Genetic mapping of tyramine oxidase and arylsulfatase genes and their regulation in intergeneric hybrids of enteric bacteria

Abstract

Ptrin ted i'm U.S.A.

Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.

“…Since the tyn gene in strain MKNM1 is inactivated by insertion of Mu phage, this observation confirms that derepression of arylsulfatase synthesis is due to the synthesis of tyramine oxidase (24). Moreover, no activity was observed in E. coli strains C600 and CTM4 even in the presence of atsz, as shown previously (26,38). The cells of recombinants containing the tynK and atsK genes in E. coli grown with sodium sulfate or cysteine as a sulfur source had a lower level of arylsulfatase, and this repression of arylsulfatase synthesis was derepressed by addition of tyramine, whereas, as in K. aerogenes MK9000, arylsulfatase was synthesized in E. coli when the recombinant cells were grown with methionine or taurine as the sole source of sulfur.…”

“…The arylsulfatase gene from K. aerogenes could not be transferred to E. coli by using P1 clrlOOKM, because the DNA homology of the ats genes of the two bacteria is low (26,38). In this report, we succeeded in transferring the atsK gene to E. coli by intergeneric mating, using RP4 or RP4: :Mu cts and Mu cts in a recipient strain of E. coli.…”

“…Previously, we extended the host range of coliphage P1 to members of the genera used here (25) and to the strains of enteric bacteria reported by Goldberg et al (15) and created intergeneric hybrid strains of enteric bacteria by transfer of the leucine and tyramine oxidase genes between K. aerogenes, E. coli, and S. typhimurium (26). However, a low transduction frequency was observed when the donor and recipient organisms were from different bacterial genera, owing to lack of DNA homology between the selected genes (26,36). Moreover, the optimal multiplicity of infection was 0.1 to 0.5 for transduction between members of the same strain, whereas it was 5 to 10 for intergeneric transduction (26).…”

Genetic control of tyramine oxidase, which is involved in derepressed synthesis of arylsulfatase in Klebsiella aerogenes

“…This laboratory (10) MK63-1 tynP4 atsA343 P1 from MKN343-1 to MKN63 MK63-2 tynP4 atsA343 P1 from MKN343-1 to MKN63 MKN301…”

“…Transduction. P1 transduction was performed as described previously (10). Scoring of Ats and Tyn phenotypes was done as described previously (8).…”