Genetic Nature of Regression of Rous sarcoma Virus-Induced Tumors in Crosses of Regional Poultry Research Laboratory Lines 6 and 72,

Abstract

Regional Poultry Research Laboratory (RPRL) inbred lines 6(1), 6(3), and 7(2) and F1, F2, and reciprocal backcross progenies of these lines were used to investigate host-gene effects upon the regression of Rous sarcoma virus (RSV)-induced tumors. Lines 6(1) and 6(3) were susceptible to subgroup A and C lymphoid leukosis (LL) viruses and line 7(2) was resistant to subgroup A but was segregating for susceptibility to subgroup C LL virus. Viruses of RSV subgroups A and C were used. Lines 6(1), 6(3), and 7(2) were homozygous for shared blood group alleles B2, C5, L1 and r. The incidence of tumor regression was higher in line 6(3) than in 7(2), and in reciprocal F1 crosses of these lines was of the same order of magnitude as in line 6(3). Progeny from F1 generation parents mated to line 6(3) had a higher frequency of regression than offspring from F1 generation parents mated to line 7(2). The frequency of regression in F2 generation offspring was intermediate between the two backcrosses. The data suggest that either a locus (or loci) other than L and B has a role in Rous tumor regression in this species or the immune response region of the B blood group-major histocompatibility complex differs in the two lines even though the serological and/or graft vs. host regions have not been shown to differ.

Chicken Major Histocompatibility Complex and Disease

“…The importance of non-MHC loci in this anti-tumour response may derive from studies on chicken lines 6 and 7 from East Lansing [22,59]. These two strains are identical at the B-F and B-G loci, yet display differences in their ability to regress RSV-induced tumours.…”

“…These two strains are identical at the B-F and B-G loci, yet display differences in their ability to regress RSV-induced tumours. This may be explained by a role for non-MHC loci [31]), but a lack of identity at the B-L locus has not been excluded [59].…”

Genetics of the response to Rous sarcoma virus‐induced tumours in chickens*

“…These were R1 (BE4-G23), R2 and R3 (Bn-Ga) and R5 (BP-l-G19). Marks et al (1979) investigated Rous sarcoma regression in RPRL lines 63, 7, and their reciprocal backcross progeny. Using these four recombinants, we have shown (Collins & Briles, 1980;Auclair et al, 1981; Aeed, unpublished data) that a gene influencing tumour regression lies within the B-F chromosomal region, or in a region closely linked to it, but not B-G.…”