2007
DOI: 10.1007/s11032-007-9123-9
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Genetic mapping of Echinacea purpurea via individual pollen DNA fingerprinting

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Cited by 29 publications
(32 citation statements)
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“…One work-around is to isolate and sequence individual pollen grains from these mixtures (Matsuki et al 2007;Aziz and Sauve 2008). This technique can be useful where finescale knowledge is required, but it is not practical for large-scale application and underlies the same sorting restrictions as morphology.…”
Section: Background and Potential Of Pollen Dna Barcodingmentioning
confidence: 99%
“…One work-around is to isolate and sequence individual pollen grains from these mixtures (Matsuki et al 2007;Aziz and Sauve 2008). This technique can be useful where finescale knowledge is required, but it is not practical for large-scale application and underlies the same sorting restrictions as morphology.…”
Section: Background and Potential Of Pollen Dna Barcodingmentioning
confidence: 99%
“…We had only six markers available to test (only four of which gave reliable amplification in this experiment) because of the high homozygosity in 415-1 and a consequent paucity of heterozygous SSR loci. However, more SSR markers will be available for pollen genotyping of later progenies, as successful amplification of a maximum of 104 AFLP markers was reported by Aziz and Sauve (2008).…”
Section: Dna Amplification From a Single Pollen Grainmentioning
confidence: 99%
“…However, conventional methods of identifying phytochemical traits generally require laborious field and laboratory works and are time‐consuming. Several molecular markers have been used in marker‐assisted selection programs 14, 15. These molecular techniques bypass the reliance on diagnostic morphological and phytochemical traits that take time to collect in a mass selection system.…”
Section: Resultsmentioning
confidence: 99%
“…By means of appropriate statistical methods, several DNA markers such as amplified fragment length polymorphism (AFLP), inter‐simple sequence repeat (ISSR) and randomly amplified polymorphic DNAs (RAPD) are used to quantify genetic variation among various plant species 10, 11. The high discriminating power of these techniques has also made DNA marker‐assisted selection and breeding more feasible 12–15…”
Section: Introductionmentioning
confidence: 99%