Genetic population structure of Peninsular bighorn sheep (Ovis canadensis nelsoni) indicates substantial gene flow across US–Mexico border

Buchalski, Michael R.; Navarro, Asako Y.; Boyce, Walter M.; Vickers, T. Winston; Tobler, Mathias W.; Nordstrom, Lisa A.; Garcia, J.; Gille, Daphne A.; Penedo, M. C. T.; Ryder, Oliver A.; Ernest, Holly B.

doi:10.1016/j.biocon.2015.01.006

Cited by 19 publications

(17 citation statements)

References 76 publications

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“…We observed statistically significant deviations from HWE in all clusters except for the Sierra Nevada subspecies, suggesting the presence of substructure in the remaining 4. This finding is not surprising given the spatial scale of our sampling, existing evidence of regional genetic structure among desert bighorn herds ( Epps et al 2010 ; Buchalski et al 2015 ), and our results for IBD tests.…”

Section: R Esultssupporting

confidence: 63%

“…Using the numerically largest and geographically broadest set of desert bighorn sheep samples analyzed to date, we found substantial genetic diversity throughout the native range. Observed heterozygosity and allelic richness were comparable or higher than other studies ( Gutierrez-Espeleta et al 2001 ; Epps et al 2005 , 2006 ; Buchalski et al 2015 ) and suggest desert bighorn retained substantial range-wide genetic diversity despite demographic declines and loss of population connectivity. The federally endangered Sierra Nevada population had low genetic diversity, consistent with recent bottlenecks and small size.…”

Section: Discussionsupporting

confidence: 50%

“…Total genomic DNA was extracted from blood, muscle, or skin tissue using Qiagen DNeasy Blood and Tissue kits (Qiagen Inc., Valencia, California) following the manufacturer’s protocol. Each sample was genotyped at 39 microsatellite loci described in Buchalski et al (2015) . Sex was confirmed via amplification of the Amelogenin marker described in Weikard et al (2006) .…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

“…We used a basic local alignment search tool ( BLAST — Altschul et al 1997 ) to search the nucleotide database in GenBank for all unique haplotypes present in our data, finding 35 homologous sequences for desert bighorn sheep, including accession nos. AF076911–AF076917 ( Boyce et al 1999 ), AY903993–AY904017 ( Epps et al 2005 ), KP688366–KP688368 ( Buchalski et al 2015 ), and AY116621–AY116623 (unpublished sequences for 2 Mexican and 1 Weems bighorn). We downloaded the archived sequences, preserving the original haplotype names, for inclusion in our phylogenetic analyses.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

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Phylogeographic and population genetic structure of bighorn sheep (Ovis canadensis) in North American deserts

Buchalski¹,

Sacks²,

Gille³

et al. 2016

JMAMMAL

Self Cite

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Fossil data are ambiguous regarding the evolutionary origin of contemporary desert bighorn sheep ( Ovis canadensis subspecies). To address this uncertainty, we conducted phylogeographic and population genetic analyses on bighorn sheep subspecies found in southwestern North America. We analyzed 515 base pairs of mtDNA control region sequence and 39 microsatellites in 804 individuals from 58 locations. Phylogenetic analyses revealed 2 highly divergent clades concordant with Sierra Nevada ( O. c. sierrae ) and Rocky Mountain ( O. c. canadensis ) bighorn and showed that these 2 subspecies both diverged from desert bighorn prior to or during the Illinoian glaciation (~315–94 thousand years ago [kya]). Desert bighorn comprised several more recently diverged haplogroups concordant with the putative Nelson ( O. c. nelsoni ), Mexican ( O. c. mexicana ), and Peninsular ( O. c. cremnobates ) subspecies. Corresponding estimates of effective splitting times (~17–3 kya), and haplogroup ages (~85–72 kya) placed the most likely timeframe for divergence among desert bighorn subspecies somewhere within the last glacial maximum. Median-joining haplotype network and Bayesian skyline analyses both indicated that desert bighorn collectively comprised a historically large and haplotype-diverse population, which subsequently lost much of its diversity through demographic decline. Using microsatellite data, discriminant analysis of principle components (DAPC) and Bayesian clustering analyses both indicated genetic structure concordant with the geographic distribution of 3 desert subspecies. Likewise, microsatellite and mitochondrial-based FST comparisons revealed significant fixation indices among the desert bighorn genetic clusters. We conclude these desert subspecies represent ancient lineages likely descended from separate Pleistocene refugial populations and should therefore be managed as distinct taxa to preserve maximal biodiversity. Los datos de fósiles sobre el origen evolutivo de las ovejas del desierto ( Ovis canadensis subespecies) contemporáneas son ambiguos. Para dilucidar esta incertidumbre, llevamos a cabo análisis filogeográficos y de genética de poblaciones entre cinco subespecies de ovejas del suroccidente de Norteamérica. Analizamos 515 pb de secuencia de la región control del ADN mitocondrial y 39 microsatélites en 804 ovejas de 58 localidades. Los análisis filogenéticos revelaron 2 clados altamente divergentes concordantes con ovejas de la Sierra Nevada ( O. c. sierrae ) y de las Montañas Rocosas ( O. c. canadensis ), y demostraron que estas dos subespecies divergieron antes o durante la glaciación de Illinois (315,000–94,000 años). Las ovejas del desierto formaron varios haplogrupos recientemente derivados concordantes con las subespecies de Nelson ( O. c. nelsoni ), México ( O. c. mexicana ) y peninsular ( O. c. cremnobates ). Las estimaciones correspondientes al tiempo de separación efectiva (17,000–3,000 años) y edades de haplogrupos (85,000–72,000 años) son los plazos más probables para las divergencia...

