Genetic polymorphism of milk protein variants and their association studies with milk yield in Sahiwal cattle

Mir, Shahlla N.; Ullah, Obaid; Sheikh, Riazuddin

doi:10.5897/ajb2013.13216

Cited by 21 publications

(12 citation statements)

References 71 publications

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“…A2 β-casein variant is good for health as compared to A1 variant (Laugesen and Elliott, 2003). In a study conducted on sahiwal cattle breed of Pakistan population, A2 allelic variance was reported, similar to results of this study (Mir et al, 2014). Whereas, A1A1 and A1A2 genotypes of β-casein gene were detected in Holstein and native cattle breeds (Firouzamandi et al, 2018).…”

Section: Discussionsupporting

confidence: 88%

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Biodiversity of Soil Inhabiting Prostigmata (Arachnida: Acari) from Different Agro-Ecological Zones of Punjab, Pakistan

Khan¹,

Bashir²,

Ahmed³

et al. 2021

PJZ

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Authors' Contribution AN and MJ conceived the idea and executed the project. RBK carried out the genomic work. MZS and MEB assisted in data analysis and interpretation. RBK and MJ assisted in manuscript write up and editing. Key words β-casein, Cholistani cattle, Variants β-casein is second most abundant protein of cow's milk. β-casein gene is highly polymorphic. A1 and A2 are the frequently occurred variants. A1 is recognized as potential cause of several human diseases. It is important to evaluate the A1/A2 β-casein status in milk. Current study was conducted to molecular characterize the exonic regions of β-casein gene and to explore the status of A1/A2 β-casein type in Cholistani cattle breed of Pakistan. Blood samples of Cholistani Cattle were collected from Government Livestock Farm, Jugait Peer, Bahawalpur. Genomic DNA was extracted from whole blood by organic method. PCR primers were designed and optimized according to respective melting temperatures. PRALINE tool, MEGA 6.0 and POPGENE tool were utilized for phylogenetic tree construction and statistical analysis, respectively. Characterization of physical and chemical properties of β-casein protein was performed by ProtParam and SWISS MODEL was utilized for protein model prediction. Sequencing results of amplified PCR products revealed total 9 SNPs including 1 in exonic and 8 in intronic regions of β-casein gene. Results represent the presence of A2 allele in milk of Cholistani Cattle of Pakistan. Multiple sequence alignment represented the presence of MKVLILACLVALALARE and QRAVPVQALLLNQE as highly conserved regions. Phylogenetic analysis revealed the evolutionary relationship among Cholistani cattle of Pakistan, Bos indicus and Bos taurus. β-casein gene is highly polymorphic and A2 allele is present in Cholistani Cattle of Pakistan.

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Section: Discussionsupporting

confidence: 88%

“…A2 allelic frequency was higher in Holstein and Simmental breeds respectively whereas, with 0.5618 A1 allelic frequency was higher in Pinzgau breed (Miluchová et al, 2014). A2A2 and A2B genotypic frequencies of Sahiwal cattle were 0.14 and 0.86, respectively (Mir et al, 2014).…”

Section: O N L I N E F I R S T a R T I C L Ementioning

confidence: 95%

Biodiversity of Soil Inhabiting Prostigmata (Arachnida: Acari) from Different Agro-Ecological Zones of Punjab, Pakistan

Khan¹,

Bashir²,

Ahmed³

et al. 2021

PJZ

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“…Earlier reports of screening in Tharparkar cattle also showed similar allelic frequency of 0.05 and 0.95 for A1 and A2 allele respectively (Kumar et al 2018). The study on other indigenous breeds of cattle showed similar frequency of A2 allele viz., 0.94 in Ongole), 0.93 in Sahiwal and 0.94 in Gir cattle breeds, (Ganguly et al 2013, Mir et al 2014and Kumar et al 2018. Consistent with the present finding, Ramesha et al (2016) had also reported very low frequency of A1 allele in Malnad Gidda (0.014) and Kasargode (0.042) cattle.…”

Section: Resultsmentioning

confidence: 99%

Analysis of beta-casein gene (CSN2) polymorphism in Tharparkar and Frieswal cattle

