Genetic polymorphism for IFNγ +874T/A in patients with acute toxoplasmosis

Abstract

Introduction: A single nucleotide polymorphism (SNP) in the gene encoding gamma interferon influences its production and is associated with severity of infectious diseases. This study aimed to evaluate the association of IFNγ+874T/A SNP with duration of disease, morbidity, and development of retinochoroiditis in acute toxoplasmosis. Methods: A case-control study was conducted among 30 patients and 90 controls. Results: Although statistical associations were not confirmed, A-allele was more common among retinochoroiditis cases and prolonged illness, while T-allele was more frequent in severe disease. Conclusions: Despite few cases, the results could indicate a relation between IFNγ+874T/A single nucleotide polymorphism and clinical manifestations of toxoplasmosis.

Single nucleotide polymorphisms in the interferon gamma gene are associated with distinct types of retinochoroidal scar lesions presumably caused by Toxoplasma gondii infection

“…Neves et al (2009) identified the A allele in most of the individuals with toxoplasmosis-related retinochoroiditis (albeit with no statistical significance), also suggesting an association between the A allele in homozygosis and ocular toxoplasmosis. In addition, Neves et al (2012) identified an association between the AA genotype in the SNP +874T>A ( rs2430561 ) and a longer duration of symptoms in the acute stage of disease; however, the association failed to reach statistical significance, which the authors attributed to the relatively small sample size.…”

“…Indeed, the data reported by Neves et al (2012) indicate that individuals with low-IFN-γ-producer genotypes tend to exhibit a longer duration of symptoms in the acute phase of toxoplasmosis, suggesting an inability to inhibit parasite replication. If this hypothesis is confirmed, i.e., if scar lesions exhibited by high-IFN-γ producers with genotype AA are of congenital origin, then the role we have attributed to IFN-γ in the aggravation of retinochoroidal lesions will have to be reevaluated in the context of ocular toxoplasmosis.…”

“…This cytokine seems to be important in both cases, namely, in the exacerbated and in the regulated context of the in vitro cellular immune response, suggesting that the cellular immune responses against T. gondii in the eye should be suitably tailored ( Bahia-Oliveira et al 2009 , 2012 , Garweg & Candolfi 2009 ). Single nucleotide polymorphisms (SNPs), such as +874T>A ( rs2430561 ) in the IFN-γ gene ( IFNG ) that determine high (TT), low (AA) and intermediate (TA) responder phenotypes have been associated with the susceptibility to and the outcomes of infectious and chronic inflammatory diseases ( Popadic et al 2012 ), including retinochoroiditis toxoplasmic susceptibility ( de Albuquerque et al 2009 ) and the persistence of symptoms during the acute phase of infection ( Neves et al 2012 ).…”

Estudo da associação entre paracoccidioidomicose e os polimorfismos dos genes IL12B (posição 3' UTR+1188 A/C), IL12RB1 ( posição 11014 A/G no éxon 7) e IFNG ( posição + 874 T/A)

“…Quanto à distribuição dos genótipos e dos alelos no estudo do SNP IFNG +874 T/A, no presente trabalho, houve predomínio do alelo A e menor frequência do genótipo TT nos grupos de pacientes e controles, de acordo com outros estudos na população brasileira (Visentainer et al, 2008;Albuquerque et al, 2009;Matos et al, 2007;Cardoso et al, 2010;Medina et al, 2011;Bozzi et al, 2009;Neves et al, 2012;Pereira et al, 2015).…”

“…Dissertação de Flávia Mendes da Cunha Holanda na toxoplasmose (Neves et al, 2012); e na leishmaniose tegumentar americana (Matos et al, 2007).…”