Genetic polymorphisms in TLR1, TLR2, TLR4, and TLR10 of Helicobacter pylori-associated gastritis: a prospective cross-sectional study in Thailand

Tongtawee, Taweesak; Bartpho, Theeraya Simawaranon; Kaewpitoon, Soraya J; Kaewpitoon, Natthawut; Dechsukhum, Chavaboon; Leeanansaksiri, Wilairat; Loyd, Ryan A; Talabnin, Krajang; Matrakool, Likit; Panpimanmas, Sukij

doi:10.1097/cej.0000000000000347

Cited by 34 publications

(14 citation statements)

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“…Association between TRL1 rs4833095 and tuberculosis susceptibility [36, 37], increased risk of IgA nephropathy [38], Crohn's disease and ulcerative colitis [39], susceptibility for alopecia areata [40], increased mortality in patients with sepsis following traumatic injury [35] have been found in various studies. The discovery of association between TLR1 SNP rs4833095 and H. pylori seroprevalence in a GWAS in European descent population [20] was replicated in the Thai population: the TLR1 rs4833095, C allele was associated with a significantly increased risk for H. pylori infection [19]. Moreover, the above mentioned SNP along with TLR10 rs10004195 was associated with GC in H. pylori infected subjects in a Malaysian population [18].…”

Section: Discussionmentioning

confidence: 86%

“…The PRRs recognize bacterial lipopolysaccharides (LPS) and induce the secretion of pro-inflammatory molecules [18]. Polymorphisms of TLR genes can confer host susceptibility to H. pylori infection as well as to H. pylori related diseases by modulating the release of pro-inflammatory cytokines, which underline gastric inflammation and the immune response to H. pylori [19]. A recent genome-wide association study (GWAS) revealed significant association of single-nucleotide polymorphisms (SNPs) in the TRL1 gene with susceptibility of H. pylori in an European descent population [20].…”

Section: Introductionmentioning

confidence: 99%

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TLR1 and PRKAA1 Gene Polymorphisms in the Development of Atrophic Gastritis and Gastric Cancer

Dargienė

Streleckiene

Skiecevičienė³

et al. 2018

JGLD

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Background & Aims: Previous genome-wide association studies showed that genetic polymorphisms in toll-like receptor 1 (TLR1) and protein kinase AMP-activated alpha 1 catalytic subunit (PRKAA1) genes were associated with gastric cancer (GC) or increased Helicobacter pylori (H. pylori) infection susceptibility. The aim of this study was to evaluate the association between TLR1 and PRKAA1 genes polymorphisms and H.pylori infection, atrophic gastritis (AG) or GC in the European population.Methods: Single-nucleotide polymorphisms (SNPs) were analysed in 511 controls, 340 AG patients and 327 GC patients. TLR1 C>T (rs4833095) and PRKAA1 C>T (rs13361707) were genotyped by the real-time polymerase chain reaction. H. pylori status was determined by testing for anti-H. pylori IgG antibodies in the serum.Results: The study included 697 (59.2%) H. pylori positive and 481 (40.8%) H. pylori negative cases. We observed similar distribution of TLR1 and PRKAA1 alleles and genotypes in H. pylori positive and negative cases. TLR1 and PRKAA1 SNPs were not linked with the risk of AG. TC genotype of TLR1 gene was more prevalent in GC patients compared to the control group (29.7% and 22.3% respectively, p=0.002). Carriers of TC genotype had a higher risk of GC (aOR=1.89, 95% CI: 1.26–2.83, p=0.002). A similar association was observed in a dominant inheritance model for TLR1 gene SNP, where comparison of CC+TC vs. TT genotypes showed an increased risk of GC (aOR=1.86, 95% CI: 1.26–2.75, p=0.002). No association between genetic polymorphism in PRKAA1 gene and GC was observed.Conclusions: TLR1 rs4833095 SNP was associated with an increased risk of GC in a European population, while PRKAA1 rs13361707 genetic variant was not linked with GC. Both genetic polymorphisms were not associated with H. pylori infection susceptibility or the risk of AG.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

TLR1 and PRKAA1 Gene Polymorphisms in the Development of Atrophic Gastritis and Gastric Cancer

Dargienė

Streleckiene

Skiecevičienė³

et al. 2018

JGLD

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“… 18 TLR10 (rs10004195) is linked with infection susceptibility, where the frequency of the TT genotype is reported to be 66% in H pylori- positive cases from a Chinese population. 29 Therefore, the T allele of TLR10 rs10004195 polymorphism showed an increased risk of chronic atrophic gastritis.…”

