Genetic polymorphism detection in bone morphogenetic protein 15 (BMP15) gene related to fecundity in two Egyptian sheep breeds

El, Fiky Zaki A.; Hassan, Gamal M.; Nassar, Mohamed I.

doi:10.2298/bah1701037e

Cited by 3 publications

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References 23 publications

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“…The main aim of sheep production is for one or more of the following purposes: wool, milk, and meat (El Fiky et al, 2017). However, all the breeds of sheep in Ghana are kept for meat and therefore growth and carcass composition are of commercial importance for mutton production.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of selected candidate genes in Ghanaian sheep breeds

Bekoe,

Kayang,

Adenyo

2024

GJAS

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Polymorphisms of candidate genes BMP15 and GDF9, GH and CAST that influence fecundity, growth, and carcass traits, respectively, were studied in two Ghanaian sheep breeds to determine whether these breeds were polymorphic or monomorphic at the above gene loci. DNA was extracted from blood samples collected from 63 Sahel and 49 Djallonké sheep and analyzed by polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) technique. Restriction enzyme digestion of the BMP15-FecXB locus revealed two genotypes (AA and AB) in both populations with genotype AA recording the highest frequency in both populations. At the BMP15- FecXG locus, genotype Gg had the highest frequency (0.548) in the Djallonké population whereas GG frequency (0.719) was the highest in the Sahel population. Analysis of GDF9-FecGH with DdeI endonuclease also revealed two genotypes, AA and AB in both populations with the genotypic frequency of AA being the highest in both populations (Sahel= 0.933 and Djallonké= 0.98). At the GH gene locus, restriction digestion with HaeIII showed three genotypes (AA, AB, BB), with genotype AB recording the highest frequency in both populations. In addition, PCR-RFLP analysis at the CAST-MspI gene locus showed three genotypes (MM= 0.048, MN= 0.919, NN= 0.032) in the Sahel population whereas only two genotypes (MM= 0.367, MN= 0.633) were observed in the Djallonké population. Results from this study showed that the selected candidate genes were polymorphic in the two studied sheep breeds of Ghana. These results will form the basis for candidate gene association studies in the two sheep breeds.

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Section: Introductionmentioning

confidence: 99%

Polymorphism of selected candidate genes in Ghanaian sheep breeds

Bekoe,

Kayang,

Adenyo

2024

GJAS

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Genetic polymorphism of growth differentiation factor 9 (GDF9) gene related to fecundity in two Egyptian sheep breeds

Fiky

Hassan

Nassar

2017

J Assist Reprod Genet

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G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

et al. 2021

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Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types. Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types. Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p≤0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor. Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression.

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Genetic polymorphism detection in bone morphogenetic protein 15 (BMP15) gene related to fecundity in two Egyptian sheep breeds

Cited by 3 publications

References 23 publications

Polymorphism of selected candidate genes in Ghanaian sheep breeds

Polymorphism of selected candidate genes in Ghanaian sheep breeds

Genetic polymorphism of growth differentiation factor 9 (GDF9) gene related to fecundity in two Egyptian sheep breeds

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

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