Genetic Polymorphisms of Matrix Metalloproteinases 1–3 and Their Inhibitor are Not Associated With Premature Labor

Lathouras, Konstantinos; Saso, Srdjan; Tzafetas, Menelaos; Kalinderi, Kallirroi; Fidani, Styliani; Zournatzi, V.; Kyrgiou, Maria; Fotopoulou, Christina; Ghaem‐Maghami, Sadaf; Tzafetas, I.

doi:10.4155/fsoa-2018-0047

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…This can lead to amniotic fluid leakage, increasing the risk of fetal membrane infection and premature birth. AFC are an important component in maintaining the strength and elasticity of fetal membranes [ 28 ], and the early or excessive maturation of AFC can affect the stability of membranes. The functional assay of AFC after stimulation with PROZ illustrated that the PROZ concentration is positively associated with AFC proliferation and migration.…”

Section: Discussionmentioning

confidence: 99%

Amniotic Fluid Proteomics Analysis and In Vitro Validation to Identify Potential Biomarkers of Preterm Birth

Chen,

Wang,

Yan

et al. 2024

Reprod. Sci.

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This study aimed to investigate the regulation of amniotic fibroblast (AFC) function by vitamin K–dependent protein Z (PROZ) during preterm birth (PTB) and its potential role in adverse pregnancy outcomes. Proteomic samples were collected from amniotic fluid in the second trimester, and AFC were isolated from the amniotic membrane and cultured in vitro. The expression of extracellular and intracellular PROZ in AFC was modulated, and their biological properties and functions were evaluated. Clinical analysis revealed a significant upregulation of PROZ expression in amniotic fluid from preterm pregnant women. In vitro experiments demonstrated that PROZ stimulated AFC migration, enhanced their proliferative capacity, and reduced collagen secretion. Overexpression of PROZ further enhanced cell migration and proliferation, while knockdown of PROZ had the opposite effect. PROZ plays a crucial role in promoting the proliferation and migration of amniotic membrane fibroblasts. Increased PROZ expression in amniotic fluid is associated with the occurrence of PTB. These findings shed light on the potential involvement of PROZ in adverse pregnancy outcomes and provide a basis for further research on its regulatory mechanisms during PTB.

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Section: Discussionmentioning

confidence: 99%

Amniotic Fluid Proteomics Analysis and In Vitro Validation to Identify Potential Biomarkers of Preterm Birth

Chen,

Wang,

Yan

et al. 2024

Reprod. Sci.

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“…On the contrary, Fujimoto et al explored the same variant and found a significant association between fetal carriage of the 2G allele and preterm premature rupture of membranes [27]. In another study, Lathouras et al reported that different polymorphisms of MMP-1, -3 and TIMP-2 were not associated with premature labor [28].…”

Section: Discussionmentioning

confidence: 99%

“…The studied polymorphisms were genotyped through polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. The primers and restriction enzymes (Thermo Fisher Scientific; Waltham, MA, USA) employed in these reactions were sourced from previously published studies and are detailed in Table 7 [23,25,28,31,34]. Subsequently, DNA fragments were examined by electrophoresis on agarose gels using Midori Green Advance DNA Stain (Nippon Genetics, Düren, Germany).…”

Section: Studied Genetic Variantsmentioning

confidence: 99%

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Comprehensive Analysis of the Role of Gene Variants in Matrix Metalloproteinases and Their Tissue Inhibitors in Retinopathy of Prematurity: A Study in the Polish Population

Choręziak-Michalak,

Szpecht,

Chmielarz-Czarnocińska

et al. 2023

IJMS

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This study was designed to investigate the relationship between variants of matrix metalloproteinases (MMP-1 rs179975, MMP-9 rs17576 and rs17577), their tissue inhibitors (TIMP-1 rs4898, TIMP-2 rs2277698 and rs55743137) and the development of retinopathy of prematurity (ROP) in infants from the Polish population. A cohort of 100 premature infants (47% female) was enrolled, including 50 ROP cases and 50 no-ROP controls. Patients with ROP were divided into those with spontaneous remission and those requiring treatment. A positive association between MMP-1 rs179975 1G deletion allele and ROP was observed in the log-additive model (OR = 5.01; p = 0.048). Furthermore, female neonates were observed to have a negative association between the TIMP-1 rs4898C allele and the occurrence of ROP and ROP requiring treatment (codominant models with respective p-values < 0.05 and 0.043). Two and three loci interactions between MMP-1 rs1799750 and TIMP1rs4989 (p = 0.015), as well as MMP-1 rs1799750, MMP-9 rs17576 and TIMP-1 rs4989 (p = 0.0003) variants influencing the ROP risk were also observed. In conclusion, these findings suggest a potential role of MMPs and TIMPs genetic variations in the development of ROP in the Polish population. Further studies using a larger group of premature infants will be required for validation.

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Maternal genetic risk factors for spontaneous preterm birth: A systematic review and meta‐analysis

Mladenić,

Barišić,

Pereza

et al. 2024

Intl J Gynecology & Obste

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BackgroundDespite various genomic approaches used in prior studies investigating the association of maternal genetic variability with spontaneous preterm birth (sPTB), results show inconsistency and contradictions.ObjectivesTo conduct a systematic review of studies analyzing the association between maternal genetic variants and sPTB, evaluate retrieved studies based on selection criteria, classify studies into hypothesis‐based and hypothesis‐free, and perform a meta‐analysis to identify the strongest associations.Search strategyPubMed, Scopus, and reference lists were searched until October 2024.Selection criteriaEnglish‐language, case–control, cross‐sectional, and prospective cohort studies examining the association between maternal genetic variations and sPTB were included.Data collection and analysisData on authors, publication year, ethnicity, genes/variants, P values, study type, sample size, inclusion criteria, and methods were collected. The association strength was estimated using odds ratios with 95% confidence intervals.ResultsEighty‐one studies met eligibility criteria: 73 utilized a hypothesis‐based and 14 a hypothesis‐free approach. Thirty‐five studies qualified for a meta‐analysis, revealing a significant association in tumor necrosis factor α (rs1800629) gene for alleles and additive and recessive genetic models (P ≤ 0.05). From the hypothesis‐free approach, 13 genes reached global significance in association with sPTB (P < 5 × 10−8).ConclusionsNo single gene or variant was consistently associated with sPTB risk among studies. Hypothesis‐based analyses highlighted tumor necrosis factor α (rs1800629) as a modest signal, while hypothesis‐free approaches identified 13 genes with genome‐wide significance, pointing to new research directions in understanding sPTB genetics.

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Genetic Polymorphisms of Matrix Metalloproteinases 1–3 and Their Inhibitor are Not Associated With Premature Labor

Cited by 4 publications

References 21 publications

Amniotic Fluid Proteomics Analysis and In Vitro Validation to Identify Potential Biomarkers of Preterm Birth

Amniotic Fluid Proteomics Analysis and In Vitro Validation to Identify Potential Biomarkers of Preterm Birth

Comprehensive Analysis of the Role of Gene Variants in Matrix Metalloproteinases and Their Tissue Inhibitors in Retinopathy of Prematurity: A Study in the Polish Population

Maternal genetic risk factors for spontaneous preterm birth: A systematic review and meta‐analysis

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