Genetic Parameters and Genome-Wide Association Studies for Anti-Müllerian Hormone Levels and Antral Follicle Populations Measured After Estrus Synchronization in Nellore Cattle

Grigoletto, Laís; Santana, Miguel Henrique de Almeida; Bressan, Fabiana Fernandes; Eler, Joanir Pereira; Nogueira, Marcelo Fábio Gouveia; Kadarmideen, Haja N.; Baruselli, Pietro Sampaio; Ferraz, José Bento Sterman; Brito, Luiz F.

doi:10.3390/ani10071185

Cited by 20 publications

(15 citation statements)

References 95 publications

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“…The increase in follicular size increases the AMH level. The dynamic expression pattern marks an AMH to be a potential endocrine marker to the size of ovarian pool, to improve reproduction rates and for the selection of better oocyte donor ( 9 , 13 , 21 , 26 ). In our present study, we report for the first time the complete coding sequence of AMH in Indian riverine buffalos and goats.…”

Section: Discussionmentioning

confidence: 99%

“…Small follicles from healthy ovaries of Indian riverine buffaloes and goats were aspirated as described ( 25 , 26 ), and the pooled fluid containing granulosa cells were centrifuged at 500 × g for 5 min followed by washing twice with 1x PBS. RNA was isolated according to the manufactures protocol using TRI reagent (T9424, Sigma Aldrich, Merck, Darmstadt, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…These observations strongly suggest that AMH is a dominant regulator of early follicular growth. AMH hormone levels and antral follicle populations can be used as a marker to genetically improve fertility rates in Nelore cattle and to screen for better oocyte donors ( 26 ). The AMH gene has not been characterized in many domestic animals, specifically large as well as small ruminant species.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Anti-Müllerian Hormone (AMH) Gene in Buffaloes and Goats

et al. 2021

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The Anti-Müllerian Hormone (AMH) is a member of the transforming growth factor beta (TGF-β) superfamily, playing a significant role in cell proliferation, differentiation and apoptosis. In females, AMH is secreted throughout their reproductive life span from ovaries, whereas in males it is secreted by gonadal cells at a very early stage of testicular development. AMH is a promising marker of ovarian reserve in women and can be used to measure the female reproductive lifespan. In the present study, we cloned and sequenced the GC rich AMH gene from Indian riverine buffalo (Bubalus bubalis) and goat (Capra hircus). Obtained sequences were compared to the AMH sequences of other mammals, and corresponding amino acid sequences revealed that the caprine and bovine AMH sequences are more closely related to each other than to those of other mammals. Furthermore, we analyzed the chromosomal localization of AMH genes in mammalian species to understand potential syntenic relationship. The AMH gene is localized between the sequences for the SF3A and JSRP1 genes and maintains this precise location in relation to other nearby genes. The dN/dS ratio of AMH gene did not indicate any pressure for either positive or negative selection; thus, the physiological function of the AMH gene in the reproduction of these two ruminant species remains very vital. Similar to other mammals, the AMH gene may be an important indicator for regulating female reproductive biology function in bovine, cetacean, caprine, and camelidae.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of Anti-Müllerian Hormone (AMH) Gene in Buffaloes and Goats

et al. 2021

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“…They reported the pedigree-based heritability of AMH as 0.43±0.07 and the genomic heritability of AMH as 0.36 ± 0.03 (Nawaz et al 2018). In another study, the estimation of genomic heritability of AMH in Nellore cattle (n=944) was reported as 0.28 ± 0.07 (Grigoletto et al 2020). Gobikrushanth et al (2018) indicated a high (0.46 ± 0.31) heritability estimate for AMH in Holstein cows (n=198).…”

Section: Genomic Heritability Of Amhmentioning

confidence: 96%

Anti Mullerian Hormone: A Putative Endocrine Marker for Prediction of Superovulation Response in Cattle

