhaps these mutations (like those exhibiting the 'antimutator' phenotype: Mergeay et al, 1981) or others found during the experiments shown in Fig. 3 are more frequent in MNNG survivors.…”

Linkage map of Salmonella typhimurium, Edition VI.

“…Another has been to select mutants of Salmonella spp. that are sensitive to Mu infection (117). A fourth method has been to use Mu dl with a helper Mu phage which has tail fiber genes derived from Pl.…”

Microarray analysis of Mu transposition in Salmonella enterica, serovar Typhimurium: transposon exclusion by high‐density DNA binding proteins

“…Bacterial strains were derivatives of S. Typhimurium strain LT2. LT2 is resistant to Mu infection, but a single mutation, musA, confers sensitivity (Faelen et al, 1981). Strain NH742 is an LT2 musA MupAp1 lysogen.…”