Genetic-metabolic coupling for targeted metabolic engineering

Cardinale, Stefano; Farfan, Felipe Gonzalo Tueros; Sommer, Morten Otto Alexander

doi:10.1101/156927

Cited by 1 publication

(1 citation statement)

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“…This indicates that the gene gfp that was inserted into the genome increased the metabolism of the cell in the synthesis of growth hormone and the cell has the potential to be a organic fertilizer agent [10]. According to Cardinale et al [11], foreign genetic insertion into the genome of an organism (bacteria) affects the metabolic system of the cell by rearranging the metabolism, increasing the synthesis of some metabolites and decreasing the synthesis of other metabolites 10 . Furthermore, the result of the analysis shows that Giberilic Acid-3 (GA3) was synthesized with the highest percentage and Benzylaminopurine with the lowest percentage.…”

Section: B Analysis Of Extracellular Metabolites: Growth Hormonementioning

confidence: 99%

Ability to Synthesize Extracellular Metabolites of Rice Endophytic Bacteria Before and After Gfp Gene Transformation

Ikhwan¹,

Fransiska²

2018

Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018)

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The purpose of this research was to investigate the ability of rice endophytic bacteria to synthesize extracellular metabolites before and after gfp (Green Fluorescent Protein) gene transformation. These bacteria were grown in liquid media M63+2mM Tryptophan for 3 days and the supernatants were extracted with absolute methanol 1:1 (v/v). The extractants were then analysed with Gas Chromatography-Mass Spectrophotometry (GC-MS). The GC-MS result showed that all isolates that were tested be it the control (without transformation) and the mutants (transformed) were able to synthesize growth hormones with different composition patterns, namely Indole 3 Butryric Acid (IBA), Indole Acetic Acid (IAA), Kinentin, Cis Zeatin, Zeatin, Benzylaminopurine, Giberellic Acid (GA3, GA1) as well as Zeatin Riboside. Furthermore, the isolates also synthesised osmoprotectants, namely Glycine, Proline, Arabinose, Thiamine, and Trehalose with different composition patterns as well.

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Section: B Analysis Of Extracellular Metabolites: Growth Hormonementioning

confidence: 99%