Genetic Profile and Serosurvey for Virus Infections of Japanese Wild Boars in Shikoku Island

Ishiguro, Naotaka; Nishimura, Masakazu

doi:10.1292/jvms.67.563

Cited by 14 publications

(33 citation statements)

References 11 publications

(23 reference statements)

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“…When the 77 mtDNA haplotypes were compared with previously reported mtDNA sequences, the haplotype of sample No. 2007E45, isolated from Lombok Island, was identical to the haplotype M33 from domestic pig (Ishiguro and Nishimura 2005).…”

Section: Resultsmentioning

confidence: 87%

“…Neighbor-joining (NJ) phylogenetic tree constructed using the 572-bp to 574-bp mtDNA control region. This tree includes 76 novel mtDNA haplotypes from Indonesia identified in this study and 81 previously reported haplotypes (Hongo et al 2002;Ishiguro and Nishimura 2005;Ishiguro et al 2008a). Indonesian mtDNA haplotypes are the same as listed in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…The mtDNA D-loop region (572 to 574 bp) was amplified using two primers, mit112 and mit106 (Watanobe et al 2003). PCR products were purified using a QIAquick PCR Purification kit (Qiagen), as described by Ishiguro and Nishimura (2005). Sequence reaction of the purified PCR products was then performed using two primers (mit11 and mit12) and a BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems), followed by automated sequencing on an ABI 310 Genetic Analyzer.…”

Section: Dna Extraction and Mtdna Analysismentioning

confidence: 99%

“…Alignment of DNA sequences and phylogenetic analysis mtDNA sequences (572 to 574 bp) were aligned with haplotypes published previously (Hongo et al 2002;Watanobe et al 2003;Ishiguro and Nishimura 2005;Ishiguro et al 2008a) using Clustal W (gap opening penalty 15, gap extension penalty 6.66) (Thompson et al 1994). Genetic distance was estimated by the proportion (P)-distance method in the MEGA4 (Tamura et al 2007).…”

Section: Dna Extraction and Mtdna Analysismentioning

confidence: 99%

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mtDNA Variation and Human-Mediated Introgression of IndigenousSusPopulations on Several Indonesian Islands

Ishiguro¹,

Inoshima²,

Sasaki

et al. 2012

Mammal Study

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Abstract. To examine the genetic origin of the domestic pig, the distribution of wild boar, and human-mediated translocation of the domestic pig, we collected 223 samples from domestic pigs and wild boars from eight Indonesian islands, sequenced the control region of mitochondrial DNA (mtDNA) from each sample, and compared these sequences with previously determined sequences from East and Southeast Asian domestic pigs and wild boars. Three Sus species (S. scrofa, S. verrucosus, and S. celebensis) were identified on the Indonesian islands. The mtDNA sequences of three Indonesian Sus species were diverse, and they clustered into three lineages with low bootstrap values (an S. scrofa group including East and Southeast Asian domestic pigs and wild boars, a group including indigenous S. scrofa together with S. verrucosus from Sumatra and Java Islands, and an S. celebensis group from Sulawesi Island). The mtDNA haplotypes of S. scrofa wild boars from three (Sumbawa, Flores and New Guinea) islands and domestic pigs from two (Lombok and Timor) islands east of the Wallace Line, and some S. scrofa wild boars from Sumatra and Java Islands were related to Vietnamese pig mtDNA sequences in the East and Southeast Asian domestic pig and wild boar clade, supporting that ancient immigrants likely introduced domestic pigs from the Asian continent to east Indonesian islands. The mtDNA haplotypes of S. celebensis were broadly divided into three groups, which were distributed in the north and southwest areas, central area and southeast area of Sulawesi Island.

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Dna Extraction and Mtdna Analysismentioning

confidence: 99%

Section: Dna Extraction and Mtdna Analysismentioning

confidence: 99%

See 2 more Smart Citations

mtDNA Variation and Human-Mediated Introgression of IndigenousSusPopulations on Several Indonesian Islands

