Genetic polymorphisms of twelve X-STRs of the investigator Argus X-12 kit and additional six X-STR centromere region loci in an Egyptian population sample

Elakkary, Sally; Hoffmeister-Ullerich, S; Schulze, Christian; Seif, Eman; Sheta, Abeer; Hering, Sandra; Edelmann, Jeanett; Augustin, Christa

doi:10.1016/j.fsigen.2014.02.007

Cited by 38 publications

(26 citation statements)

References 26 publications

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“…The observed number of different haplotypes was 156, 115, 114 and 142 for LG1, LG2, LG3 and LG4, respectively (Table 1). Similar to other population studies [13,15,18], the linkage group I was the most polymorphic and linkage groups II and III were least informative showing each 71 unique haplotypes. The most common haplotype was observed 10 times in the LG3, corresponding to a population frequency of 5.0%.…”

Section: Resultssupporting

confidence: 86%

“…Twelve off-ladder alleles in the linkage groups I, II and IV -all reported so far [2,11,13,15,17] except for allele 26 of DXS10079 locus -were observed and designated according to their sizes (Table 3). A lower amplification efficiency at the DXS10103 locus for most of the sample tested was observed.…”

Section: Resultsmentioning

confidence: 99%

“…Allele and haplotype frequencies for the 12 X-STRs and for each linkage group (LG), as well as the pairwise genetic distances (Fst) based on haplotype frequencies, were calculated by using the Arlequin software v3.5 [9] between our Italian sample and a set of both neighboring and geographically distant populations. To maximize the effectiveness of the comparisons, only populations typed for all the same X-STR loci implemented in the Argus X-12 system were considered, for a total of 4 European (Germany [2], Denmark [10], Czech Republic [11] and Sweden [12]), 4 African (Egypt [13], Somalia [10], Ivory Coast [14] and Cabo Verde [15]) and 3 Asian (China and Japan [16], Malaysia [17]) populations. Inter-population genetic distances (Fst) between the 12 considered populations for each of the four linkage groups (LG) were integrated and graphically represented by using the R software package DISTATIS [5].…”

Section: Amplification and Data Analysismentioning

confidence: 99%

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Expanding X-chromosomal forensic haplotype frequencies database: Italian population data of four linkage groups

Bini¹,

Riccardi²,

Ceccardi³

et al. 2015

Forensic Science International: Genetics

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Amplification and Data Analysismentioning

confidence: 99%

See 1 more Smart Citation

Expanding X-chromosomal forensic haplotype frequencies database: Italian population data of four linkage groups

Bini¹,

Riccardi²,

Ceccardi³

et al. 2015

Forensic Science International: Genetics

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“…To our knowledge, this is the first report from Saudi Arabia on these 12 markers, so no Saudi data is available for comparison. In the present study, Saudi allele frequencies were compared with eight worldwide populations from the literature to elucidate the genetic relationships between Saudis, Emiratis [31], Egyptians [32], Turkish [33], Algerians [34], Jewish [35], Filipino [36], Bangladeshis [37] and Indians [38] using the population pairwise genetic distance fixation index (Fst). Fst was used to perform the comparison, with differences being statistically significant for p-values < 0.001 after Bonferroni correction ( Table 5).…”

Section: Interpopulation Diversitymentioning

confidence: 99%

“…Neighbouring joining tree based on Nei's Fst Distances for the 12 X-STR loci estimated among the 9 populations[31][32][33][34][35][36][37][38] . The nods are bootstrap values derived from 1000 replications.…”

mentioning

confidence: 99%

Genetic diversity and haplotypic structure of a Saudi population sample using Investigator Argus X-12 amplification kit

Messaoudi

Babu

Alsaleh

et al. 2019

Preprint

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Background:X-chromosome short tandem repeat (X-STR) markers have shown a great capability in forensic identity investigations and paternity testing involving kinship analysis. Material and methods:In the current study, the distribution of 12 X-STR loci (DXS10148, and HPRTB) located in four linkage groups (LG)s was evaluated using Investigator ® Argus X-12 Amplification Kit in 200 unrelated healthy individuals (105 males, and 95 females) from the central region of the Saudi Arabia in order to create a DNA database. Results:No significant difference was recorded in the allele frequencies of males and females.Our results indicated that DXS10146 locus was the most informative with 21 alleles while DXS8378 locus was the least with 5 alleles. Hardy-Weinberg equilibrium (HWE) was applied and confirmed for all loci in the female samples, except for DXS10074, DXS10101, DXS10135 and DXS10148 (p > 0.05/12 = 0.0042). Forensic parameters showed that all X-STRs loci either as individual markers or as linkage groups provide genetic information with high discrimination that is appropriate for forensic purposes with Paternity Informed Consent (PIC), Power of exclusion (PE), and Paternity index (PI) varied from 0.61211 to 0.917979, 0.38722 to 0.842949, and 0.038416 to 0.16367, respectively. A significant Linkage disequilibrium (LD) with p-value after Bonferroni correction p ≤ 0.05/66= 0.0008 was observed for 17 pairs of loci in male samples and 4 pairs of loci in female. In the male group, LG3 showed relatively high values of Haplotype diversity (HD). This indicated that these LGs were quite informative in the studied Saudi group and would have high application value in forensic sciences. The pairwise genetic distance fixation index (Fst) results showed that the Saudi population is genetically close to the Egyptian and Emirati populations and distant to the Turkish population. Genetic distances were represented in a non-metric MDS plot representing that Saudis cluster with Middle East populations and are clearly separated from European and East Asian populations. Conclusion:The current study revealed that Investigator® Argus 12 X-STR kit would support forensic application, kinship testing involving female offspring, and human identification in Saudi populations.

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Haplotype data and forensic evaluation of 23 Y-STR and 12 X-STR loci in eight ethnic groups from Eritrea

et al. 2020

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Eritrea is a multi-ethnic country of over 3 million of people consisting of different ethnic groups, having each its own language and cultural tradition. Due to the lack of population genetic data for markers of forensic interest, in this study, we analyzed the genetic polymorphisms of 23 Y-chromosome STR loci and of 12 X-chromosome STR loci in a sample of 255 unrelated individuals from 8 Eritrean ethnic groups, with the aim to generate a reference haplotype database for anthropological and forensic applications. X- and Y-chromosomes markers may indeed offer information especially in personal identification and kinship testing, when relying on the availability of large local population data to derive sufficiently accurate frequency estimates. The population genetic analyses in the Eritrean sample for both the two set of Y- and X-STR markers showed high power of discrimination both at country-based and population levels. Comparison population results highlight the importance of considering the ethnic composition within the analyzed country and the necessity of increasing available data especially when referring to heterogeneous populations such as the African ones.

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Genetic polymorphisms of twelve X-STRs of the investigator Argus X-12 kit and additional six X-STR centromere region loci in an Egyptian population sample

Cited by 38 publications

References 26 publications

Expanding X-chromosomal forensic haplotype frequencies database: Italian population data of four linkage groups

Expanding X-chromosomal forensic haplotype frequencies database: Italian population data of four linkage groups

Genetic diversity and haplotypic structure of a Saudi population sample using Investigator Argus X-12 amplification kit

Haplotype data and forensic evaluation of 23 Y-STR and 12 X-STR loci in eight ethnic groups from Eritrea

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