Genetic parameters of muscle fatty acid profile in a purebred Large White heavy pig population

Zappaterra, Martina; Catillo, G.; Belmonte, Anna; Fiego, Domenico Pietro Lo; Zambonelli, Paolo; Steri, Roberto; Buttazzoni, L.; Davoli, Roberta

doi:10.1016/j.meatsci.2020.108057

Cited by 13 publications

(17 citation statements)

References 45 publications

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“…This set of samples consisted of 12 individuals, chosen from a purebred population of 949 sib-tested ILW pigs, for their extreme and divergent contents of SM IMF (low IMF vs. high IMF group). A detailed description of the population of origin and of the 12 samples was reported in Zappaterra et al [11] and Zappaterra et al [13], respectively. The pigs entered the testing station at about 30 kg live weight and were reared in the same environmental conditions.…”

Section: Sampling and Phenotypesmentioning

confidence: 99%

“…IMF was determined in % (g/100 g of muscle tissue). SM FA composition was determined as described in Zappaterra et al [11]. The FA and FA classes were expressed as mg/g of IMF.…”

Section: Sampling and Phenotypesmentioning

confidence: 99%

“…The gene count matrix was analyzed together with the IMF contents of palmitic, palmitoleic, stearic, oleic, n-6, and n-3 FAs. These FAs were chosen for their relative abundance in IMF (palmitic, palmitoleic, stearic, and oleic are the saturated FAs (SFAs) and monounsaturated FAs (MUFAs) most represented in pork fat tissues [6,11]) and for their nutritional value (n-6 and n-3 PUFAs).…”

Section: Weighted Gene Correlation Network Analysis (Wgcna)mentioning

confidence: 99%

“…The original ILW pig population (949 pigs) described in our previous paper [11] had an average content of IMF of 2.15 ± 1.13% and a hot carcass weight of 114.50 ± 8.64 kg. In that population, the SM FAs most represented were palmitic acid (with an average content of 23.51 ± 1.14%), palmitoleic acid (2.93 ± 0.49%), stearic acid (11.88 ± 1.19%), oleic acid (40.72 ± 2.89%), n-6 PUFAs (13.96 ± 3.12%) and n-3 PUFAs (0.60 ± 0.12%).…”

Section: Descriptive Statisticsmentioning

confidence: 99%

“…So far, efforts have been made to improve both technological and nutritional quality traits, and an extensive body of literature focuses on the multiple variation factors affecting meat quality and FA composition of the final meat products [5,6,9,10]. Studies carried out in Italian Large White (ILW) pigs found that the FA composition of IMF can be improved through selection [11] and is associated with genetic markers [12]. In fact, muscle FA composition showed low to medium heritability estimates, ranging from 0.157 to 0.237 in ILW pigs.…”

Section: Introductionmentioning

confidence: 99%

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Dissecting the Gene Expression Networks Associated with Variations in the Major Components of the Fatty Acid Semimembranosus Muscle Profile in Large White Heavy Pigs

Zappaterra

Gioiosa²,

Chillemi

et al. 2021

Animals

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To date, high-throughput technology such as RNA-sequencing has been successfully applied in livestock sciences to investigate molecular networks involved in complex traits, such as meat quality. Pork quality depends on several organoleptic, technological, and nutritional characteristics, and it is also influenced by the fatty acid (FA) composition of intramuscular fat (IMF). To explore the molecular networks associated with different IMF FA compositions, the Semimembranosus muscle (SM) from two groups of Italian Large White (ILW) heavy pigs divergent for SM IMF content was investigated using transcriptome analysis. After alignment and normalization, the obtained gene counts were used to perform the Weighted Correlation Network Analysis (WGCNA package in R environment). Palmitic and palmitoleic contents showed association with the same gene modules, comprising genes significantly enriched in autophagy, mitochondrial fusion, and mitochondrial activity. Among the key genes related to these FAs, we found TEAD4, a gene regulating mitochondrial activity that seems to be a promising candidate for further studies. On the other hand, the genes comprised in the modules associated with the IMF contents of oleic, n-6, and n-3 polyunsaturated FAs (PUFAs) were significantly enriched in Mitogen-Activated Protein Kinase (MAPK) signaling, in agreement with previous studies suggesting that several MAPK players may have a primary role in regulating lipid deposition. These results give an insight into the molecular cascade associated with different IMF FA composition in ILW heavy pigs. Further studies are needed to validate the results and confirm whether some of the identified key genes may be effective candidates for pork quality.

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Section: Sampling and Phenotypesmentioning

confidence: 99%

“…IMF was determined in % (g/100 g of muscle tissue). SM FA composition was determined as described in Zappaterra et al [11]. The FA and FA classes were expressed as mg/g of IMF.…”

Section: Sampling and Phenotypesmentioning

confidence: 99%

Section: Weighted Gene Correlation Network Analysis (Wgcna)mentioning

confidence: 99%

Section: Descriptive Statisticsmentioning

confidence: 99%