Genetic modification of tomato with the tobacco lycopene β-cyclase gene produces high β-carotene and lycopene fruit

Ralley, Louise; Schuch, Wolfgang; Fraser, Paul D.; Bramley, Peter M.

doi:10.1515/znc-2016-0102

Cited by 18 publications

(12 citation statements)

References 26 publications

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“…Some sites were designed to target gene SGR1 for promoting the synthesis of lycopene, whereas others were selected from genes that catalyze the cyclisation of lycopene, such as LCY-E , LCY-B1 , and LCY-B2 , as well as Blc. LCY-E was used to prevent the cyclisation from lycopene to α-carotene and LCY-B1 and LCY-B2 were used to prevent the cyclisation from lycopene to β-carotene ( Pecker et al, 1996 ; Ronen et al, 1999 , 2000 ; Moreno et al, 2013 ; Ralley et al, 2016 ), whereas Blc is a gene that has both β-cyclase and ε-cyclase activities predicted by InterPro Database 5 .…”

Section: Resultsmentioning

confidence: 99%

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

et al. 2018

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Numerous studies have been focusing on breeding tomato plants with enhanced lycopene accumulation, considering its positive effects of fruits on the visual and functional properties. In this study, we used a bidirectional strategy: promoting the biosynthesis of lycopene, while inhibiting the conversion from lycopene to β- and α-carotene. The accumulation of lycopene was promoted by knocking down some genes associated with the carotenoid metabolic pathway. Finally, five genes were selected to be edited in genome by CRISPR/Cas9 system using Agrobacterium tumefaciens-mediated transformation. Our findings indicated that CRISPR/Cas9 is a site-specific genome editing technology that allows highly efficient target mutagenesis in multiple genes of interest. Surprisingly, the lycopene content in tomato fruit subjected to genome editing was successfully increased to about 5.1-fold. The homozygous mutations were stably transmitted to subsequent generations. Taken together, our results suggest that CRISPR/Cas9 system can be used for significantly improving lycopene content in tomato fruit with advantages such as high efficiency, rare off-target mutations, and stable heredity.

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Section: Resultsmentioning

confidence: 99%

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

et al. 2018

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“…Two recent works have achieved this goal, resulting in tomato ripe fruits that were orange instead of red when ripe. In one of them, expression of a N. tabacum gene encoding LCYB driven by the Arabidopsis UBQ3 promoter resulted in enhanced levels of β-carotene in tomato fruit (up to 3-fold, 783 μg/g DW) with negligible changes of other carotenoids, including lycopene [259]. The second work investigated the activity of the fruit Clp protease during ripening [66].…”

Section: Tomato (Solanum Lycopersicon)mentioning

confidence: 99%

A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health

Rodríguez‐Concepción

Ávalos

Bonet

et al. 2018

Progress in Lipid Research

754

584

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Carotenoids are lipophilic isoprenoid compounds synthesized by all photosynthetic organisms and some non-photosynthetic prokaryotes and fungi. With some notable exceptions, animals (including humans) do not produce carotenoids de novo but take them in their diets. In photosynthetic systems carotenoids are essential for photoprotection against excess light and contribute to light harvesting, but perhaps they are best known for their properties as natural pigments in the yellow to red range. Carotenoids can be associated to fatty acids, sugars, proteins, or other compounds that can change their physical and chemical properties and influence their biological roles. Furthermore, oxidative cleavage of carotenoids produces smaller molecules such as apocarotenoids, some of which are important pigments and volatile (aroma) compounds. Enzymatic breakage of carotenoids can also produce biologically active molecules in both plants (hormones, retrograde signals) and animals (retinoids). Both carotenoids and their enzymatic cleavage products are associated with other processes positively impacting human health. Carotenoids are widely used in the industry as food ingredients, feed additives, and supplements. This review, contributed by scientists of complementary disciplines related to carotenoid research, covers recent advances and provides a perspective on future directions on the subjects of carotenoid metabolism, biotechnology, and nutritional and health benefits.

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“…Phenotypic changes in carotenoids also found in vegetative tissues, but levels of biosynthetically related isoprenoids such as tocopherols, ubiquinone, and plastoquinone. [4] Green plants also possess several different classes of bioorganic compounds, i.e., terpenoids, triterpene acids, phenolic acids, hydroquinone, flavonoids, hydrocarbons, sterols, fatty acids, tocopherols, and inorganic compounds but pigments show best anticancer [5] and anti-melanogenesis efficacy [ Table 1]. [3] Few vegetables like tomato (Solanum lycopersicum) are rich in anthocyanins and lycopene.…”

Section: Review Articlementioning

confidence: 99%

“…[1] Anthocyanins and carotenoids found in Japanese blue tomato [8] are strong anticancer agents. [4] Natural pigments such as anthocyanin, [9] hypocrellin A, [10] theacitrin A-C, [11] carotenoids, and green photopigments are considered best phytomedicine for cancer prevention. [12] Anthocyanins are phenolic pigments show cell protective effects [8] and show cytotoxicity to MCF-7 tumor cells.…”

Section: Review Articlementioning

confidence: 99%

Untitled

Upadhyay¹

2018

IJGP

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Present review article explains the dietary use of plant pigments and therapeutic effects against cancer. Important plant pigments such as anthocyanins, lycopene, carotenoids, chlorophyll, and betalains are explained for their anticancer effects. Plant pigments are secondary metabolites which obstruct cancer cell proliferation; stop growth and cell division in cancer cells. These inhibit cellular processes in cancer cells such as signaling pathways, cell cycle, induce apoptosis, and autophagy. Besides, anticancer activity these also assist in controlling high blood pressure, obesity, hyperglycemia, hypercholesterolemia, and restore cardiovascular problems. A full series of pigments is available in various plants families which might show protective effects against cancer. Plant pigments are edible, nutritionally rich and therapeutically suitable. Due to their health-promoting effects there is a growing public interest to consume green vegetables, fruits, sprouted seeds, pigmented cereals, and processed low energy antioxidative functional food. For widening their use, these could be harvested using recombinant gene technology to add to processed foods as a coloring agent. Plant pigments as natural plant products or its by-products are highly useful for the development of a large variety of functional foods, digestive ingredients, additives, as well as cosmetic products. These could be naturally added to genetically suitable modified foods by applying genomic tools. No doubt plant secondary metabolites will also fulfill needs of present-day medicine and show great promise for the future.

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Genetic modification of tomato with the tobacco lycopene β-cyclase gene produces high β-carotene and lycopene fruit

Cited by 18 publications

References 26 publications

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health

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