Kumar

Singh

Chauhan

et al. 2019

IJAR

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A1 and A2 are most frequently observed Beta-Casein variants in dairy cattle. A1 â-casein on digestion releases an opioid, BCM-7 which has been found significantly associated with type 1 diabetes mellitus (DM-1), ischemic heart disease (IHD), autism and including other immune suppression activities in man. Whereas A2 â-casein is devoid of such adverse effects. The present study involved genotyping of 181 cattle of two breeds Viz., Tharparkar and Frieswal for genetic variants A1 and A2 beta casein gene by PCR-RFLP method using DdeI restriction enzyme. The results showed that the A1 and A2 allelic frequency as 0.04 and 0.96 in Tharparkar while in Frieswal (HF x Sahiwal), it was 0.37 and 0.63 respectively. The average genetic diversity (0.47), polymorphism information content (0.35) and effective allele number (1.87) reflected existence of medium genetic variability in tested population. The lower PIC value in Tharparkar cattle (0.073) indicates low genetic diversity whereas; moderate vale (0.35) in Frieswal cattle indicates medium genetic diversity. Higher fixation index value revealed that random mating is being practiced in both populations.

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“…No significant relationships were found between the protein content values and other genotypes. In the individual studies performed, while some authors found the differences among protein content means in the various BLG genotypes significant (Ojala et al, 2004;Felenczak et al, 2008;Mohammadi et al, 2013;Dyman et al, 2015), other authors did not find the differences significant (Eenennaam and Medrano, 1991;Ikonen et al, 1999;Botara et al, 2009;Molee et al, 2015).…”

Section: Traitsmentioning

confidence: 96%

“…Many studies look at the relationship between milk protein genetic variants and various milk production traits. Some authors reported the possibility of using milk protein as a polymorphic genetic marker (Eenennaam and Medrano, 1991;Chung et al, 1996;Kaygisiz and Dogan, 1999;Ikonen et al, 1999;Cardak, 2005;Alipanah et al, 2008;Heck et al, 2009;Mohammadi et al, 2013;Djedovic et al, 2015;Dyman et al, 2015). However, these relationships were reported to be non-significant by other researchers (Sekerden et al, 1999;Jusczak et al, 2001;Micinski et al, 2006;Ozdemir and Dogru, 2007;Gurcan, 2011;Dogru, 2015a;Molee et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Relationships between milk protein polymorphisms and production traits in cattle: a systematic review and meta-analysis

et al. 2018

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Abstract. Many researchers who have studied the relationships between milk protein polymorphisms and some yield traits in dairy cattle have reported incompatible results. In this study, in order to examine the overall relationships between milk protein genes known as major genes (CSN1S1, CSN2, CSN3, and BLG) and some yield traits (daily milk yield, lactation milk yield, fat yield, fat content, protein yield, and protein content), a meta-analysis was performed using some genetic models reported in the results of previous studies on cattle. The results suggest that the relationships of major milk protein genes with other factors should be studied using the codominant genetic model in general. Relationships among some CSN3 genotypes and fat yield, fat content, and protein content, and relationships between some BLG genotypes and daily milk yield, fat content, protein yield, and protein content were significant (P<0.05). No significant (P>0.05) relationships were found between these genotypes and other milk production traits. In addition, no significant (P>0.05) relationships between the CSN1S1 and CSN2 genotypes with the milk production traits examined were observed.

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Genetic polymorphism of milk protein variants and their association studies with milk yield in Sahiwal cattle

Cited by 21 publications

References 71 publications

Biodiversity of Soil Inhabiting Prostigmata (Arachnida: Acari) from Different Agro-Ecological Zones of Punjab, Pakistan

Biodiversity of Soil Inhabiting Prostigmata (Arachnida: Acari) from Different Agro-Ecological Zones of Punjab, Pakistan

Analysis of beta-casein gene (CSN2) polymorphism in Tharparkar and Frieswal cattle

Relationships between milk protein polymorphisms and production traits in cattle: a systematic review and meta-analysis

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