Genetic polymorphism of desialyzed alpha2 HS-glycoprotein by ultrathin isoelectric focusing

Sebetan, Ismail M.; Heshmat, Mona

doi:10.1007/bf00201115

Cited by 12 publications

(6 citation statements)

References 5 publications

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“…In the Asian populations the frequencies of AHSG*I tends to decrease from Japan to Thai (Umetsu et al, 1984;Umetsu et al, 1988b). The frequencies of AHSG*I in Sri Lankans are sim- (1989) Paraguayan (200) 1.0000 0 0 0 The present study (1987) ilar to those in Libyan (Sebetan and Heshmat, 1988) and Indian-Pakistani (Westwood et al, 1987a). These populations show the highest frequency for AHSG*I in all the populations studied thus far.…”

Section: A Hsg Polymorphismsupporting

confidence: 43%

Genetic polymorphisms of orosomucoid and alpha-2-HS-glycoprotein in Thai, Sri Lankan and Paraguayan populations

et al. 1989

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Section: A Hsg Polymorphismsupporting

confidence: 43%

Genetic polymorphisms of orosomucoid and alpha-2-HS-glycoprotein in Thai, Sri Lankan and Paraguayan populations

et al. 1989

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“…Native plasma was used for typing F13A, C6, C7, and APOC2 according to the techniques of Sebetan (1988), Nakamura et al (1984), and Sepehrnia et al (1988a). Plasma desialylated overnight at 25°C with neuraminidase (1 U/ml in 0.005 M sodium acetate buffer, pH 5.5) was used for typing F13B, ORMl, and AHSG after Nakamura et al (1986) and Yuasa et al (1987).…”

Section: Methodsmentioning

confidence: 99%

Survey of seven plasma protein polymorphisms in the Amhara and Oromo populations of Ethiopia

Scacchi¹,

Rm²,

Rickards

et al. 1994

American J Hum Biol

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The Ethiopian population is very difficult to specify due to a very high degree of intermixing among different peoples. The two groups of the present study, the Amhara and Oromo, constitute 38% and 35% of the population, respectively. In order to investigate the genetic composition of the Amhara and Oromo, genetic polymorphisms of seven plasma proteins (F13A, F13B, ORM1, AHSG, C6, C7, and APOC2), already identified as useful anthropological markers, were studied. No statistically relevant differences were found between the two groups for all of the systems examined. ORM1 and F13A showed frequencies in the range observed in other populations of Caucasoid and Negroid origin. F13B, AHSG, and C6 displayed gene frequencies and a number of variant alleles that seem particular to these two groups. No variation was observed for C7 and APOC2. Correspondence and distance analyses were used to interpret and compare the gene frequencies of the Amhara and Oromo with those of other related populations. These methods locate Ethiopians in an intermediate position between African Blacks and a group of Caucasoid populations, confirming cultural and historical data. © 1994 Wiley-Liss, Inc.

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“…Elevation of serum AHSG has been correlated with a common form of mild osteogenesis imperfecta where bone mass is lost (8). Homozygosity for AHSG*1, a polymorphism in the AHSG gene (9), is associated with shorter stature and reduced bone quality as measured by calcaneal broadband ultrasound (10). Fetuin does not appear to be required for embryogenesis, as mice lacking the protein are viable with no gross anatomical abnormality at birth (11).…”

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confidence: 99%

Regulation of Osteogenesis by Fetuin

Binkert¹,

Demetriou²,

Sukhu³

et al. 1999

Journal of Biological Chemistry

118

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Osteoporosis is a common problem of aging and results from a failure of homeostatic mechanisms to regulate osteogenesis and mineralization. Bovine and human forms of fetuin glycoprotein bind to the transforming growth factor (TGF)-␤/BMP (bone morphogenic protein) cytokines and block their osteogenic activity in cell culture assays (Demetriou, M., Binkert, C., Sukhu, B., Tenenbaum, H. C., and Dennis, J. W. (1996) J. Biol. Chem. 271, 12755-12761). Fetuin is a prominent serum glycoprotein and a major noncollagenous component of mineralized bone in mammals. In this study, we show that recombinant fetuin and native serum protein have similar potency as inhibitors of osteogenesis in dexamethasone-treated rat bone marrow cell cultures (dex-RBMC). Recombinant bovine fetuin also bound to TGF-␤1 and BMP-2 in vitro with kinetics similar to native fetuin. Although TGF-␤1 is required for osteogenesis in dex-RBMC, the cytokine also inhibited osteogenesis at concentrations >10 pM. Titration of fetuin or anti-TGF-␤1 antibodies into the bone marrow cultures in the presence of 10 pM TGF-␤1 restored osteogenesis, whereas titrations of the same reagents into cultures with 0.3 pM added TGF-␤1 were inhibitory, confirming the biphasic nature of the TGF-␤1 response. Suppression of osteogenesis by both TGF-␤1 and the antagonist proteins required their presence within the first 6 days of culture, well before mineralization at 10 -12 days. Northern analysis showed that both fetuin and high dose TGF-␤1 suppressed expression of the bone-associated transcripts alkaline phosphatase, osteopontin, collagen type I, and bone sialoprotein. The suppression of osteogenesis by fetuin and by high dose TGF-␤1 was accompanied by the differentiation of an alternate cell lineage with adipocyte characteristics. In summary, the biphasic osteogenic response to TGF-␤1 suggests that overlapping gradients of TGF-␤/BMP cytokines and fetuin regulate osteogenesis in remodeling bone.

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Genetic polymorphism of desialyzed alpha2 HS-glycoprotein by ultrathin isoelectric focusing

Cited by 12 publications

References 5 publications

Genetic polymorphisms of orosomucoid and alpha-2-HS-glycoprotein in Thai, Sri Lankan and Paraguayan populations

Genetic polymorphisms of orosomucoid and alpha-2-HS-glycoprotein in Thai, Sri Lankan and Paraguayan populations

Survey of seven plasma protein polymorphisms in the Amhara and Oromo populations of Ethiopia

Regulation of Osteogenesis by Fetuin

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