Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA

Newton, Lisa A.; Chilton, Neil B.; Beveridge, Ian; Hoste, Hervé; Nansen, P.; Gasser, Robin B.

doi:10.1016/s0001-706x(97)00105-8

Cited by 65 publications

(44 citation statements)

References 45 publications

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“…Different ITS rDNA types have been cited by different authors in some species of strongylid nematodes (Campbell et al 1995;Stevenson et al 1995;Gasser et al 1996;Newton et al 1997Newton et al , 1998. These sequence differences among ITS repeats in the rDNA array appeared to be a consequence of (intrachromosomal) mutational exchange during DNA replication (Schlo¨tterer and Tautz 1994).…”

Section: Discussionmentioning

confidence: 99%

Determination of Trichuris muris from murid hosts and T. arvicolae (Nematoda) from arvicolid rodents by amplification and sequentiation of the ITS1–5.8S-ITS2 segment of the ribosomal DNA

et al. 2002

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Trichuris muris has been isolated from murid hosts ( Apodemus sylvaticus and Mus musculus) and Trichuris arvicolae from arvicolid rodents in Barcelona, Spain. Genomic DNA was isolated and the ITS1-5.8S-ITS2 segment from the ribosomal DNA (rDNA) was amplified and sequenced using polymerase chain reaction techniques. The ITS2 of both populations isolated from Apodemus and Mus was 382 nucleotides in length and had a GC content of about 60.73%, while the ITS2 of T. arvicolae was 442 nucleotides in length and had a GC content of about 59.8%. Furthermore, the ITS1 of Trichuris from murids was 448 nucleotides in length and had a GC content of about 56.47%, while T. arvicolae was 446 nucleotides in length and had 57.62% of GC content. A total of 161 and 173 nucleotides were observed along the 5.8S gene of T. murisand T. arvicolae, respectively; This difference in nucleotides was due to the insertion of a DNA segment (transposon) in the 5.8S sequence of the latter species. Slight intraindividual and intraspecific variations were detected in the rDNA of both species. The presence of microsatellites was observed in all of the individuals assayed. Sequence analysis of the internal transcribed spacers and the 5.8S gene demonstrated no sequence differences between T. muris isolated from both of its murid hosts. Nevertheless, clear differences were detected between the ITS2, ITS1 and 5.8S gene of T. muris and T. arvicolae. This corroborates the existence of two separate Trichuris species in murid and arvicolid hosts. Furthermore, a phylogenetic analysis was carried out and endonucleases restriction maps were elaborated for both species.

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Section: Discussionmentioning

confidence: 99%

Determination of Trichuris muris from murid hosts and T. arvicolae (Nematoda) from arvicolid rodents by amplification and sequentiation of the ITS1–5.8S-ITS2 segment of the ribosomal DNA

et al. 2002

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“…The forward primer (SSU-18PF, GTGAACCTGCRGMWGGATCA) was modified from oligonucleotide NC5 (Newton et al 1998) over the sequences of large subunit ribosomal RNA (LSU rRNAs) from several nematode species used by Blaxter et al (1998) and corresponds to positions 2671..2690 of the rRNA genes of C. elegans (GenBank Acc. Nr.…”

Section: Methodsmentioning

confidence: 99%

Molecular and morphological characterization of heterorhabditid entomopathogenic nematodes from the tropical rainforest in Brazil

Dolinski

Kamitani

Machado

et al. 2008

Mem. Inst. Oswaldo Cruz

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“…Indeed, correct morphological identification has been also precluded as an accurate diagnostic tool for many other organisms in which polymerase chain reaction together with restriction fragment length polymorphism (PCR-RFLP) have been used. This methodology provides an alternative approach for molecular differentiation and has been successfully employed to identify Biomphalaria snails , to distinct cryptic species within the Trypanosoma brucei group (Agbo et al 2001) and also to separate closely related nematodes (Newton et al 1998, Wu et al 1999, Otranto et al 2001). In addition, sequencing of the second internal transcribed spacer region (ITS2), as well as PCR-RFLP has shown to be a powerful genetic tool for studying some Metastrongylidae species (Leignel et al 1997, Conole et al 1999.…”

mentioning

confidence: 99%

Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism

Caldeira

Carvalho

Mendonca

et al. 2003

Mem. Inst. Oswaldo Cruz

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Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA

Cited by 65 publications

References 45 publications

Determination of Trichuris muris from murid hosts and T. arvicolae (Nematoda) from arvicolid rodents by amplification and sequentiation of the ITS1–5.8S-ITS2 segment of the ribosomal DNA

Determination of Trichuris muris from murid hosts and T. arvicolae (Nematoda) from arvicolid rodents by amplification and sequentiation of the ITS1–5.8S-ITS2 segment of the ribosomal DNA

Molecular and morphological characterization of heterorhabditid entomopathogenic nematodes from the tropical rainforest in Brazil

Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism

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