Genetic purity of common bean seed generations (Phaseolus vulgaris cv. 'INTA ROJO') as tested with microsatellite markers

Jiménez, Oswalt R.; Korpelainen, Helena; Rojas, Antonio; Elomaa, Paula; Valkonen, Jari P. T.

doi:10.15258/sst.2012.40.1.08

Cited by 5 publications

(4 citation statements)

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“…‘DICTA 105’. It is one of the most important bean cultivars in Nicaragua owing to its high yield, drought tolerance, adaptability to different environmental conditions, red skin (preferred by local consumers), good flavor, and short cooking time [5,6]. …”

Section: Introductionmentioning

confidence: 99%

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Marcenaro

Valkonen

2016

PLoS ONE

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Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, ‘INTA Rojo’. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions. Eighty-seven isolates caused symptoms of varying severity in the seedlings, including discoloration, necrotic lesions, cankers, rot, and lethal necrosis. Pathogenic isolates were divided into eight phenotypically distinguishable groups based on morphology and growth characteristics on artificial growth medium, and further identified by analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of the ribosomal RNA genes. The pathogenic isolates belonged to eight genera. Fusarium spp. (F. chlamydosporum, F. equiseti, F. incarnatum), Lasiodiplodia theobromae, Macrophomina phaseolina, and Penicillium citrinum were the most damaging and common fungi found in the seed lots. Furthermore, Corynespora cassiicola, Colletotrichum capsisi, Colletotrichum gloeosporioides, Aspergillus flavus, and Diaporthe sp. (Phomopsis) were seedborne in cultivar ‘INTA Rojo’ and found to be pathogenic to bean seedlings. This study reveals, for the first time, many seedborne pathogenic fungi in beans in Nicaragua; furthermore, prior to this study, little information was available concerning F. equiseti, F. incarnatum, L. theobromae, C. cassiicola, and Diaporthe spp. as seedborne pathogens of common bean. Our results lay the basis for developing diagnostic tools for seed health inspection and for further study of the epidemiology, ecology, and control of the pathogenic fungi of common beans in the field.

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Section: Introductionmentioning

confidence: 99%

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Marcenaro

Valkonen

2016

PLoS ONE

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“…This cultivar has been one of the most yielding cultivar in Nicaragua in the last decade, and it has been proposed as a reference for yield comparisons (Jiménez and Korpelainen , Jiménez et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…The DNA extraction of 60 F 1 plants and ten individuals from each parent line was carried out following Jiménez et al. (). With the purpose of quantifying the level of heterozygosity, microsatellite genotyping was carried out in the laboratory of the Department of Agricultural Sciences, University of Helsinki, Finland.…”

Section: Methodsmentioning

confidence: 99%

Preliminary evaluation of F₁ generation derived from two common bean landraces (Phaseolus vulgaris) from Nicaragua

Jiménez

Korpelainen

2013

Plant Breeding

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Germplasm improvement is a conceivable solution for increasing food production in developing countries. The objectives of the present study were to incorporate the genetic diversity of two Nicaraguan common bean landraces into a segregating population and to evaluate its potential as breeding material. Adapted landraces PV0006 and PV0023 were selected as parents for single crosses based on molecular and phenotypic characteristics. The results indicate that we succeeded in reshuffling most of the genetic diversity of both parents into a segregating population. The level of allele recombinations suggests that theoretically between 128 and 1024 different pure lines could be obtained. We found putative associations between alleles and yield‐determinant components. Computer simulations demonstrated that the pedigree method and the marker selection of the most segregating individuals in the F1 generation could improve the genetic gains for yield. These findings expose the potential of this segregating population and the feasibility of local landraces for bean improvement.

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“…The main bean cultivars that are produced in Nicaragua nowadays were improved by regional bean breeding programmes using germplasm from different sources (Jiménez, 2009). These programmes produce new cultivars for the Central American region where the preferences and environmental conditions vary among different countries.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite markers reveal promising genetic diversity and seed trait associations in common bean landraces (Phaseolus vulgarisL.) from Nicaragua

Jiménez

Korpelainen

2012

Plant Genet. Res.

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Nicaragua is located in the Mesoamerican diversity centre for common beans (Phaseolus vulgarisL.). Yet, there is insufficient knowledge of the molecular characteristics of most common bean landraces in Nicaragua. The objectives of the present study were to investigate the genetic diversity of common bean landraces and to identify promising sources of genetic variation for breeding purposes. Two cultivars and 40 landraces never studied before were selected from a collection based on the geographic origin, seed coloration and information provided by farmers. Fourteen microsatellite markers distributed in different linkage groups were analysed. The study revealed that there is a high genetic diversity (mean 8.9 alleles per locus). The populations showed structuring into three groups where seed weight had a strong relationship with population clustering. At least 20% of the populations hold promising allelic variation and potential for good market acceptance that could be maximized in breeding programmes. Additionally, four markers revealed a high correlation with seed length, width and weight, suggesting that marker-assisted selection for these yield-determinant traits could be straightforward. Nonetheless, more marker–trait associations should be addressed in order to enforce this practice.

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Genetic purity of common bean seed generations (Phaseolus vulgaris cv. 'INTA ROJO') as tested with microsatellite markers

Cited by 5 publications

References 0 publications

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Preliminary evaluation of F₁ generation derived from two common bean landraces (Phaseolus vulgaris) from Nicaragua

Microsatellite markers reveal promising genetic diversity and seed trait associations in common bean landraces (Phaseolus vulgarisL.) from Nicaragua

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Genetic purity of common bean seed generations (Phaseolus vulgaris cv. 'INTA ROJO') as tested with microsatellite markers

Cited by 5 publications

References 0 publications

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Preliminary evaluation of F1 generation derived from two common bean landraces (Phaseolus vulgaris) from Nicaragua

Microsatellite markers reveal promising genetic diversity and seed trait associations in common bean landraces (Phaseolus vulgarisL.) from Nicaragua

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Preliminary evaluation of F₁ generation derived from two common bean landraces (Phaseolus vulgaris) from Nicaragua