Section: Discussionmentioning

confidence: 99%

Association of toll-like receptor 4, 5 and 10 polymorphisms with Helicobacter pylori -positive peptic ulcer disease in a center in Jordan

et al. 2021

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BACKGROUND: Helicobacter pylori infection is widespread, affecting about 50% of the global population. Polymorphisms in host genes such as the toll-like receptor 4 ( TLR4 ) might affect the susceptibility and severity of infection and treatment success. OBJECTIVE: Investigate the susceptibility and severity of H pylori infection with host TLR4 (rs11536889, rs4986790, rs200109652, rs10759932), TLR5 (rs5744174, rs2072493, rs746250566), TLR10 (rs559182335, rs10004195) polymorphisms. DESIGN: Analytical, cross-sectional. SETTING: Endoscopy clinic at tertiary care center. PATIENTS AND METHODS: Genomic DNA was extracted from formalin-fixed paraffin-embedded tissues collected from H pylori -infected patients and healthy individuals. The single nucleotide polymorphisms (SNPs) within the targeted TLR genes were genotyped to assess the genetic association of various SNPs with disease severity. MAIN OUTCOME MEASURES: Effect of genotype distribution on H pylori infection. SAMPLE SIZE: 250 peptic ulcer patients and 217 controls. RESULTS: The TLR10 genotype showed no significant association with H pylori infection except for rs10004195 (T>A) ( P =.002). The genotype frequency of Rs5744174 in TLR5 had a significant association with the presence of H pylori infection ( P =.046, OR=0.52). Except for gender (P=.022), there were no significant associations between clinical and demographic variables and SNPs relating to the severity of the H pylori infections. CONCLUSIONS: Our findings are consistent with differences in severity of H pylori infection due to TLR SNPs in different ethnic groups. Understanding differences in genetic susceptibility could help in classifying patients and matching patients with various treatment options on a genetic basis. LIMITATIONS: Lack of H pylori pathogenicity features assessment. CONFLICTS OF INTEREST: None.

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“…Recently, the TLR1 rs4833095 CC homozygous wild type and the TLR10 rs10004195 AA homozygous wild type were associated with H. pylori susceptibility and increased risk of H. pylori infection in chronic gastritis patients (25). Moreover, TLR10 polymorphism was associated with H. pyloriinduced inflammation with gastric mucosal patterns, suggesting that the AA homozygous wild type contributes to severe inflammation in H. pylori-associated gastritis (26).…”

Section: Discussionmentioning

confidence: 99%

Toll-Like Receptors are Associated with Helicobacter pylori Infection and Gastric Mucosa Pathology

Simawaranon

Wattanawongdon

Tongtawee

2017

Jundishapur J Microbiol

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Background: Toll-like receptors (TLRs) mediate recognition of Helicobacter pylori and initiate the innate immune response to infection. Toll-like receptors polymorphisms are thought to influence susceptibility to H. pylori infection and H. pylori-related diseases. Objectives: To investigate the association of TLR polymorphisms with the susceptibility to H. pylori infection and various types of gastritis in Thai patients. Methods: The single nucleotide polymorphisms (SNPs) TLR1 rs4833095, TLR2 rs3804099 and rs3804100, TLR4 rs10759932 and TLR10 rs10004195 were analysed in 400 gastritis patients by real-time PCR using a TaqMan SNP Genotyping Assay. The association between TLR polymorphisms and the risk of H. pylori infection was investigated in 196 uninfected and 204 H. pylori-infected patients. The associations between TLR genotypes and the risk of gastric pathology changes, including chronic gastritis (N = 312 cases) and precancerous gastric lesions/gastric cancer (GC) (N = 88 cases), were examined. Univariate analysis was used to determine odds ratios (ORs) with confidence intervals (95% CIs). Results: Patients with the CC -or TT homozygous genotype for TLR1 rs4833095 had a significantly increased risk of H. pylori susceptibility (OR = 2.28, 95% CI = 1.27 -7.60, P = 0.02 and OR = 1.34, 95% CI = 0.97 -1.86, P = 0.04, respectively). Patients with the AA homozygous genotype for TLR10 rs10004195 had a significantly increased risk of H. pylori susceptibility (OR = 1.28, 95% CI = 0.98 -1.76, P = 0.01) and exhibited a significantly increased risk of precancerous gastric lesions/GC (OR = 8.75, 95% CI = 2.78 -14.24, P < 0.01). Conclusions: Our findings suggest that the TLR1 rs4833095 and TLR10 rs10004195 polymorphisms are potential genetic risk factors that modify the H. pylori infection susceptibility and influence the clinical manifestation of chronic gastritis, precancerous gastric lesions and GC in Thai patients.

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Genetic polymorphisms in TLR1, TLR2, TLR4, and TLR10 of Helicobacter pylori-associated gastritis: a prospective cross-sectional study in Thailand

Cited by 34 publications

References 21 publications

TLR1 and PRKAA1 Gene Polymorphisms in the Development of Atrophic Gastritis and Gastric Cancer

TLR1 and PRKAA1 Gene Polymorphisms in the Development of Atrophic Gastritis and Gastric Cancer

Association of toll-like receptor 4, 5 and 10 polymorphisms with Helicobacter pylori -positive peptic ulcer disease in a center in Jordan

Toll-Like Receptors are Associated with Helicobacter pylori Infection and Gastric Mucosa Pathology

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