Genetic polymorphism of tear proteins in the rat

Abstract

Polymorphism of tear proteins was found by agarose gel electrophoresis among inbred strains of rats. The proteins (RTP-1) are inherited as a single autosomoal trait. The locus was designated Rtp-1 (rat tear protein-1) and it had two codominant alleles (Rtp-1a, Rtp-1b). Although we did not find any recombinant between the Rtp-1 and the Mup-1 loci among 67 backcross progeny, we found 3 strains with the recombinant type between them in 33 inbred strains tested. The results suggest that the Rtp-1 locus is very closely linked with the Mup-1 locus, which belongs to rat linkage group II. RTP-1 proteins strongly reacted with anti-MUP-1 A serum on agarose gel electrophoretograms.

Second Genetic Polymorphism of Tear Proteins in the Rat (<I>Rtp-2</I>)

“…In the previous study [10], agarose gel electrophoresis of rat tear proteins in the middle anodic zone revealed the presence of genetic polymorphism.…”

“…We designated the locus determining this component as Rtp-1 (rat tear protein-1), which is tightly linked to the Mup-1 locus in linkage group II [10]. We also reported the fastest migrating tear protein was not detectable only in the SHR strain among ten inbred strains [9].…”

“…To further confirm the polymorphism, we clarified the mode of inheritance, strain distributions of the alleles at the locus, and tested the linkages of this new locus to a few coat color genes, several biochemical gene loci, and the PTI (major histocompatibility complx of the rat) by mating experiments between SHR and ACI strains of the rat. Electrophoretic methods were the same as previously reported [10]. Briefly, electrophoresis was carried out on 1.4% thin (1 mm Azen [1] found an electrophretic polymorphism in the fastest migrating protein (At protein) in human tears.…”

Studies on the Biochemical Marker Loci Detected with Urine, Tears, and Saliva of Rats

“…These were designated mouse tear protein system 1, 2, 3, and 4 (Mtp-1, 2, 3, and 4), [31,33,34,35]. Using agarose gel electrophoresis, we could find two polymorphisms were found in the tear proteins of inbred strains of rats [24,25,26].…”