Genetic reagents for making split-GAL4 lines in Drosophila

Abstract

The ability to reproducibly target expression of transgenes to small, defined subsets of cells is a key experimental tool for understanding many biological processes. The Drosophila nervous system contains thousands of distinct cell types and it has generally not been possible to limit expression to one or a few cell types when using a single segment of genomic DNA as an enhancer to drive expression. Intersectional methods, in which expression of the transgene only occurs where two different enhancers overlap in their expression patterns, can be used to achieve the desired specificity. This report describes a set of over 2,800 transgenic lines for use with the split-GAL4 intersectional method.

A genetic, genomic, and computational resource for exploring neural circuit function

“…Our approach requires genetic driver lines to obtain transcriptomes of specific cell populations. The recent availability of large collections of reagents for split-GAL4 intersections (Dionne et al, 2018;Tirian and Dickson, 2017) make it possible to obtain such lines for virtually any cell type of interest. This expanding genetic toolbox works well with our TAPIN-seq method to profile transcriptomes.…”

“…Cell type-specific drivers also facilitate follow-up experiments, for example evaluating the role of individual genes in individual cells. In Drosophila, large collections of GAL4 driver lines (Jenett et al, 2012;Tirian and Dickson, 2017) and the possibility to further refine these patterns with intersectional methods such as split-GAL4 (Luan et al, 2006;Dionne et al, 2018) enable genetic access to many neuronal populations (see, for example, Tuthill et al, 2013;Aso et al, 2014;Wu et al, 2016). We therefore chose the genetic, rather than single cell, approach to build a genomics resource to explore circuit function.…”

Neurogenetic dissection of the Drosophila innate olfactory processing center

“…Hemidriver lines were created using gateway cloning as previously described (Dionne et al 2018) . All transgenic fly lines were generated by either Bestgene Inc or Genetic Services, Inc.…”

The Neural Basis for a Persistent Internal State inDrosophilaFemales

“…from the Andolfatto group at Columbia University,Dsx-GAL4 (Rideout et al, 2010) andDsx-Gal4.DBD (Pavlou et al., 2016) from Stephen Goodwin, R71G01-p65.AD;MKRS/TM6B,tb (#70798;(Dionne et al, 2018)) and GMR57C10-LexA (#52817) from Gerry Rubin, 10xUAS-Syn21-Chrimson-tdTomato 3.1 [attP18], 13xLexAop2-IVS-Syn21-opGCaMP6s from Allan M. Wong(Hoopfer et al, 2015).w+,NorpA[36],20xUAS-csChrimson-mVenus[attp18];CyO/Sp;MKRS/TM6B,tb is from Vivek Jayaraman, VT25602.p65ADZp; VT2064.ZpGAL4DBD (Wu et al, 2019) and UAS>STOP>TNT (Stockinger et al, 2005) from Barry Dickson, R41A01-LexA (Zhou et al, 2014), Dsx-LexA::P65 (Zhou et al, 2015) from Bruce Baker, 8xLexAop-mCD8tdTomato from Yuh Nung Jan, and UAS(FRT.mCherry)ReachR [attp5] (now Bloomington #53743) from David Anderson (Inagaki et al, 2014). The following flies came from the Bloomington Drosophila stock center: UAS-2xEGFP;Dsx-Gal4 (#6874), R71G01-LexA::p65 [attp40] (Pan et al, 2012) (#54733), w[*]; P{UAS(FRT.w[+mW.hs])TeTxLC}10/CyO (#28842; Keller et al 2002), 8xLexAop-FLP [attp2] ( #55819) and 13xLexAop-GCaMP6s (#44590)…”