Genetic Polymorphisms of Four Egyptian Plant Species using some Molecular Markers Techniques RAPD, ISSR, and SCoT

Ahmed, Amina; Soliman, Khaled A.; Al-Senosy, Neima K.; Omar, S.

doi:10.21608/jacb.2022.118365.1018

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…The above-obtained results suggested that the SCoT marker can be used to effectively detect the molecular alterations among treated spinach plants. These results are in harmony with previously obtained in Mangiferea indica (Luo et al 2010 ), in Arachis hypogaea (Xiong et al 2011 ), in Echinacea (Jedrzejczyk 2020 ), in Vitis uinifera (Ahmed et al 2021 ), in Lamiaceae species (Ahmed et al 2022 ).…”

Section: Discussionsupporting

confidence: 91%

Changes in morphological traits, anatomical and molecular alterations caused by gamma-rays and zinc oxide nanoparticles in spinach (Spinacia oleracea L.) plant

Aly,

Safwat,

Eliwa

et al. 2023

Biometals

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Spinach seeds were irradiated with gamma-rays after that soaked in zinc oxide nanoparticles (ZnO–NPs) at 0.0, 50, 100 and 200 ppm for twenty-four hours at room temperature. Vegetative plant growth, photosynthetic pigments, and proline contents were investigated. Also, anatomical studies and the polymorphism by the SCoT technique were conducted. The present results revealed that the germination percentage was at the maximum values for the treatment of 100 ppm ZnO–NPs (92%), followed by 100 ppm ZnO–NPs + 60 Gy (90%). The application of ZnO–NPs resulted in an enhancement in the plant length. The maximum of chlorophylls and carotenoids content was recorded in the treatment, 100 ppm ZnO–NPs + 60 Gy. Meanwhile, the irradiation dose level (60 Gy) with all ZnO–NPs treatments increased proline content and reached its maximum increase to 1.069 mg/g FW for the treatment 60 Gy combined with 200 ppm ZnO–NPs. Also, the anatomical studies declared that there were variations between the treatments; un-irradiated and irradiated combined with ZnO–NPs plants which reveal that the leave epidermal tissue increased with 200 ppm ZnO–NPs in both the upper and lower epidermis. While irradiated plants with 60 Gy combined with 100 ppm ZnO–NPs gave more thickness of upper epidermis. As well as SCoT molecular marker technique effectively induced molecular alterations between the treatments. Where, SCoT primers targeted many new and missing amplicons that are expected to be associated with the lowly and highly expressed genes with 18.2 and 81.8%, respectively. Also, showed that the soaking in ZnO-NPs was helped for reducing molecular alteration rate, both spontaneous and induced by gamma irradiation. This nominates ZnO–NPs as potential nano-protective agents that can reduce irradiation-induced genetic damage.

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Section: Discussionsupporting

confidence: 91%

Changes in morphological traits, anatomical and molecular alterations caused by gamma-rays and zinc oxide nanoparticles in spinach (Spinacia oleracea L.) plant

Aly,

Safwat,

Eliwa

et al. 2023

Biometals

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“…The rationale for the use of RAPD molecular markers for Lamiaceae species is supported by several current studies (Chowdhury et al, 2017;Ahmed and Al-Sodany, 2019;Saha et al, 2020;Sunar et al, 2020;Zhend et al, 2021;Ahmed et al, 2022).…”

Section: Resultsmentioning

confidence: 92%

Lavandula spp. Diversity Assessment by Molecular Markers as a Tool for Growers

Ražná

2023

Agrobiodivers Improv Nutr health life Qual

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Lavender is one of the most important medicinal plants. The quality and the therapeutic properties of the essential oil of lavender are determined by the quantity and the biological activity of the individual components. Different lavender varieties often have a characteristic profile, differing in their individual substances, with small differences affecting the aroma and properties of the essential oil. Genetic variation between species and cultivars, as well as environmental conditions, nutrition, season, and type of tissue, all influence the chemical composition of lavender essential oil. The collection of twelve genotypes of Lavandula spp., which includes species of L. angustifolia (Mill.), and Lavandula × intermedia Emeric ex Loisel. were grown in the locality of Malé Leváre (Slovakia). These species, without further specification of varieties, were screened for DNA polymorphism by random amplified polymorphic DNA (RAPD) markers. Leaf and flower samples were analyzed using 4 universal random decamers. Two decamers (OPB 11 and OPB 18) provided efficient and reproducible amplification profiles. Individual genotypes and leaf and flower samples were characterized using DNA fingerprint cards containing digital electrophoretic profiles generated by random decamer primers and the corresponding image of the lavender genotype. This low-cost and effective approach to genetic diversity screening can provide useful documentation for lavender growers and additional genotype specifications.

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Genetic Polymorphisms of Four Egyptian Plant Species using some Molecular Markers Techniques RAPD, ISSR, and SCoT

Cited by 2 publications

References 5 publications

Changes in morphological traits, anatomical and molecular alterations caused by gamma-rays and zinc oxide nanoparticles in spinach (Spinacia oleracea L.) plant

Changes in morphological traits, anatomical and molecular alterations caused by gamma-rays and zinc oxide nanoparticles in spinach (Spinacia oleracea L.) plant

Lavandula spp. Diversity Assessment by Molecular Markers as a Tool for Growers

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