Genetic Mapping of the Thymoma Susceptible Locus, Tsr1, in BUF/Mna Rats

Oyabu, Atsushi; Higo, Kyoko; Ye, Chunlin; Amo, Hiroyuki; Saito, Mitsuhiro; Yagyu, Shigeru; Morita, Hiroyuki; Maeda, Kenji; Serikawa, Tadao; Takahashi, Masahide; Matsuyama, Mutsushi

doi:10.1093/jnci/91.3.279

Cited by 6 publications

(8 citation statements)

References 22 publications

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“…Dominant trait of the Tsr1 region was also conWrmed in this study. Thus, this congenic strain of rat will be useful for elucidation of the mechanism of the development of thymoma, analyzing the abnormality of genes which locate within the region of the Tsr1, which was deWned by the previous linkage studies (Oyabu et al 1999;Higo et al 2000). It is noteworthy that many genetic aberrations, including losses of heterozygosity and ampliWcations, were found in loci of Chr.…”

Section: Discussionmentioning

confidence: 99%

“…Genotyping of Tsr1 locus was carried out by standard polymerase chain reaction (PCR) with Wve single sequence length polymorphism (SSLP) markers, D7Wox16, D7Rat53, D7Rat21, D7Rat142 and D7Rat69 (Serikawa et al 1992, Oyabu et al 1999). …”

Section: Genotyping Of Tsr1 Locusmentioning

confidence: 99%

“…Genetic mapping of Tsr1 showed that it is located at 4.8 centiMorgans distal to the molecular marker D7Rat21 on chromosome (Chr.) 7 (Oyabu et al 1999). The Tsr1 region narrowed down into the region between D7Wox16 and D7Rat21 (Higo et al 2000).…”

Section: Introductionmentioning

confidence: 98%

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Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1

Matsuyama

Kato

Higo-Moriguchi

et al. 2008

J Cancer Res Clin Oncol

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Section: Discussionmentioning

confidence: 99%

Section: Genotyping Of Tsr1 Locusmentioning

confidence: 99%

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Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1

Matsuyama

Kato

Higo-Moriguchi

et al. 2008

J Cancer Res Clin Oncol

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“…A total of 124 {ACI × (BUF × ACI)F 1 } backcross rats were produced, and thymoma development was observed for 18 months [11]. DNA was prepared by serial extractions with phenol/chloroform and ethanol precipitation, as previously reported [9].…”

Section: Animals and Dna Preparationmentioning

confidence: 99%

“…A dominant susceptibility gene, Thymoma susceptible gene of rat 1 (Tsr1), has been mapped to a region on chromosome 7 by a linkage analysis with 124 {ACI × (BUF × ACI)F 1 } backcross rats [11]. Assuming the BUF allele of Tsr1 is indispensable for thymoma development, Tsr1 can be narrowed down to an 8.3 cM interval on the chromosome, but no more markers informative between ACI and BUF are available in the 8.3 cM interval.…”

Section: Introductionmentioning

confidence: 99%

Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis.

Higo

Oyabu

et al. 2000

Exp. Anim.

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BUF/Mna (BUF) is a rat strain susceptible to spontaneous development of thymomas. We have previously shown that the thymoma susceptibility is controlled principally by a dominant susceptibility gene located on chromosome 7, thymoma susceptibility gene of rat 1 (Tsr1). To generate genetic markers tightly linked to Tsr1, we performed genetically directed representational difference analysis (GDRDA) with three combinations of the tester and driver DNAs. From 124 {ACI/NMs × (BUF × ACI/NMs) F 1 } backcross rats, 12 rats with the ACI/BUF genotype in the Tsr1 region (A/B rats) and 13 rats with the ACI/ACI genotype in the region (A/A rats) were selected, and their DNAs were pooled, respectively. Three kinds of tester DNAs, i) inbred BUF, ii) (BUF × ACI)F 1 , and iii) the pool from the A/B rats, were subtracted by the driver DNA prepared from the pool of the A/A rats. The three combinations yielded one, two, and one polymorphic marker(s), respectively. One marker, D7Ncc28, was isolated commonly by the three combinations of subtraction, and another marker, D11Ncc12 was isolated only by the second combination. Linkage analysis demonstrated that D7Ncc28 was located in the 8.3 cM region where Tsr1 has been mapped. The three combinations of subtraction were shown to be almost equally capable of isolating polymorphic markers in a specific chromosomal region.

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Fine mapping of thymus enlargement gene 1 (Ten1) in BUF/Mna rats

Murakumo

Higo

et al. 2000

Pathology International

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Two polymeric autosomal loci, Ten1 and Ten2, regulate thymus enlargement in BUF/Mna (B) rats. Previously, we mapped Ten1 on chromosome (Chr) 1 to a 20 cM region between Myl2 and D1Mgh11, and Ten2 on Chr 13. To further characterize the precise position of Ten1, 34 and 37 microsatellite markers, that have a polymorphism between the B and WYK (W) and between the B and MITE (M) strains, were used for linkage analysis of thymus enlargement in 105 (WBF1 x B) blackcross (BC) and 78 (B x BMF1) BC rats, respectively. Our data showed that the D1Rat168, D1Rat112, D1Rat323, D1Got186, D1Got187 and D1Got188 markers each gave a peak logarithm of odds (LOD) score of 10.68 for linkage to the thymus ratio in (WBF1 x B) BC rats, and that the D1Rat168, D1Rat197, D1Got184, D1Got186 and D1Got188 markers each gave a peak LOD score of 7.82 in (B x BMF1) BC rats. The two LOD score peaks are coincident in the position of the rat genetic map. All of the markers mentioned above are located in the region between Igf2 and D1Mgh11, in which synteny is conserved with human 11q15.5 and the distal end of mouse Chr 7 or with human 11q13 and the proximal end of mouse Chr 19. Genes existing in these regions are discussed as candidate genes for Ten1.

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Genetic Mapping of the Thymoma Susceptible Locus, Tsr1, in BUF/Mna Rats

Cited by 6 publications

References 22 publications

Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1

Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1

Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis.

Fine mapping of thymus enlargement gene 1 (Ten1) in BUF/Mna rats

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