Ishiguro¹,

Inoshima²,

Sasaki

et al. 2012

Mammal Study

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“…However, the reported seroprevalences of PRV in wild boars in western (10%) and eastern (9%) Germany [10,12], France (6%) [1], south-central Spain (56%) [5], Croatia (55%) [16] and the southern U.S.A. (34-61%) [2,6,7,14] seem to be higher than that in Japan (3%). In addition, only one previous report indicated that wild boars in Shikoku, in southern Japan, were free from PRV [8]. These results suggest that the low seroprevalence of PRV in wild boars in Japan might be indicative of the success of the PRV eradication program in domestic pigs.…”

mentioning

confidence: 80%

Pseudorabies Virus Infection in Wild Boars in Japan

et al. 2011

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ABSTRACT. In Japan, most pig populations are now free from pseudorabies virus (PRV) due to the recent success of an extensive eradication program. However, PRV infection persists in Japanese wild boars (Sus scrofa leucomystax), representing another potential reservoir for the virus in Japan. In this study, the seroprevalence of PRV in wild boars captured in three different prefectures was ascertained. A virus neutralization (VN) test showed that 6 of 173 serum samples (3%) were positive for VN antibody; glycoprotein E-ELISA revealed infection with the wild-type, but not the available vaccine strain, PRV. These results indicate that PRV has continued to spread among wild boars in Japan.KEY WORDS: Aujeszky's disease, pseudorabies virus, wild boar.J. Vet. Med. Sci. 73(11): 1535-1537, 2011 Pseudorabies virus (PRV), a member of the genus Varicellovirus in the subfamily Alphaherpesvirinae of the family Herpesviridae [9], is the causative agent of a contagious and epidemic disease, Aujeszky's disease, which affects swine at various production phases, causing high mortality in naive and newborn piglets and abortion in pregnant sows. This disease results in significant economic losses for the swine industry [3].In Japan, a PRV eradication program in domestic pigs has been successfully implemented; consequently, most domestic pig populations are now free from wild-type PRV and therefore are not vaccinated with a live attenuated vaccine. As this vaccine virus has a deletion in the glycoprotein E (gE) gene, vaccinated pigs can be serologically differentiated from those infected with wild-type PRV by the gE-ELISA. In 1997, a PRV epidemic in Nara Prefecture, Japan, resulted in the deaths of 24 hunting dogs due to PRV infection acquired after eating raw wild boar (Sus scrofa leucomystax) meat (Kouda, T. et al., In the 129th Annual Meeting of the Japanese Society of Veterinary Science, Tsukuba, 2000). This event suggested that wild boars may represent a potential source of PRV infection for other animals. However, there is currently little information about the present situation of PRV prevalence among wild boars in Japan. Here, we present the results of a seroepidemiological study of PRV in wild boars in Japan in order to clarify the seroprevalence of PRV in this population.A total of 173 serum samples were collected from wild boars in three different prefectures A, B and C, of Japan (Table 1). These three prefectures were located in the western part of Japan, were not next to each other and were free from PRV in their pig populations. Most animals were hunted with government permission during the winter season. Sera were inactivated by incubation at 56C for 30 min and then kept at -20C until use.Cloned porcine kidney (CPK) cells were kindly provided by the National Institute of Animal Health in Japan; these were cultured in Dulbecco's Modified Eagle's Medium (DMEM; GIBCO, NY, U.S.A.) supplemented with 10% fetal calf serum (FCS), 100 U/ml penicillin and 100 μg/ml streptomycin. Subsequently, the PRV Indiana strain was propag...

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Pseudorabies virus in wild swine: a global perspective

et al. 2011

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Suid herpesvirus 1 (SuHV1, syn. Aujeszky's disease virus [ADV] or pseudorabies virus [PrV]), which belongs to the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus is the causative agent of Aujeszky's disease (AD, pseudorabies), a notifiable disease, that causes substantial economic losses to the swine industry in countries, where AD is present. Members of the family Suidae (true pigs) are the only natural hosts for PrV, although the virus can infect numerous other mammals including ruminants, carnivores and rodents. Despite the tremendous progress that has been made in controlling and eliminating PrV in domestic pigs, there is mounting evidence that PrV infections are more widespread in wild swine across the world than originally thought. Unfortunately, our understanding of the extent of PrV infections in these wild populations and of the threat to domestic swine is still fragmentary. This review aims at giving a global perspective on PrV infections in wild swine by scrutinizing the current state of knowledge concerning (i) the global occurrence of PrV infections in free-living populations of wild swine, e.g., wild boar and feral swine, (ii) the molecular characterization of wild swine PrV, (iii) infection characteristics of PrV in populations of wild swine, (iv) the risk of spillover infections to domestic pigs, (v) potential risk-mitigating measures, focusing on further research needs.

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Genetic Profile and Serosurvey for Virus Infections of Japanese Wild Boars in Shikoku Island

Cited by 14 publications

References 11 publications

mtDNA Variation and Human-Mediated Introgression of IndigenousSusPopulations on Several Indonesian Islands

mtDNA Variation and Human-Mediated Introgression of IndigenousSusPopulations on Several Indonesian Islands

Pseudorabies Virus Infection in Wild Boars in Japan

Pseudorabies virus in wild swine: a global perspective

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