Genetic mapping of shnE.3.2 in Drosophila melanogaster

Bieser, Kayla L; Sanford, Jamie Siders; Saville, Ken; Arreola, Katherine F.; Ayres, Zachary T.; Basulto, David; Benito, Serena; Breen, Christopher J.; Brix, Julian A.; Brown, Nicole; Burton, Krissa K.; Chadwick, Taree M.; Chen, Matthew; Chu, Katherine; Corbett, Beverly L.; Dill, Zerrick; Faughender, Meghan A.; Hickey, Ashlynn D.; Julia, Joshua S.; Kelty, Shannon S.; Jobs, Briggette B.K.; Krason, Bryce A.; Lam, Brian; McCullough, Colin L.; McEwen, Bryanna R.; McKenzie, Julian L.; McQuinn, Kayla R.; Moritz, Chloe M.; Myers, Kristina E.; Naugle, Elizabeth M.; Nutter, Ashley M.; O'Conke, Danielle Q.; O'Grondik, Megan T.; Patel, Kriya B.; Rudowski, Sydney M.; Sberna, Emma N.; Stall, Gunner M.; Steiner, T; Tanriverdi, Eda; Patarroyo, Natalie Torres; Traster, Virginia L.; Tsai, Leo P.; Valenti, Andrew J.; Villegas, Mariela M.; Voors, Samantha M.; Watson, Kierra K.; Wright, Megan E.; Kagey, Jacob D.

doi:10.17912/micropub.biology.000118

Cited by 5 publications

(9 citation statements)

References 10 publications

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“…Drosophila mutant stocks contain previously generated EMS-induced mutations on the right arm of chromosome 2 (2R) (25). These mutations were previously identified based on homozygous recessive lethality and a growth-associated phenotype in the Drosophila eye when cell death is also blocked, but the genomic locus of the mutations is unknown (26,27,(29)(30)(31)(32)(33)(34)(35). The identified mutants serve as the basis for phenotypic eye characterization, complementation mapping, and molecular analysis modules of the Fly-CURE (Figure 1A,B).…”

Section: Resultsmentioning

confidence: 99%

“…Typically, students choose genes that have been previously annotated as being involved in cellular growth control, apoptosis, the cell cycle, or similar processes. In some cases, the EMS mutation fails to complement a mutant allele of a specific gene by the second round of crosses (29,32,34,35), allowing students to focus their hypothesis generation and subsequent molecular analyses on a single gene. Students then isolate genomic DNA from the mutant and control fly stocks, design PCR primers, and amplify a small (500-1000 nucleotide) region of their chosen gene.…”

Section: Resultsmentioning

confidence: 99%

“…Approximately 20% of institutions where the Fly-CURE has been taught over the last three years are also classified as Minority Serving Institutions (MSIs) (Figure 2B). Regular virtual meetings between participating faculty serve to foster collaboration between classes characterizing the same Drosophila mutation and have also culminated in eight collaborative micropublications consisting entirely of student-generated data (27,(29)(30)(31)(32)(33)(34)(35).…”

Section: The Fly-cure Provides Research Experiences At a Range Of Ins...mentioning

confidence: 99%

“…The Fly-CURE curriculum includes, but is not limited to classical Mendelian genetics, molecular genetics, and bioinformatics. Over the last ten years, students participating in the Fly-CURE have characterized over twenty novel Drosophila mutations, which have been published in eleven publications and included 581 student co-authors (26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36). Currently, the Fly-CURE is being taught at over twenty institutions across the United States.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fly-CURE, a Multi-institutional CURE usingDrosophila, Increases Students’ Confidence, Sense of Belonging, and Persistence in Research

Merkle

Devergne

Kelly

et al. 2023

Preprint

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The Fly-CURE is a genetics-focused multi-institutional Course-Based Undergraduate Research Experience (CURE) that provides undergraduate students with hands-on research experiences within a course. Through the Fly-CURE, undergraduate students at diverse types of higher education institutions across the United States map and characterize novel mutants isolated from a genetic screen inDrosophila melanogaster. To evaluate the impact of the Fly-CURE experience on students, we developed and validated assessment tools to identify students' perceived research self-efficacy, sense of belonging in science, and intent to pursue additional research opportunities. Our data show gains in these metrics after completion of the Fly-CURE across all student subgroups analyzed, including comparisons of gender, academic status, racial and ethnic groups, and parents' educational background. Importantly, our data also show differential gains in the areas of self-efficacy and interest in seeking additional research opportunities between Fly-CURE students with and without prior research experience, illustrating the positive impact of research exposure (dosage) on student outcomes. Altogether, our data indicate that the Fly-CURE experience has a significant impact on students' efficacy with research methods, sense of belonging to the scientific community, and interest in pursuing additional research experiences.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: The Fly-cure Provides Research Experiences At a Range Of Ins...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fly-CURE, a Multi-institutional CURE usingDrosophila, Increases Students’ Confidence, Sense of Belonging, and Persistence in Research

