Genetic Profiling of<i>Hypericum</i>(St. John’s Wort) Species by Nuclear Ribosomal<i>ITS</i>Sequence Analysis

Crockett, Sara L.; Douglas, Andrew W.; Scheffler, Brian E.; Khan, Ikhlas A.

doi:10.1055/s-2004-832619

Cited by 35 publications

(48 citation statements)

References 9 publications

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“…Ascyrum. On the contrary, ITS sequence data proved the monophyly of Myriandra (Crockett et al 2004). The inconsistencies between gene trees based on nuclear and plastid markers are not uncommon and could be attributed to lineage sorting, hybridization / reticulation, nonhomologous sampling of duplicated gene, or chloroplast capture (Hendy & Penny 1989;Doyle 1997;Maddison 1997;Sang & Zhong 2000;Tsitrone et al 2003; …”

Section: Pcr Amplifications and Restriction Of Pcr Productsmentioning

confidence: 99%

“…Despite the economical potential of the Hypericum species, there are only two references on the application of molecular markers in Hypericum: phylogenetic studies both employing nuclear rDNA ITS sequence data (Crockett et al 2004;Park & Kim 2004). Preliminary PCR-RFLP analysis of cpDNA showed a considerable degree of interspecific polymorphism and confirmed the applicability of this method in phylogenetic investigations in the genus Hypericum (Hazler Pilepić 2002).…”

Section: Introductionmentioning

confidence: 98%

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RFLP analysis of cpDNA in the genus Hypericum

et al. 2010

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The chloroplast DNA of 43 species including 16 sections from the genus Hypericum was studied by PCR-RFLP analysis. The PCR-amplified products of four cpDNA regions, trnC-trnD, psbC-trnS, trnL-trnF and rbcL were digested with four restriction endonucleases. A high level of interspecific variation was detected while intraspecific diversity was not observed. The resulting parsimony analysis indicated the monophyletic assemblage of the sections Androsaemum, Olympia, Drosocarpium and Trigynobrathys. Monophyly of Hypericum is weakly supported, but close relationships of H. perforatum and H. maculatum are indicated. The members of Ascyreia are weakly resolved, but clustering of H. kouytchense and H. oblongifolium is well supported, however, H. reptans is nested with Olympia. CpDNA profiles and the positions on the parsimony tree indicate that the chloroplast donor among the putative parents of the hybrid species H. ×inodorum is H. androsaemum.

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Section: Pcr Amplifications and Restriction Of Pcr Productsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

RFLP analysis of cpDNA in the genus Hypericum

et al. 2010

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“…According to Howard et al ., the nuclear ribosomal Internal transcribed spacer (ITS1 and ITS2) DNA region is the most suitable ‘‘barcode’’ for primer creation (through PCR), plant species distinguishing and detection of adulterants. Many DNA barcoding studies on Hypericum species identification have been conducted including those of Crockett et al ., Park and Kim, Howard et al ., Pilepic et al . and Costa et al …”

Section: Dna Barcodingmentioning

confidence: 99%

“…The authors proposed this technique for the authentication of SJW in commercial products (species level), but they concluded that the technique was not sufficient on subspecies level. The technique was also useful for evaluation of phylogenetic affinities within the genus …”

Section: Dna Barcodingmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives The most widely applied qualitative and quantitative analytical methods in the quality control of Hypericum perforatum extracts will be reviewed, including routine analytical tools and most modern approaches. Key findings Biologically active components of H. perforatum are chemically diverse; therefore, different chromatographic and detection methods are required for the comprehensive analysis of St. John's wort extracts. Naphthodianthrones, phloroglucinols and flavonoids are the most widely analysed metabolites of this plant. For routine quality control, detection of major compounds belonging to these groups seems to be sufficient; however, closer characterization requires the detection of minor compounds as well. Conclusions TLC and HPTLC are basic methods in the routine analysis, whereas HPLC‐DAD is the most widely applied method for quantitative analysis due to its versatility. LC‐MS is gaining importance in pharmacokinetic studies due to its sensitivity. Modern approaches, such as DNA barcoding, NIRS and NMR metabolomics, may offer new possibilities for the more detailed characterization of secondary metabolite profile of H. perforatum extracts.

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“…This could be attributed to the fact that the cultivated varieties used in this study were developed in Germany and Denmark. Crockett et al (2004) study provides a genetic method for authentication of commercial H. perforatum preparations and allows a preliminary assessment of phylogenetic relationships within the genus, revealing three strongly supported monophyletic clades, plus several secondary monophyletic groupings. Using internal transcribed spacer (ITS) gene sequences, they were able to distinguish H. perforatum from all other species of Hypericum included in this study.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis in the Hypericum perforatum populations in the Kashmir valley by using inter-simple sequence repeats (ISSR) markers

Shazia¹,

M.²,

P.³

et al. 2014

Afr. J. Biotechnol.

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Assessment of genetic variability among the Hypericum perforatum populations is critical to the development of effective conservation strategies in the Kashmir valley. To obtain accurate estimates of genetic diversity among and within populations of H. perforatum, inter-simple sequence repeats (ISSR) markers were used. The study was aimed to check, whether ISSR fingerprinting may be a useful tool for studying genetic variations among H. perforatum populations in the Kashmir valley (India). A total of 15 ISSR primers were tested with the 20 genotypes of H. perforatum. The ten informative primers were selected and used to evaluate the degree of polymorphism and genetic relationships within and among all the H. perforatum populations. ISSR of 20 genotypes analysis yielded 98 fragments that could be scored, of which 71 were polymorphic, with an average of 7.1 polymorphic fragments per primer. Number of amplified fragments varied in size from 150 to 1650 bp. Percentage of polymorphism ranged from 60% to a maximum of 100%. Resolving power ranged from a minimum of 7.7 to a maximum of 14.3. Shannon indexes ranges from 0.166 to 0.389 with an average of 0.198 and Nei's genetic diversity (h) ranges from 6.98 to 9.8. Estimated value of gene flow (Nm = 0.579) indicated that there was limited gene flow among the populations. The genetic diversity (Ht) within the population of 0.245 was clearly higher than that of among population genetic diversity (Hs= 0.115), indicating an out-crossing predominance in the studied populations. Analysis of molecular variance by ISSR markers indicated that over half of the total variation in the studied populations (58%) could be accounted for by differences among the 8 divisions, with a further 42% being accounted for by the variation among populations within a division.The dendrogram grouping the populations by unweighted pair-group method with arithmetic averages (UPGMA) method revealed eight main clusters. In conclusion, combined analysis of ISSR markers and hypericin content is an optimal approach for further progress and breeding programs.

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Genetic Profiling ofHypericum(St. John’s Wort) Species by Nuclear RibosomalITSSequence Analysis

Cited by 35 publications

References 9 publications

RFLP analysis of cpDNA in the genus Hypericum

RFLP analysis of cpDNA in the genus Hypericum

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Genetic diversity analysis in the Hypericum perforatum populations in the Kashmir valley by using inter-simple sequence repeats (ISSR) markers

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