Genetic markers in the Spitsbergen reindeer

Abstract

Blood samples from 81 Spitsbergen reindeer together with reference samples from 11 continental Norwegian reindeer have been examined by different electrophoretic techniques with regard to the following genetic marker systems: Red cells: Hb, PGM, 6‐PGD, GPT, ESD, GLO, and AcP. Serum: A1 and Tf. In all markers except Tf (transferrin) Spitsbergen reindeer types showed the same electrophoretic patterns as did the continental Norwegian reindeer, i.e. they probably were homozygous for the alleles seen in the reference animals. In the Tf system, three different phenotypes were observed in the Spitsbergen group, designated TfG2, TfG2G3, and TfG3. The distribution of phenotypes fits well with one autosomal codominant Tf locus with two common alleles TfG2 and TfG3, neither of which has been seen in continental Norwegian reindeer. It is concluded that the Tf phenotype distribution observed indicates a relatively large genetic distance between Spitsbergen and continental Norwegian reindeer.

Estimation of biodiversity and population structure of Russian reindeer (Rangifer tarandus) breeds inhabiting Northeastern Siberia (Republic of Sakha - Yakutia) using microsatellite markers

“…Extremely important data were obtained from the study of blood serum using this method. Thus, polyacrylamide gel electrophoresis was used to analyze transferrin variation in reindeer, which was described by Storset et al (1978), Baccus et al (1983), Roed (1985), Cronin (1995). Shubin (1969) found out that in Russia a transferrin locus in reindeer is represented by 13 genetic variants.…”

“…Several genetic surveys have been conducted for reindeer, using diff erent genetic methods, such as, gel electrophoresis (Storset et al, 1978;Roed, 1985;Shubin, 1969), mitochondrial DNA markers (Flagstad and Røed, 2003;Cronin et al, 2005) and nuclear microsatellites (Ball et al, 2010;McDevitt et al, 2009;Zittlau et al, 2000;Wilson et al, 1997).…”

Interglacial remains of caribou (Rangifer tarandus) and lemming (Dicrostonyx torquatus(?)) from North Greenland

“…Recent morphometric and genetic studies have indicated that there is a close taxonomic affinity between Rangifer tarandus pearyi from the Canadian Arctic Islands, R. t. eogroenlandicur from Northeast Greenland and perhaps R. t. platyrhynchus from Svalbard (Hakala et al 1985;Rded 1985;Rded & Whitten 1986;). It has also been shown that there is comparatively little genetic similarity between the arcticisland caribou, on the one hand, and the Eurasian and mainland North American caribou and reindeer on the other (Storset et al 1978;Hakala et al 1985;Rded & Whitten 1986;Roe9 et al 1986).…”

DEVELOPMENT OF MULTIPLEX MICROSATELLITE PANEL TO ASSESS THE PARENTAGE VERIFICATION IN AND DIFFERENTIATION DEGREE OF REINDEER POPULATIONS (Rangifer tarandus)

“…Research began in the 1960s, when a simple and quick methods of electrophoresis in agar, starch and acrylamide gel were introduced in population genetics widely. Later, the studies of serum transferrin polymorphism were reported [13][14][15][16]. With the development of molecular genetics, application of more precise methods became possible for the direct analysis at the DNA level with a higher capacity as compared to the previously used marker systems [17,18].…”