Genetic Profiles of 12 Inbred Rat Strains for 46 Microsatellite Loci Selected as Genetic Monitoring Markers

Hirayama, Nobuyuki; Kuramoto, Takashi; Kondo, Yasushi; Yamada, Junzo; Serikawa, Tadao

doi:10.1538/expanim1978.43.1_129

Cited by 6 publications

(2 citation statements)

References 1 publication

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“…The DNA samples were prepared from the tails of the mice using GENEXTRACTOR TA-100 (Takara Shuzo Co., Ltd., Shiga, Japan). The PCR method was performed as described previously (Hirayama et al, 1994). Briefly, 25 ml of reaction mixture containing DNA, 20 mM Tris-HCl, 50 mM KCl, 1-4 mM MgCl 2 , 200 mM dNTP mixture, 0.5 mM PCR primers, and 0.5 U Taq DNA polymerase (GibcoBRL, Grand Island, NY) in each tube was covered with a drop of mineral oil.…”

Section: Preparation Of Samples For Genetic Analysesmentioning

confidence: 99%

Effects of cryopreservation of mouse embryos and in vitro fertilization on genotypic frequencies in colonies

Goto

Muguruma

Kuramochi

et al. 2002

Molecular Reproduction Devel

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To evaluate the effects of cryopreservation and in vitro fertilization (IVF) on genotypic frequencies in mouse colonies, genotypic frequencies at 15 biochemical, 4 immunological and 20 microsatellite loci were examined in three colonies of MCH (ICR) mice derived from noncryopreserved embryos obtained by natural mating without the induction of superovulation, cryopreserved embryos obtained by natural mating with the induction of superovulation, and cryopreserved embryos obtained by the induction of superovulation and IVF. Three (Pgm-1, Ldr-1 and Hbb) out of the 15 biochemical loci, two (Thy-1 and H2K) out of four immunological loci and five (D5Mit18, D6Mit15, D12Mit5, D13Mit26, and D14Mit7) out of 20 microsatellite loci that showed polymorphisms in every colony were used for detection of genotypic frequencies. The genotypic frequencies of the loci in the three colonies did not differ from the predicted genotypic frequencies (P > 0.05). The results suggested that genetic drift does not occur among colonies established from treated and untreated embryos, and it was clear that the embryo banking by cryopreservation is suitable for preservation of outbred stock without genetic drift.

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Section: Preparation Of Samples For Genetic Analysesmentioning

confidence: 99%

Effects of cryopreservation of mouse embryos and in vitro fertilization on genotypic frequencies in colonies

Goto

Muguruma

Kuramochi

et al. 2002

Molecular Reproduction Devel

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show abstract

“…A titer is determined by testing constant numbers of cells against serial dilutions of antisera and reporting the lowest dilution at which agglutination can be recognized. Microsatellite panels were developed for mice and rats, and the use of SSLPs to analyze murine DNA for genetic contamination was widespread until recently and is still in use today (Montagutelli et al, 1991;Hirayama et al, 1994;Kloting et al, 1995). If they are lysed or otherwise damaged by the presence of alloantibodies, they lose their ability to exclude a dye (Gorer and O'Gorman, 1956) or they refract glycerine differently (Shiroishi et al, 1981).…”

Section: And 312)mentioning

confidence: 99%

Genetic Monitoring of Laboratory Mice and Rats

Strobel

Reinholdt

Malcolm

et al. 2015

Laboratory Animal Medicine

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Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains

et al. 1995

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Genetic monitoring is an essential component of colony management and for the rat has been accomplished primarily by using immunological and biochemical markers. Here, we report that simple sequence length polymorphisms (SSLPs) are a faster and more economical way of monitoring inbred strains of rats. We characterized 61 inbred strains of rats, using primer pairs for 37 SSLPs. Each of these loci appeared to be highly polymorphic, with the number of alleles per locus ranging between 3 and 14 and, as a result, all the 61 inbred strains tested in this study could be provided with a unique strain profile. These strain profiles are also used for estimating the degree of similarity between strains. This information may provide the rationale in selecting strains for genetic crosses or for other specific purposes.

show abstract

Genetic Profiles of 12 Inbred Rat Strains for 46 Microsatellite Loci Selected as Genetic Monitoring Markers

Cited by 6 publications

References 1 publication

Effects of cryopreservation of mouse embryos and in vitro fertilization on genotypic frequencies in colonies

Effects of cryopreservation of mouse embryos and in vitro fertilization on genotypic frequencies in colonies

Genetic Monitoring of Laboratory Mice and Rats

Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains

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