Genetic profile characterization of 10 X-STRs in four populations of the southeastern region of Brazil

Abstract

Ten X-chromosomal short tandem repeats (DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08 and DXS7423) were analyzed in four populations of the southeastern region of Brazil (São Paulo, Rio de Janeiro, Vitória and Belo Horizonte). No deviations from the Hardy-Weinberg equilibrium were observed for any of the analyzed loci in the four populations. The average diversity per locus varied between 68% for DXS8378, DXS7133, and DXS7423 and 83%, for DXS6809, with Rio de Janeiro being the most diverse population. Overall power of discrimination values in females varied between 0.99999999990 and 0.99999999997 and between 0.9999991 and 0.9999995 in males. These high values show the potential of this system for forensic application and relationships' testing in the studied groups. Genetic comparisons (exact tests of population differentiation and pairwise genetic distances) revealed significant differences between Brazilian and other populations from Europe, Latin America and Africa, as well as among different Brazilian populations.

Extensive survey of 12 X-STRs reveals genetic heterogeneity among Brazilian populations

“…In recent years, many studies built and validated panels of STRs markers on the X chromosome (X-STR markers) to be used in forensic genetics, usually employing polymerase chain reaction (PCR) multiplexes ranging from six to 12 STR per reaction [3][4][5][6][7][8]. Such panels have been useful in complex relatedness cases when [9][10][11] (1) the putative father was not available, (2) the relatives were consanguineous, (3) the relative of the putative father is either his mother or daughter, and (4) in maternity investigation of a son [12].…”

DNA human identification for the family rights: One year of agreement between UNESP/Laboratory of Paternity and Public Defender Service in São Paulo State

“…When necessary, 12 Y-STR (PowerPlex 1 Y System, Promega) and 10 X-STR [1] markers were analyzed. The latter were amplified as proposed by Martins et al [2]. The PCR products were separated on an Applied Biosystems 3500 Genetic Analyzer and genotyping was performed using GeneMapper 1 ID-X Software (Applied Biosystems).…”

Aplicabilidade do cromossomo X no DNA forense

“…O polimorfismo presente nesse cromossomo vem atraindo cada dia mais a atenção de pesquisadores. Inúmeros estudos na população brasileira demonstraram que vários marcadores moleculares situados no cromossomo X já são validados para uso forense, sendo que estes apresentam resposta no que tange sobre a variabilidade desses polimorfismos na população (CAINÉ et al, 2007;CAINÉ et al, 2010;GUSMÃO et al, 2009;LEITE et al, 2009;MARTINS et al, 2008a;MARTINS et al, 2009;MARTINS et al, 2010a;MARTINS et al, 2010b;PENNA et al, 2011;RODRIGUES et al, 2010;RODRIGUES et al, 2011;SILVA et al, 2010;TAVARES et al, 2008). Na rotina forense, os marcadores do tipo STR mais utilizados são os localizados nos cromossomos autossômicos e no Y (TOMAS et al, 2015).…”