Merkle

Devergne

Kelly

et al. 2023

Preprint

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“…From this screen, we have previously reported on several conditional regulators of cell growth and cell division including CPA and Shn , demonstrating the occurrence of these conditional growth mutants [ 10 , 11 ]. Here, we describe the overgrowth caused by two of these mutants identified in the screen that are both part of the Hedgehog signalling pathway, Patched (Ptc) and Costal-2 (Cos2) .…”

Section: Introductionmentioning

confidence: 99%

Patched and Costal-2 mutations lead to differences in tissue overgrowth autonomy

Moore

Adamini

Coopes

et al. 2022

Fly

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Genetic screens are used in Drosophila melanogaster to identify genes key in the regulation of organismal development and growth. These screens have defined signalling pathways necessary for tissue and organismal development, which are evolutionarily conserved across species, including Drosophila . Here, we have used an FLP/FRT mosaic system to screen for conditional regulators of cell growth and cell division in the Drosophila eye. The conditional nature of this screen utilizes a block in the apoptotic pathway to prohibit the mosaic mutant cells from dying via apoptosis. From this screen, we identified two different mutants that mapped to the Hedgehog signalling pathway. Previously, we described a novel Ptc mutation and here we add to the understanding of disrupting the Hh pathway with a novel allele of Cos2 . Both of these Hh components are negative regulators of the pathway, yet they depict mutant differences in the type of overgrowth created. Ptc mutations lead to overgrowth consisting of almost entirely wild-type tissue (non-autonomous overgrowth), while the Cos2 mutation results in tissue that is overgrown in both the mutant and wild-type clones (both autonomous and non-autonomous). These differences in tissue overgrowth are consistent in the Drosophila eye and wing. The observed difference is correlated with different deregulation patterns of pMad, the downstream effector of DPP signalling. This finding provides insight into pathway-specific differences that help to better understand intricacies of developmental processes and human diseases that result from deregulated Hedgehog signalling, such as basal cell carcinoma.

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Fly-CURE, a multi-institutional CURE using Drosophila , increases students' confidence, sense of belonging, and persistence in research

Merkle,

Devergne,

Kelly

et al. 2023

J Microbiol Biol Educ.

View full text Add to dashboard Cite

The Fly-CURE is a genetics-focused multi-institutional Course-Based Undergraduate Research Experience (CURE) that provides undergraduate students with hands-on research experiences within a course. Through the Fly-CURE, undergraduate students at diverse types of higher education institutions across the United States map and characterize novel mutants isolated from a genetic screen in Drosophila melanogaster . To date, more than 20 mutants have been studied across 20 institutions, and our scientific data have led to eleven publications with more than 500 students as authors. To evaluate the impact of the Fly-CURE experience on students, we developed and validated assessment tools to identify students’ perceived research self-efficacy, sense of belonging in science, and intent to pursue additional research opportunities. Our data, collected over three academic years and involving 14 institutions and 480 students, show gains in these metrics after completion of the Fly-CURE across all student subgroups analyzed, including comparisons of gender, academic status, racial and ethnic groups, and parents’ educational background. Importantly, our data also show differential gains in the areas of self-efficacy and interest in seeking additional research opportunities between Fly-CURE students with and without prior research experience, illustrating the positive impact of research exposure (dosage) on student outcomes. Altogether, our data indicate that the Fly-CURE experience has a significant impact on students’ efficacy with research methods, sense of belonging to the scientific research community, and interest in pursuing additional research experiences.

show abstract

Genetic mapping of shnE.3.2 in Drosophila melanogaster

Cited by 5 publications

References 10 publications

Fly-CURE, a Multi-institutional CURE usingDrosophila, Increases Students’ Confidence, Sense of Belonging, and Persistence in Research

Fly-CURE, a Multi-institutional CURE usingDrosophila, Increases Students’ Confidence, Sense of Belonging, and Persistence in Research

Patched and Costal-2 mutations lead to differences in tissue overgrowth autonomy

Fly-CURE, a multi-institutional CURE using Drosophila , increases students' confidence, sense of belonging, and persistence in research

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