Genetic polymorphism rs3760396 of the chemokine (C-C motif) ligand 2 gene (CCL2) associated with the susceptibility of lung cancer in a pathological subtype-specific manner in Han-ancestry Chinese: a case control study

Xu, Lisha; Lin, Fang‐Yue; Zhou, Hong

doi:10.1186/s12885-016-2328-8

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…42,43 The chemokine CCL2 has been identified to be an important characteristic substance in many human cancers such as breast cancer, 44 ovarian cancer, 45 and lung cancer. 46 According to previous researches, the polymorphisms of CCL2 and its receptors are related to the transitional bladder cell carcinoma. 47,48 A few studies have also reported that CCL2 is a key contributor in mediating the migration and invasion of BC cells in the signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression

et al. 2017

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We attempted to analyze the effects of miR-1-3p and CCL2 on the proliferation, migration, and invasion of bladder cancer cells. A total of 18 pairs of bladder cancer tissues with corresponding adjacent tissues and the 6 cases of normal tissues were collected. The expressions of miR-1-3p and CCL2 in the cancer tissues were evaluated using quantitative real-time polymerase chain reaction and western blot. The relationship between miR-1-3p and CCL2 was assessed using luciferase reporter assay. The UM-UC-3 bladder cancer cells were transfected with CCL2 small interfering RNA and miR-1-3p mimics. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, wound healing assay, Transwell assay, and the flow cytometry test were used to detect the proliferation, migration, invasion, and apoptosis of bladder cancer cells. Bladder cancer tissues had lower levels of miR-1-3p but higher levels of CCL2 than normal tissues (p < 0.05). The transfection of miR-1-3p mimics and CCL2 small interfering RNA remarkably suppressed cell proliferation and invasion and promoted apoptosis of cells (p < 0.05). Results of the luciferase reporter gene assay demonstrated that miR-1-3p targeted CCL2. MiR-1-3p suppresses the proliferation and invasion of urinary bladder cancer cells by targeting CCL2.

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Section: Discussionmentioning

confidence: 99%

MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression

et al. 2017

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“…Rs1024611 seats on positions -2518 in MCP-1 5'-flnking region with the allele mutation of A to G, and affects MCP-1 transcription activity [15]. Rs3760396 is confirmed as a deleterious mutation in the Chinese Han population, and has been reported to be correlated with lung cancer [16]. However, their genetic association with AMD risk in the Chinese Han population has been unclear.…”

Section: Introductionmentioning

confidence: 99%

Association Between Monocyte Chemotactic Protein 1 Variants and Age-Related Macular Degeneration Onset Among Chinese People

Zhang

Zhao

2020

Med Sci Monit

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Departmental sources Background:We assessed the potential association between monocyte chemotactic protein 1 (MCP-1) variants (rs1024611 and rs3760396) and age-related macular degeneration (AMD) susceptibility among Chinese Han people. Material/Methods:Our research included 129 AMD patients and 131 healthy controls. Genotyping for MCP-1 variants was performed in the 2 groups, and genotype and allele distributions were checked between groups by c 2 analysis. Odds ratio (OR) and 95% confidence interval (CI) reflected the potential association between MCP-1 variants and AMD risk. The linkage disequilibrium of polymorphisms was detected using Haploview. Results:Significant differences in rs1024611 genotype distributions were detected between the 2 groups, and homozygous carriers with GG genotype had higher AMD incidence (P<0.05, OR=2.650, 95% CI=1.127 -6.231). The rs1024611 G allele frequency was significantly higher in AMD patients, suggesting that the G allele promotes AMD onset (P<0.05, OR=1.447, 95% CI=1.013 -2.068). Strong linkage disequilibrium was found between rs1024611 and rs3760396, and haplotype A rs1024611 -C rs3760396 was significantly associated with decreased risk of AMD (P=0.001, OR=0.502, 95% CI=0.335 -0.752). Conclusions:MCP-1 rs1024611 variant appears to contribute to risk of AMD in the Chinese Han population, and the interaction of MCP-1 polymorphisms may also influence individual susceptibility to AMD.

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“…The difference between the average expression level of this gene in the cancer samples and its average expression level in the normal samples was as large as 1678.72, whereas the average of the corresponding differences for all the DE genes identified by SAM was only 245.03. It has been reported that this gene may play an important role in the development of lung cancer29. For another example, the average expression level of TNFAIP3 was ranked at the top 7.4% and 3.2% of all the measured genes in the cancer and normal samples, respectively.…”

Section: Resultsmentioning

confidence: 99%

Identifying reproducible cancer-associated highly expressed genes with important functional significances using multiple datasets

Huang

Guo

et al. 2016

Sci Rep

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Identifying differentially expressed (DE) genes between cancer and normal tissues is of basic importance for studying cancer mechanisms. However, current methods, such as the commonly used Significance Analysis of Microarrays (SAM), are biased to genes with low expression levels. Recently, we proposed an algorithm, named the pairwise difference (PD) algorithm, to identify highly expressed DE genes based on reproducibility evaluation of top-ranked expression differences between paired technical replicates of cells under two experimental conditions. In this study, we extended the application of the algorithm to the identification of DE genes between two types of tissue samples (biological replicates) based on several independent datasets or sub-datasets of a dataset, by constructing multiple paired average gene expression profiles for the two types of samples. Using multiple datasets for lung and esophageal cancers, we demonstrated that PD could identify many DE genes highly expressed in both cancer and normal tissues that tended to be missed by the commonly used SAM. These highly expressed DE genes, including many housekeeping genes, were significantly enriched in many conservative pathways, such as ribosome, proteasome, phagosome and TNF signaling pathways with important functional significances in oncogenesis.

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Genetic polymorphism rs3760396 of the chemokine (C-C motif) ligand 2 gene (CCL2) associated with the susceptibility of lung cancer in a pathological subtype-specific manner in Han-ancestry Chinese: a case control study

Cited by 6 publications

References 21 publications

MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression

MiR-1-3p inhibits the proliferation and invasion of bladder cancer cells by suppressing CCL2 expression

Association Between Monocyte Chemotactic Protein 1 Variants and Age-Related Macular Degeneration Onset Among Chinese People

Identifying reproducible cancer-associated highly expressed genes with important functional significances using multiple datasets

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