Genetic polymorphism of Babesia bovis merozoite surface antigens-2 (MSA-2) isolates from bovine blood and Rhipicephalus annulatus ticks in Israel

Molad, T.; Fleiderovitz, Ludmila; Leibovich, B.; Wolkomirsky, R.; Erster, Oran; Roth, Asael; Mazuz, Monica L.; Markovics, A.; Shkap, Varda

doi:10.1016/j.vetpar.2014.07.016

Cited by 15 publications

(14 citation statements)

References 34 publications

(60 reference statements)

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“…The involvement of genetic diversity in the immune evasion of B. bovis is well documented in the literature (Berens et al, 2005;Leroith et al, 2005). Consequently, the genetic diversity among the MSA genes has been studied in several endemic countries (Altangeral et al, 2012;Berens et al, 2005;Borgonio et al, 2008;Genis et al, 2008Genis et al, , 2009Leroith et al, 2005;Molad et al, 2014;Nagano et al, 2013;Sivakumar et al, 2013b;Tattiyapong et al, 2014). Almost all these studies invariably focused on the genetic diversity of the MSA genes of B. bovis derived from cattle, whereas B. bovis in buffaloes has rarely been investigated, although this host species is considered to play a significant role in its epidemiology (da Silva et al, 2013;Elsify et al, 2015;He et al, 2012;Sivakumar et al, 2013a).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, these observations suggest that the levels of genetic diversity in the B. bovis MSA genes are similar in the cattle and water buffaloes of Vietnam. The MSA-2c gene is diverse in Australia (Berens et al, 2005), Sri Lanka (Sivakumar et al, 2013b), the Philippines (Tattiyapong et al, 2014), and Israel (Molad et al, 2014), whereas this gene is conserved in Mongolia (Altangeral et al, 2012) and Brazil (Ramos et al, 2012). In addition, the sequences isolated in Argentina, Mexico, and USA shared high similarities, suggesting that the MSA-2c from distant geographical locations may also sometimes be conserved (Dominguez et al, 2010;Florin-Christensen et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Studies conducted in Australia (Berens et al, 2005;Leroith et al, 2005), Sri Lanka (Sivakumar et al, 2013b), the Philippines (Tattiyapong et al, 2014), Thailand (Simking et al, 2013), Israel (Molad et al, 2014), Brazil (Nagano et al, 2013), and Mexico Genis et al, 2008Genis et al, , 2009) have found that B. bovis isolates in these countries are genetically diverse, according to their MSA gene sequences.…”

Section: Introductionmentioning

confidence: 99%

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Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Yokoyama

Sivakumar

Tuvshintulga

et al. 2015

Infection, Genetics and Evolution

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The genes that encode merozoite surface antigens (MSAs) in Babesia bovis are genetically diverse. In this study, we analyzed the genetic diversity of B. bovis MSA-1, MSA-2b, and MSA-2c genes in Vietnamese cattle and water buffaloes. Blood DNA samples from 258 cattle and 49 water buffaloes reared in the Thua Thien Hue province of Vietnam were screened with a B. bovis-specific diagnostic PCR assay. The B. bovis-positive DNA samples (23 cattle and 16 water buffaloes) were then subjected to PCR assays to amplify the MSA-1, MSA-2b, and MSA-2c genes. Sequencing analyses showed that the Vietnamese MSA-1 and MSA-2b sequences are genetically diverse, whereas MSA-2c is relatively conserved. The nucleotide identity values for these MSA gene sequences were similar in the cattle and water buffaloes. Consistent with the sequencing data, the Vietnamese MSA-1 and MSA-2b sequences were dispersed across several clades in the corresponding phylogenetic trees, whereas the MSA-2c sequences occurred in a single clade. Cattle- and water-buffalo-derived sequences also often clustered together on the phylogenetic trees. The Vietnamese MSA-1, MSA-2b, and MSA-2c sequences were then screened for recombination with automated methods. Of the seven recombination events detected, five and two were associated with the MSA-2b and MSA-2c recombinant sequences, respectively, whereas no MSA-1 recombinants were detected among the sequences analyzed. Recombination between the sequences derived from cattle and water buffaloes was very common, and the resultant recombinant sequences were found in both host animals. These data indicate that the genetic diversity of the MSA sequences does not differ between cattle and water buffaloes in Vietnam. They also suggest that recombination between the B. bovis MSA sequences in both cattle and water buffaloes might contribute to the genetic variation in these genes in Vietnam.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Yokoyama

Sivakumar

Tuvshintulga

et al. 2015

Infection, Genetics and Evolution

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“…The genetic diversity of msa (msa-1, msa-2b, and msa-2c) has been analyzed in several B. bovis-endemic countries in the recent past (Genis et al, 2009;Altangerel et al, 2012;Sivakumar et al, 2013). Studies conducted in Australia (Berens et al, 2005;LeRoith et al, 2005), Sri Lanka (Sivakumar et al, 2013), the Philippines (Tattiyapong et al, 2014), Thailand (Simking et al, 2013), Israel (Molad et al, 2014), Mexico (Borgonio et al, 2008;Genis et al, 2009) and Brazil (Nagano et al, 2013;Matos et al, 2017;Mendes et al, 2019) have found that B. bovis isolates in these countries are genetically diverse, according to their msa gene sequences.…”

Section: Discussionmentioning

confidence: 99%

“…The genetic diversity of B. bovis was previously shown based on MSA genes among cattle sampled in Australia (Berens et al, 2005;LeRoith et al, 2005), Brazil (Nagano et al, 2013;Matos et al, 2017;Simking et al, 2013;Mendes et al, 2019), Sri Lanka (Sivakumar et al, 2013), Philippines (Tattiyapong et al, 2014) and Israel (Molad et al, 2014). Antigenic variations arising from this genetic diversity result in different immune profiles in their hosts.…”

Section: Introductionmentioning

confidence: 95%

Genetic diversity of Babesia bovis studied longitudinally under natural transmission conditions in calves in the state of Rio de Janeiro, Brazil

Matos

Silva

Gonçalves

et al. 2020

Rev. Bras. Parasitol. Vet.

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Serum and DNA samples from 15 naturally infected calves in Seropédica, Brazil, were obtained quarterly from birth to 12 months of age, in order to longitudinally evaluate their humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis. Anti-B. bovis IgG antibodies were detected by an indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). Using DNA amplification, sequencing and phylogenetic analysis, the genetic diversity of B. bovis was assessed based on the genes that encode merozoite surface antigens (MSA-1, MSA-2b and MSA-2c). The serological results demonstrated that up to six months of age, all the calves developed active immunity against B. bovis. Among the 75 DNA samples evaluated, 0, 3 and 5 sequences of the msa-1, msa-2b and msa-2c genes were obtained, respectively. The present study demonstrated that the msa-2b and msa-2c gene sequences amplified from blood DNA of B. bovis-positive calves were genetically diversified. These data emphasize the importance of conducting deeper studies on the genetic diversity of B. bovis in Brazil, in order to design diagnostic antigens and vaccines in the future.

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Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil

et al. 2019

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Genetic polymorphism of Babesia bovis merozoite surface antigens-2 (MSA-2) isolates from bovine blood and Rhipicephalus annulatus ticks in Israel

Cited by 15 publications

References 34 publications

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Genetic diversity of Babesia bovis studied longitudinally under natural transmission conditions in calves in the state of Rio de Janeiro, Brazil

Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil

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