Ünal

2021

Black Sea Journal of Agriculture

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Anti-Müllerian Hormone (AMH) is a dimeric glycoprotein molecule with a molecular weight of 140-kDa linked to the Transforming Growth Factor-β (TGF-β) superfamily. Research on the use of AMH in livestock has gained momentum in recent years. In particular, it is now widely used in cattle breeding, where embryo transfer technology is used to obtain more offspring from genetically superior females. One of the most important factors that increase the success of embryo transfer is the response of the selected donor to the superovulation protocol. AMH has been successfully used as a biomarker in predicting superovulation response in cattle and in estimating the numbers of oocytes collected by ovum pick up (OPU). AMH plasma concentrations are positively and highly correlated with antral follicle count (AFC) in cattle and can also be used as a marker of ovarian reserve. In addition, AMH was also positively and highly correlated with the number of corpus luteum (CL) and total embryos after superovulation in several studies. It has been also reported via Genome-Wide Association Studies (GWAS) that plasma AMH level is an inherited trait in cattle and can be improved through genomic selection. In this study, we aimed to evaluate the relationship between plasma AMH levels and superovulation response in cattle by compiling the data obtained from various studies in light of current scientific literature.

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“…Genetic variation has been identified that is associated with AFC in beef heifers on chromosomes 2, 3, and 23 (Stegemiller et al, 2021). In Nellore cattle, regions of interest included chromosomes 1 and 26, which accounted for 6.2 and 7.3% of the total additive genetic variance, respectively (Grigoletto et al, 2020). For AGD, strong genetic markers are yet to be reported in cows (Gobikrushanth et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Investigating anogenital distance and antral follicle count as novel markers of fertility within a herd of cows with positive or negative genetic merit for fertility traits

Grala

Price

Kuhn-Sherlock

et al. 2021

Journal of Dairy Science

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Using early-in-life markers of reproductive characteristics may enhance the speed and success of genetic improvement in fertility. We investigated 2 phenotypes that can be measured early in life and are moderately heritable to determine their association with traditional measures of reproductive success and genetic variation under a seasonal-calving, pasture-based system. Cows were bred to be divergent in the New Zealand Fertility Breeding Value, which estimates genetic merit for fertility. Cows consisted of 2 groups with an average positive (+5%) or negative (−5%) genetic merit for fertility traits and were expected to have large diversity in reproductive outcomes. Calves were genotyped at 41 ± 3.1 d of age (mean ± SD; n = 538), and antral follicle counts (AFC) were measured when they were postpubertal heifers before their first breeding (416 ± 15 d old; 92-d range; n = 520). The anogenital distance (AGD) was measured in 478 primiparous cows of this same population 50 to 60 d after the breeding start date when they were 881 ± 25 d old (145-d range). The AGD was shorter in animals with a positive genetic merit for fertility traits (based on parent averages). An indicator of herd reproductive success in a seasonalcalving system (recalving by 6 wk in lactation 2) was chosen for logistic regression with cross-validation, and if significant, a cut-off was calculated that categorized animals into groups. Both linear and quadratic regression was undertaken, and the model with the greatest sensitivity for detection of nonpregnant cows used. The AGD linear model was significant with a sensitivity of 64% and a specificity of 48%. This model resulted in a cut-off of 102 mm, which we used to classify cows as short (≤102 mm) or long (>102 mm) AGD animals. Primiparous cows with a short AGD were more likely to be pregnant within the first 3 and 6 wk of mating, and become pregnant as a primiparous cow, than those with a long AGD. The time from calving to conception was 20 d earlier in short AGD compared with long-AGD cows. None of the models tested for AFC were significant; therefore cows were categorized into 3 groups based on previous work in seasonal systems. However, associations between fertility phenotypes and AFC group were limited. Genomic regions of interest for AGD and AFC did not overlap, indicating phenotypes were genetically independent. Overall, AGD appears as a promising early marker of fertility in seasonal grazing systems.

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Genetic Parameters and Genome-Wide Association Studies for Anti-Müllerian Hormone Levels and Antral Follicle Populations Measured After Estrus Synchronization in Nellore Cattle

Cited by 20 publications

References 95 publications

Characterization of Anti-Müllerian Hormone (AMH) Gene in Buffaloes and Goats

Characterization of Anti-Müllerian Hormone (AMH) Gene in Buffaloes and Goats

Anti Mullerian Hormone: A Putative Endocrine Marker for Prediction of Superovulation Response in Cattle

Investigating anogenital distance and antral follicle count as novel markers of fertility within a herd of cows with positive or negative genetic merit for fertility traits

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