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Section: R Esultssupporting

confidence: 63%

Section: Discussionsupporting

confidence: 50%

Section: Aterials and M Ethodsmentioning

confidence: 99%

Section: Aterials and M Ethodsmentioning

confidence: 99%

See 2 more Smart Citations

Phylogeographic and population genetic structure of bighorn sheep (Ovis canadensis) in North American deserts

Buchalski¹,

Sacks²,

Gille³

et al. 2016

JMAMMAL

Self Cite

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show abstract

“…Even though we did not assess aquatic species, we note similar concerns about asymmetric management of fishes across international borders (Shackell, Frank, Nye, & den Heyer, ). Although peripheral populations may be most at risk from asymmetric protection, the reverse situation also is possible, where a more threatened core population benefits from potential rescue from a less protected but currently healthier peripheral population (Buschalski et al., ).…”

Section: Discussionmentioning

confidence: 99%

Asymmetric cross‐border protection of peripheral transboundary species

Thornton

Wirsing

López‐González

et al. 2017

CONSERVATION LETTERS

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International political boundaries challenge species conservation because they can hinder coordinated management. Peripheral transboundary species, those with a large portion of their range in one country and a small, peripheral portion in an adjacent country, may be particularly vulnerable to mismatches in management because peripheral populations are likely in greater conservation need than core populations. However, no systematic assessment of peripheral transboundary species or their status across borders has been attempted. We show that numerous species in three vertebrate taxa qualify as peripheral transboundary species in North America, and that these species are often protected differently across US-Canadian and US-Mexican borders. Asymmetries in cross-border protection may threaten populations through disruption of connectivity between periphery and core regions and are especially relevant given expected impacts of climate change and the US-Mexico border wall. Our results highlight the need for greater international collaboration in management and planning decisions for transboundary species. K E Y W O R D Sclimate change, conservation planning, international border, protection status, range, transboundary, vertebrates This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Large‐scale assessment of genetic structure to assess risk of populations of a large herbivore to disease

Walter,

Fameli,

Russo‐Petrick

et al. 2024

Ecology and Evolution

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Chronic wasting disease (CWD) can spread among cervids by direct and indirect transmission, the former being more likely in emerging areas. Identifying subpopulations allows the delineation of focal areas to target for intervention. We aimed to assess the population structure of white‐tailed deer (Odocoileus virginianus) in the northeastern United States at a regional scale to inform managers regarding gene flow throughout the region. We genotyped 10 microsatellites in 5701 wild deer samples from Maryland, New York, Ohio, Pennsylvania, and Virginia. We evaluated the distribution of genetic variability through spatial principal component analysis and inferred genetic structure using non‐spatial and spatial Bayesian clustering algorithms (BCAs). We simulated populations representing each inferred wild cluster, wild deer in each state and each physiographic province, total wild population, and a captive population. We conducted genetic assignment tests using these potential sources, calculating the probability of samples being correctly assigned to their origin. Non‐spatial BCA identified two clusters across the region, while spatial BCA suggested a maximum of nine clusters. Assignment tests correctly placed deer into captive or wild origin in most cases (94%), as previously reported, but performance varied when assigning wild deer to more specific origins. Assignments to clusters inferred via non‐spatial BCA performed well, but efficiency was greatly reduced when assigning samples to clusters inferred via spatial BCA. Differences between spatial BCA clusters are not strong enough to make assignment tests a reliable method for inferring the geographic origin of deer using 10 microsatellites. However, the genetic distinction between clusters may indicate natural and anthropogenic barriers of interest for management.

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Genetic population structure of Peninsular bighorn sheep (Ovis canadensis nelsoni) indicates substantial gene flow across US–Mexico border

Cited by 19 publications

References 76 publications

Phylogeographic and population genetic structure of bighorn sheep (Ovis canadensis) in North American deserts

Phylogeographic and population genetic structure of bighorn sheep (Ovis canadensis) in North American deserts

Asymmetric cross‐border protection of peripheral transboundary species

Large‐scale assessment of genetic structure to assess risk of populations of a large herbivore to disease

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