Genetic Mapping of a Soybean Glabrous Gene Using Specific-Locus Amplified Fragment Sequencing Method

Abstract

Trichome is a natural trait related with plant defense and seed yield, however, the genetic control of this trait has not been extensively studied in soybean. In this study, a cross was made between a soybean landrace “Aijiaomaodou” with normal pubescence and a glabrous soybean line “39002”. The segregation ratio of the F2 population was fit to the expected value and the glabrous phenotype was controlled by a single dominant gene. Association analysis was conducted to identify candidate genes for the glabrous trait using a high-throughput sequencing technology, specific-locus amplified fragment sequencing. A total of 6,961 polymorphic specific-locus amplified fragments were analyzed and a region of 2.76 Mb at nucleotides 47,315,610-50,072,564 on chromosome 9 containing 551 candidate genes was identified. Four specific genes (Glyma09G280500, Glyma09G282600, Glyma09G259400 and Glyma09G283400) in the region appeared to be most important candidate genes that are highly related to the glabrous trait. Further studies of these genes may lead to isolation and cloning of the glabrous gene and facilitate the selection procedures in soybean breeding.

Genome-Wide Variation Analysis of Four Vegetable Soybean Cultivars Based on Re-Sequencing

“…The frequent usage of limited elite lines may lead to a relatively narrowed genetic background of vegetable soybean [ 20 , 21 ]. The development of related markers and the mining of specific genes can provide marker resources for molecular-marker-assisted breeding and further reveal the genome composition of the two soybean types [ 27 , 28 ]. This will have an important guiding significance for the classification of vegetable soybean breeding, and can effectively improve the directivity of vegetable soybean breeding and promote the development of the vegetable soybean industry.…”

Global Status of Vegetable Soybean

“…Gene mapping using genome-wide SNPs markers generated by polymorphic specific-locus amplified fragments identified the 2.76-Mb genome region (47,315,610–50,072,564) on chromosome 9 containing 551 candidate genes associated with the glabrous trait. Four genes including Glyma09G280500 , Glyma09G282600 , Glyma09G259400 , and Glyma09G283400 in the region appeared to be the most important candidate genes that are highly related to the glabrous trait [ 116 ].…”

Single dominant gene controls glabrous trait in vegetable soybean accession ‘AGS-406’

“…Recently, Yu et al (2020) also worked out genetic of hairy trait in a cross between soybean landrace 'Aijiaomaodou' with normal pubescence and a glabrous soybean line '39002' and reported that glabrous genotypes was controlled by single dominant gene. They also extend their study to identify genomic location of glabrous (P1) gene that was mapped to the chromosome 9 with an interval of 2.76 Mb.…”

“…Furthermore, Bernard and Singh (1969) reported considerable variation among varieties of soybeans with respect to size, shape, durability and distribution of plant hairs including P1 (glabrous), pc (curly pubescence), Pd (dense pubescence), Ps (sparse pubescence) and p2 (puberulent). Research has shown that each of the five types differed from the normal by a single gene pair and that the five loci separated independently (Yu et al 2020). Developing dark-green unblemished green pods preferably with no (glabrous pods) or sparse grey pubescence is one of the major breeding objectives in vegetable soybean.…”

“…The glabrous or grey pubescence is preferred for the vegetable soybean cultivars since the appearance of the cooked pods is clean compare to brown or dark pubescence pods. The trait has its own Vegetable Science (2021) 48 (2) : 172-177 significance as it affects diverse agronomic characters such as seed weight and yield, insect resistance, transpiration and leaf temperature regulation of plants (Hunt et al 2011;Yu et al 2020). Their role in insect resistance is much more elaborate, as some insectresistant soybean varieties had smooth leaf surface and thin trichomes while some insect-sensitive soybean varieties had rough leaf surface and dense trichomes (Komatsu et al 2007;Yang and Ye 2013;Hunt et al 2011).…”