Genetic monitoring of populations of Matthiola Fragrans (Bunge) using RAPD and AFLP analysis

Khadeeva, N. V.; Goryunova, S. V.; Kochumova, A. A.; Yakovleva, Ye. Yu.; Melnikova, N. V.; Zholobova, O. O.; Korotkov, O. I.; Kudryavtsev, A. M.

doi:10.1134/s1062359011040078

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…High heterogeneity of poplar populations in Moscow and widespread distribution of interspecific hybrids were shown (Kostina, Nasimovich, 2014;Kostina et al, 2017). In addition to morphological features, the use of molecular markers is effective for plant diversity evaluation (Melnikova et al, 2009(Melnikova et al, -2011Khadeeva et al, 2011;Bolsheva et al, 2015). In our work, we first applied high-throughput sequencing of ITS to assess the genetic diversity of poplars in Moscow.…”

Section: Resultsmentioning

confidence: 99%

Genetics polymorphism of poplars from Moscow region based on high-throughput sequencing of ITS

Borkhert¹,

Krasnov²,

Bolsheva³

et al. 2018

Vestn. VOGiS

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1 Институт молекулярной биологии им. В.А. Энгельгардта Российской академии наук, Москва, Россия 2 Российский университет дружбы народов, Москва, Россия Тополь широко используется в озеленении Москвы благо-даря способности эффективно очищать воздух от вредных примесей и выделять большое количество кислорода. Роду Тополь (Populus) свойствен высокий уровень внутривидо-вого полиморфизма, а также наличие естественных меж-видовых гибридов. Целью на стоящей работы была оценка генетического разнообразия тополей, растущих на терри-тории города Москвы, с использованием высокопроизво-дительного секвенирования внутренних транскрибируе-мых спейсеров генов 45S рРНК (ITS-последовательностей). На платформе Illumina (MiSeq) проведено секвенирование ITS-по следовательностей 40 растений тополя и в среднем получено около 3 000 прочтений для каждого образца. Биоинформатиче ская обработка данных проведена с использованием программы CLC Genomics Workbench. Исследованная выборка тополей имела высокий уровень генетического разнообразия: число выявленных в каждом генотипе однонуклеотидных полиморфизмов (SNP) от-носительно референсных последовательностей ITS1 и ITS2 P. trichocarpa варьировало от 4 до 44. Показано, что даже деревья, посаженные на одной территории и, вероятно, в одно время, зна чительно различаются генетически. Можно предположить, что при посадке тополей в Москве исполь-зовался крайне полиморфный растительный материал. Для некоторых сайтов c SNP у одного и того же индивидуума выявлено несколько вариантов нуклеотидов, соотношение которых было различным. Мы предполагаем, что соот-ношение, близкое к 50/50, наблюдается в межвидовых гибридах и является следствием генетических различий в ITS-по следовательностях между материнским и отцовским Poplars are widely used in landscaping of Moscow due to the ability to effectively purify the air from harmful impurities and to release a large amount of oxygen. The genus Populus is characterized by a high level of intraspecies polymorphism, as well as the presence of natural interspecies hybrids. The aim of our work was to evaluate the genetic diversity of poplars, which are growing on the territory of Moscow city by high-throughput sequencing of internal transcribed spacers of 45S rRNA genes (ITS sequences). Sequencing of ITS of 40 poplar plants was performed on Illumina platform (MiSeq) and about 3 000 reads were obtained for each sample in average. Bioinformatics analysis was performed using CLC Genomics Workbench tool. The involved set of poplars had a high level of genetic diversity -the number of single nucleotide polymorphisms (SNPs) detected in each genotype relative to the reference ITS1 and ITS2 sequences of P. trichocarpa varying from 4 to 44. We showed that even trees which had been planted on the same territory and, probably, at the same time had significant genetic differences. It can be speculated that highly polymorphic plant material was used for planting poplars in Moscow. For some sites with SNPs, several variants of nucleotides were found in the same individual and the ...

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Section: Resultsmentioning

confidence: 99%

Genetics polymorphism of poplars from Moscow region based on high-throughput sequencing of ITS

Borkhert¹,

Krasnov²,

Bolsheva³

et al. 2018

Vestn. VOGiS

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“…Естественная растительность играет важнейшую экосистемную, экологическую, биологическую, а также биоресурсную роль как резервата диких сородичей культурных, пищевых и лекарственных растений [Khadeeva et al, 2011;Zohary et al, 2012;Dzyubenko, 2013;Kulikov et al, 2013;Toropova et al, 2016].…”

Section: Introductionunclassified

Численность популяции жука-оленя lucanus cervus (l.) Лесного массива в Воронежской области, рассчитанная по пищевым остаткам сорокопута-жулана lanius collurio (l.)

Голуб,

Шестопалов,

Масалыкин

2021

ПЖБ

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“…Chalk substrates are characterized by low average daily tem perature fluctua tions and low er average annual surface tem perature in com parison w ith zonal soils. The flora o f such territo ries is characterized by a high degree o f endem ism and uniqueness (Golitsyn 1956, A bram ova 1973, Khadeeva et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Impact Of Endemic Calciphilous Flora Of The Central Russian Upland On The Nitrogen Regime Of Carbonate Soils And Sub-Soils

Cherniavskih¹,

Dumacheva²,

Лисецкий³

et al. 2019

Biosci. Biotech. Res. Comm

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Unique plant communities that are com plementary to the cretaceous substrate tend to be formed on the cretaceous outcrops of southern European Russia. They are characterized by a wide spread of calciphilous species of higher plants, including endemic ones. Under these conditions, stable plant aggregations are created by the species that are able to populate substrates being toxic to most plants. The study was conducted in order to examine the nitrogen status o f soils and sub-soils under endemic calciphilous species: M atthiola fragrans Bunge, Hyssopus cretaceus Dubjan. and Andorsace koso-poljanskii Ovcz. The research tasks included a comparative analysis of the dynamic changes in the content of organic m atter carbon, total nitrogen, easily hydrolysable nitrogen, nitrate nitrogen, and nitrification capacity of soils and sub-soils in the course of vital processes of the species under study. It has been found that during the ten-year period of the experiment for M. fragrans Bunge, H. cretaceus Dubjan significantly increases the num ber of particles of <1 mm 594 ARTICLE INFORMATION:

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Genetic monitoring of populations of Matthiola Fragrans (Bunge) using RAPD and AFLP analysis

Cited by 5 publications

References 3 publications

Genetics polymorphism of poplars from Moscow region based on high-throughput sequencing of ITS

Genetics polymorphism of poplars from Moscow region based on high-throughput sequencing of ITS

Численность популяции жука-оленя lucanus cervus (l.) Лесного массива в Воронежской области, рассчитанная по пищевым остаткам сорокопута-жулана lanius collurio (l.)

Impact Of Endemic Calciphilous Flora Of The Central Russian Upland On The Nitrogen Regime Of Carbonate Soils And Sub-Soils

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